Enhanced anti-influenza agents conjugated with anti-inflammatory activity

ABSTRACT

Novel dual-targeted, bifunctional anti-influenza drugs formed by conjugation with anti-inflammatory agents are disclosed. Exemplary drugs according to the invention include caffeic acid (CA)-bearing zanamivir (ZA) conjugates ZA-7-CA (1), ZA-7-CA-amide (7) and ZA-7-Nap (43) for simultaneous inhibition of influenza virus neuraminidase and suppression of proinflammatory cytokines. Synthetic methods for preparation of these enhanced anti-influenza conjugate drugs are provided. The synthetic bifunctional ZA conjugates act synergistically towards protection of mice lethally infected by H1N1 or H5N1 influenza viruses. The efficacy of ZA-7-CA, ZA-7-CA-amide and ZA-7-Nap conjugates is much greater than the combination therapy of ZA with anti-inflammatory agents.

RELATED APPLICATION

This application claims priority to U.S. Provisional Application No. 61/624,250 under 35 U.S.C. 119(e), filed on Apr. 14, 2012, the content of which is hereby incorporated by reference in its entirety.

TECHNICAL FIELD OF THE INVENTION

The invention relates to the field of antiviral therapy. Specifically, the invention relates to bifunctional therapeutics directed to influenza virus infection wherein the therapeutic drug comprises an anti-influenza moiety conjugated to an anti-inflammatory agent.

BACKGROUND OF THE INVENTION

Influenza is a respiratory infection that causes severe health problems. The worldwide spread of the A/H5N1 avian flu and the outbreak of the new type A/H1N1 human influenza in 2009 have increased public awareness of the potential for global influenza pandemics. H5N1 influenza virus appears to replicate more rapidly than seasonal influenza and the pandemic H1N1/2009 strain. The H5N1 virus infection of macrophages also causes strong expression of proinflammatory cytokines and chemokines, namely cytokine storm. (Lee, S. M. Y. et al. J. Infect. Dis. 2008, 198, 525; Woo, P. C. Y. et al. J. Infec. Dis. 2010, 201, 346)

In the patients of H5N1 virus infection, induction of tumor necrosis factor (TNF)-α and interferons (IFN)-β/γ have been observed in alveolar and bronchial epithelial cells. (Yuen, K. Y. et al. Hong Kong Med. J. 11, 189 (2005)) Other cytokines, such as interleukins (e.g. IL-6 and IL-10), IFN-induced chemokines (e.g. IP-10, MIG and MIG-1) are also found at high levels in the H5N1 virus infected patients. (de Jong, M. D. et al. Nat Med 2005, 11, 189) The high mortality of human infected by influenza H5N1 has been attributed to poor response of virus to neuraminidase (NA) inhibitor, e.g. oseltamivir, and the excessive induction of a severe cytokine storm. (Peiris, J. S. M. et al. Lancet 2004, 363, 617; Geiler, J., Med. Microbiol. Immunol. 2011, 200, 53) Though treatment of the H5N1 virus infected mice with anti-inflammatory agent alone can inhibit proinflammatory cytokines, (Fedson, D. S. Influenza Other Resp. 2009, 3, 129) the mortality of infected mice is not reduced. (Carter, M. J. A J. Med. Microbiol. 2007, 56, 875; Salomon, R. et al. Proc. Natl. Acad. Sci. U.S.A. 2007, 104, 12479)

Monotherapy with a single antiviral drug for influenza may be limited in efficacy due to the rapidly developed drug-resistance. (Baz, M. et al. New Engl. J. Med. 2009, 361, 2296). Moreover, the uncontrolled virus-induced cytokines could cause the high mortality of human infected by H5N1 avian influenza virus.

In a different approach, combination chemotherapy consisting of two or more drugs that target different viral proteins or host immune response may provide additive or synergistic antiviral activities, (Govorkova, E. A. & Webster, R. G. Viruses 2010, 2, 1510) and thus reduce the risk of drug-resistance with monotherapy.

Zanamivir (ZA) is a potent influenza virus neuraminidase (NA) inhibitor, known as Relenza™ on market; (Ryan, D. M. et al. Antimicrob. Agents Chemother. 1994, 38, 2270) the viral resistance to zanamivir is rare. (Collins, P. J. et al. Nature 2008, 453, 1258-1261). Zanamivir combined with triamcinolone, an anti-inflammatory corticosteroid, has been applied to treat H3N2 virus infection. (Ottolini, M. et al. Pediatr. Pulmonol. 2003, 36, 290) In another study, (Zheng, B. J. et al. Proc. Natl. Acad. Sci. USA 2008, 105, 8091) the mice inflected with highly virulent H5N1 virus (1000 LD₅₀) receiving a triple combination of zanamivir with immuno-modulating drugs (celocoxib and mesalazine) showed better survival rate in comparison with the treatment with zanamivir alone. The enhanced therapeutic effect may be attributable to the synergistic antiviral activity and cytokine suppression.

Therefore, there is a need for newer and synergistically effective bifunctional drugs for the prophylaxis, treatment and therapy of influenza virus infection where the drugs are formed by conjugation of an anti-influenza moiety with a moiety that suppresses pro-inflammatory cytokines.

SUMMARY OF THE INVENTION

The present invention provides compounds with dual-targeted and bifunctional anti-influenza activities. The provided compounds are generally formed by conjugation one or more anti-inflammatory agents with one or more anti-influenza agents. Exemplary compounds according to the invention include caffeic acid (CA)-bearing zanamivir (ZA) conjugates ZA-7-CA (1), ZA-7-CA-amide (7) and ZA-7-Nap (43), for simultaneous inhibition of influenza virus neuraminidase and suppression of proinflammatory cytokines. Preparation of these enhanced anti-influenza conjugate compounds are described herein. The present application also demonstrates that the synthetic bifunctional ZA conjugates act synergistically towards protection of mice lethally infected by H1N1 or H5N1 influenza viruses. Unexpectedly, the efficacy of ZA-7-CA, ZA-7-CA-amide and ZA-7-Nap conjugates was found to be much greater than the combination therapy of ZA with anti-inflammatory agents.

In one aspect, the invention provides chimeric compounds derived from neuraminidase inhibitors and anti-inflammatory agents (see, e.g., those shown in FIG. 2), such as the conjugates 43-51.

In another aspect, the invention provides dual-targeted anti-influenza compounds by conjugating one or more anti-inflamatory agents with one ore more anti-inflammatory agents. In certain embodiments, the anti-inflamatory agent is zanamivir (ZA), phosphono-zanamivir (PZA), oseltamivir (OS), tamiphosphor (TP) or peramivir (PE). In certain embodiments, the anti-inflammatory agents are caffeic acid (CA), mesalazine (ME), naproxen (Nap) and ibuprofen (Ibu). In certain embodiments, the provided compounds simultaneously inhibit influenza virus neuraminidase and suppression of proinflammatory cytokines. These anti-influenza conjugates show synergistic effect to provide remarkable protection of cells and mice against influenza infections. Intranasal administration of low dosage (<1.2 μmol/kg/day) of zanamivir conjugates, in particular ZA-7-CA (1) and ZA-7-CA-amido (7) conjugates, showed greater effect than the combination therapy with ZA and anti-inflammatory agents in protection of the lethally infected mice by H1N1 or H5N1 influenza viruses.

In another aspect, the invention provides chimeric, bifunctional compounds comprising a first moiety and a second moiety. In certain embodiments, the first moiety is an anti-influenza agent. In certain embodiments, the second moiety is an anti-inflammatory agent or an antioxidant moiety. In certain embodiments, the first and second moieties are conjugated via a linker. In certain embodiments, the anti-influenza agent and the anti-inflammatory agent are conjugated via a linker as in Formula (A). In certain embodiments, the linker comprises 2-12 carbon atoms and/or 0-5 heteroatoms selected from N, O and S.

In some embodiments, more than one (e.g., 2) anti-influenza agents can be conjugated with one anti-inflammatory agent via the linker. An example is shown below.

In other embodiments, one anti-influenza agent can be conjugated with one or more anti-inflammatory agents via the linker. An example is shown below.

In yet other embodiments, multiple anti-influenza agents are conjugated with multiple anti-inflammatory agents via the linker. Below is an example.

In some embodiments, the anti-inflammatory agent is a non-steroidal anti-inflammatory agent. In particular embodiments, the non-steroidal anti-inflammatory agent is an acid anti-inflammatory agent. In some embodiments, the anti-influenza agent is a derivative of zanamivir (ZA), phosphono-zanamivir (PZA), oseltamivir (OS), tamiphosphor (TP), or peramivir (PE).

In another aspect, a compound of formula (A) is of formula (I) or formula (II):

or a pharmaceutically acceptable salt thereof, wherein X₁, L, Y, B, R¹, R², R³, R⁴, and R⁵ are as defined herein.

In another aspect, a provided compound is of formula (III) or (IV):

or a pharmaceutically acceptable salt thereof, wherein X₂, L, Y, B, R², R³, R⁴, and R⁶ are as defined herein.

In another aspect, a provided compound is of formula (V) or (VI):

or a pharmaceutically acceptable salt thereof, wherein X₁, L, Y, B, R², R⁴, and R⁶ are as defined herein.

In another aspect, a provided compound is of formula (VII) or (VIII):

or a pharmaceutically acceptable salt thereof, wherein Y, B, R¹, R², R³, R⁴, R⁵, K and R⁶ are as defined herein.

In another aspect, a provided compound is of formula (IX) or (X):

or a pharmaceutically acceptable salt thereof, wherein X₂, L, Y, B, and R⁴ are as defined herein.

In another aspect, a provided compound is of formula (XI) or (XII):

or a pharmaceutically acceptable salt thereof, wherein X₁, L, Y, B, R¹, and R⁴ are as defined herein.

In some embodiments, a provided compound is any one of the following formulae:

In some embodiments, a provided compound is selected from the group consisting of:

In some embodiments, a provided compound is selected from the group consisting of:

In some embodiments, a provided compound is selected from the group consisting of:

In some embodiments, a provided compound is selected from the group consisting of:

In some embodiments, a provided compound is selected from the group consisting of:

In another aspect, the present invention provides pharmaceutical compositions comprising a compound of Formula (A), or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, and optionally a pharmaceutically acceptable excipient. In certain embodiments, the pharmaceutical compositions described herein include a therapeutically effective amount of a compound of Formula (A), or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof. In certain embodiments, the provided pharmaceutical composition simultaneously inhibits influenza virus and inflammation.

In some embodiments, the pharmaceutical composition inhibits influenza virus neuraminidase. In some embodiments, the pharmaceutical composition inhibits pro-inflammatory cytokines. In some embodiments, wherein the pharmaceutical composition inhibits influenza virus neuraminidase and pro-inflammatory cytokines at levels greater than the inhibition observed by a composition comprising equivalent amounts of the unconjugated influenza virus inhibitor and the unconjugated anti-inflammatory agent.

In some embodiments, the compounds of Formula (A), or pharmaceutically acceptable salts thereof, bind both human and avian influenza virus strains. In some embodiments, the compounds of Formula (A), or pharmaceutically acceptable salts thereof, bind at least one of H1N1 and H5N1 strains of influenza virus.

In some embodiments, the compounds of Formula (A), or pharmaceutically acceptable salts thereof, bind the neuraminidase protein of the influenza virus. In some embodiments, the neuraminidase is wild-type. In some embodiments, the neuraminidase is oseltamivir-resistant.

In another aspect, the present invention provides methods of treating or preventing influenza infection by administering to a subject in need thereof a therapeutically effective amount of a compound according to any of Formula (A), or pharmaceutically acceptable salt thereof. In some embodiments, the administration is continued until symptoms of influenza infection are reduced, alleviated or terminated. Also within the scope of the present disclosure are pharmaceutical compositions for use in treating influenza infection, the composition comprising one or more of the compounds of Formula (A) as described herein and a pharmaceutically acceptable carrier, as well as uses of such compounds for manufacturing a medicament for use in treating influenza infection.

In another aspect, the present invention provides kits comprising a compound of Formula (A), or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a pharmaceutical composition thereof. The kits of the invention may include a single dose or multiple doses of a compound of Formula (A), or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or a pharmaceutical composition thereof. The provided kits may be useful for the treatment and/or prevention of influenza infection. In certain embodiments, the kits described herein further include instructions for administering the compound of Formula (A), or the pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, or the pharmaceutical composition thereof. The kits may also include packaging information describing the use or prescribing information for the subject or a health care professional. Such information may be required by a regulatory agency such as the U.S. Food and Drug Administration (FDA). The kit may also optionally include a device for administration of the compound or composition, for example, a dropper for ocular administration or a syringe for parenteral administration.

The details of certain embodiments of the invention are set forth herein. Other features, objects, and advantages of the invention will be apparent from the Detailed Description, Figures, Examples, and Claims.

DEFINITIONS Chemical Definitions

Definitions of specific functional groups and chemical terms are described in more detail below. The chemical elements are identified in accordance with the Periodic Table of the Elements, CAS version, Handbook of Chemistry and Physics, 75^(th) Ed., inside cover, and specific functional groups are generally defined as described therein. Additionally, general principles of organic chemistry, as well as specific functional moieties and reactivity, are described in Organic Chemistry, Thomas Sorrell, University Science Books, Sausalito, 1999; Smith and March March's Advanced Organic Chemistry, 5^(th) Edition, John Wiley & Sons, Inc., New York, 2001; Larock, Comprehensive Organic Transformations, VCH Publishers, Inc., New York, 1989; and Carruthers, Some Modern Methods of Organic Synthesis, 3^(rd) Edition, Cambridge University Press, Cambridge, 1987.

Compounds described herein can comprise one or more asymmetric centers, and thus can exist in various stereoisomeric forms, e.g., enantiomers and/or diastereomers. For example, the compounds described herein can be in the form of an individual enantiomer, diastereomer or geometric isomer, or can be in the form of a mixture of stereoisomers, including racemic mixtures and mixtures enriched in one or more stereoisomer. Isomers can be isolated from mixtures by methods known to those skilled in the art, including chiral high pressure liquid chromatography (HPLC) and the formation and crystallization of chiral salts; or preferred isomers can be prepared by asymmetric syntheses. See, for example, Jacques et al., Enantiomers, Racemates and Resolutions (Wiley Interscience, New York, 1981); Wilen et al., Tetrahedron 33:2725 (1977); Eliel, E. L. Stereochemistry of Carbon Compounds (McGraw-Hill, NY, 1962); and Wilen, S. H. Tables of Resolving Agents and Optical Resolutions p. 268 (E. L. Eliel, Ed., Univ. of Notre Dame Press, Notre Dame, Ind. 1972). The invention additionally encompasses compounds as individual isomers substantially free of other isomers, and alternatively, as mixtures of various isomers.

When a range of values is listed, it is intended to encompass each value and sub-range within the range. For example “C₁₋₆ alkyl” is intended to encompass, C₁, C₂, C₃, C₄, C₅, C₆, C₁₋₆, C₁₋₅, C₁₋₄, C₁₋₃, C₁₋₂, C₂₋₆, C₂₋₅, C₂₋₄, C₂₋₃, C₃₋₆, C₃₋₅, C₃₋₄, C₄₋₆, C₄₋₅, and C₅₋₆ alkyl.

The term “aliphatic,” as used herein, refers to alkyl, alkenyl, alkynyl, and carbocyclic groups. Likewise, the term “heteroaliphatic” as used herein, refers to heteroalkyl, heteroalkenyl, heteroalkynyl, and heterocyclic groups.

As used herein, “alkyl” refers to a radical of a straight-chain or branched saturated hydrocarbon group having from 1 to 10 carbon atoms (“C₁₋₁₀ alkyl”). In some embodiments, an alkyl group has 1 to 9 carbon atoms (“C₁₋₉ alkyl”). In some embodiments, an alkyl group has 1 to 8 carbon atoms (“C₁₋₈ alkyl”). In some embodiments, an alkyl group has 1 to 7 carbon atoms (“C₁₋₇ alkyl”). In some embodiments, an alkyl group has 1 to 6 carbon atoms (“C₁₋₆ alkyl”). In some embodiments, an alkyl group has 1 to 5 carbon atoms (“C₁₋₅ alkyl”). In some embodiments, an alkyl group has 1 to 4 carbon atoms (“C₁₋₄ alkyl”). In some embodiments, an alkyl group has 1 to 3 carbon atoms (“C₁₋₃ alkyl”). In some embodiments, an alkyl group has 1 to 2 carbon atoms (“C₁₋₂ alkyl”). In some embodiments, an alkyl group has 1 carbon atom (“C₁ alkyl”). In some embodiments, an alkyl group has 2 to 6 carbon atoms (“C₂₋₆ alkyl”). Examples of C₁₋₆ alkyl groups include methyl (C₁), ethyl (C₂), n-propyl (C₃), isopropyl (C₃), n-butyl (C₄), tert-butyl (C₄), sec-butyl (C₄), iso-butyl (C₄), n-pentyl (C₅), 3-pentanyl (C₅), amyl (C₅), neopentyl (C₅), 3-methyl-2-butanyl (C₅), tertiary amyl (C₅), and n-hexyl (C₆). Additional examples of alkyl groups include n-heptyl (C₇), n-octyl (C₈) and the like. Unless otherwise specified, each instance of an alkyl group is independently unsubstituted (an “unsubstituted alkyl”) or substituted (a “substituted alkyl”) with one or more substituents. In certain embodiments, the alkyl group is an unsubstituted C₁₋₁₀ alkyl (e.g., CH₃). In certain embodiments, the alkyl group is a substituted C₁₋₁₀ alkyl.

As used herein, “haloalkyl” is a substituted alkyl group as defined herein wherein one or more of the hydrogen atoms are independently replaced by a halogen, e.g., fluoro, bromo, chloro, or iodo. “Perhaloalkyl” is a subset of haloalkyl, and refers to an alkyl group wherein all of the hydrogen atoms are independently replaced by a halogen, e.g., fluoro, bromo, chloro, or iodo. In some embodiments, the haloalkyl moiety has 1 to 8 carbon atoms (“C₁₋₈ haloalkyl”). In some embodiments, the haloalkyl moiety has 1 to 6 carbon atoms (“C₁₋₆ haloalkyl”). In some embodiments, the haloalkyl moiety has 1 to 4 carbon atoms (“C₁₋₄ haloalkyl”). In some embodiments, the haloalkyl moiety has 1 to 3 carbon atoms (“C₁₋₃ haloalkyl”). In some embodiments, the haloalkyl moiety has 1 to 2 carbon atoms (“C₁₋₂ haloalkyl”). In some embodiments, all of the haloalkyl hydrogen atoms are replaced with fluoro to provide a perfluoroalkyl group. In some embodiments, all of the haloalkyl hydrogen atoms are replaced with chloro to provide a “perchloroalkyl” group. Examples of haloalkyl groups include CF₃, CF₂CF₃, CF₂CF₂CF₃, CCl₃, CFCl₂, CF₂Cl, and the like.

As understood from the above, alkyl, alkenyl, alkynyl, heteroalkyl, heteroalkenyl, heteroalkynyl, carbocyclyl, heterocyclyl, aryl, and heteroaryl groups, as defined herein, are, in certain embodiments, optionally substituted. Optionally substituted refers to a group which may be substituted or unsubstituted (e.g., “substituted” or “unsubstituted” alkyl, “substituted” or “unsubstituted” alkenyl, “substituted” or “unsubstituted” alkynyl, “substituted” or “unsubstituted” heteroalkyl, “substituted” or “unsubstituted” heteroalkenyl, “substituted” or “unsubstituted” heteroalkynyl, “substituted” or “unsubstituted” carbocyclyl, “substituted” or “unsubstituted” heterocyclyl, “substituted” or “unsubstituted” aryl or “substituted” or “unsubstituted” heteroaryl group). In general, the term “substituted” means that at least one hydrogen present on a group is replaced with a permissible substituent, e.g., a substituent which upon substitution results in a stable compound, e.g., a compound which does not spontaneously undergo transformation such as by rearrangement, cyclization, elimination, or other reaction. Unless otherwise indicated, a “substituted” group has a substituent at one or more substitutable positions of the group, and when more than one position in any given structure is substituted, the substituent is either the same or different at each position. The term “substituted” is contemplated to include substitution with all permissible substituents of organic compounds, any of the substituents described herein that results in the formation of a stable compound. The present invention contemplates any and all such combinations in order to arrive at a stable compound. For purposes of this invention, heteroatoms such as nitrogen may have hydrogen substituents and/or any suitable substituent as described herein which satisfy the valencies of the heteroatoms and results in the formation of a stable moiety.

As used herein, the term “halo” or “halogen” refers to fluorine (fluoro, —F), chlorine (chloro, —Cl), bromine (bromo, —Br), or iodine (iodo, —I).

As used herein, a “counterion” is a negatively charged group associated with a positively charged quarternary amine in order to maintain electronic neutrality. Exemplary counterions include halide ions (e.g., F⁻, Cl⁻, Br⁻, I⁻), NO₃ ⁻, ClO₄ ⁻, OH⁻, H₂PO₄ ⁻, HSO₄ ⁻, sulfonate ions (e.g., methansulfonate, trifluoromethanesulfonate, p-toluenesulfonate, benzenesulfonate, 10-camphor sulfonate, naphthalene-2-sulfonate, naphthalenelsulfonic acid-5-sulfonate, ethanlsulfonic acid-2-sulfonate, and the like), and carboxylate ions (e.g., acetate, ethanoate, propanoate, benzoate, glycerate, lactate, tartrate, glycolate, and the like).

As used herein, the term “hydroxyl” or “hydroxy” refers to the group —OH. The term “substituted hydroxyl” or “substituted hydroxyl,” by extension, refers to a hydroxyl group wherein the oxygen atom directly attached to the parent molecule is substituted with a group other than hydrogen, and includes groups selected from —OR^(aa), —ON(R^(bb))₂, —OC(═O)SR^(aa), —OC(═O)R^(aa), —OCO₂R^(aa), —OC(═O)N(R^(bb))₂, —OC(═NR^(bb))R^(aa), —OC(═NR^(bb))OR^(aa), —OC(═NR^(bb))N(R^(bb))₂, —OS(═O)R^(aa), —OSO₂R^(aa), —OSi(R^(aa))₃, —OP(R^(cc))₂, —OP(R^(cc))₃, —OP(═O)₂R^(aa), —OP(═O)(R^(aa))₂, —OP(═O)(OR^(cc))₂, OP(═O)₂N(R^(bb))₂, and —OP(═O)(NR^(bb))₂, wherein R^(aa), R^(bb), and R^(cc) are as defined herein.

As used herein, the term, “amino” refers to the group —NH₂. The term “substituted amino,” by extension, refers to a monosubstituted amino, a disubstituted amino, or a trisubstituted amino, as defined herein. In certain embodiments, the “substituted amino” is a monosubstituted amino or a disubstituted amino group.

As used herein, the term “monosubstituted amino” refers to an amino group wherein the nitrogen atom directly attached to the parent molecule is substituted with one hydrogen and one group other than hydrogen, and includes groups selected from NH(R^(bb)), NHC(═O)R^(aa), —NHCO₂R^(aa), NHC(═O)N(R^(bb))₂, NHC(═NR^(bb))N(R^(bb))₂, NHSO₂R^(aa), NHP(═O)(OR^(cc))₂, and —NHP(═O)(NR^(bb))₂, wherein R^(aa), R^(bb) and R^(cc) are as defined herein, and wherein R^(bb) of the group NH(R^(bb)) is not hydrogen.

As used herein, the term “disubstituted amino” refers to an amino group wherein the nitrogen atom directly attached to the parent molecule is substituted with two groups other than hydrogen, and includes groups selected from —N(R⁶⁶)₂, —NR^(bb)C(═O)R^(aa), —NR^(bb)CO₂R^(aa), —NR^(bb)C(═O)N(R^(bb))₂, —NR^(bb)C(═NR^(bb))N(R^(bb))₂, —NR^(bb)SO₂R^(aa), —NR^(bb)P(═O)(OR^(cc))₂, and —NR^(bb)P(═O)(NR^(bb))₂, wherein R^(aa), R^(bb), and R^(cc) are as defined herein, with the proviso that the nitrogen atom directly attached to the parent molecule is not substituted with hydrogen.

As used herein, the term “trisubstituted amino” refers to an amino group wherein the nitrogen atom directly attached to the parent molecule is substituted with three groups, and includes groups selected from —N(R^(bb))₃ and —N(R^(bb))₃ ⁺X⁻, wherein R^(bb) and X⁻ are as defined herein.

As used herein, the term “hydroxyl” or “hydroxy” refers to the group —OH. The term “substituted hydroxyl” or “substituted hydroxyl,” by extension, refers to a hydroxyl group wherein the oxygen atom directly attached to the parent molecule is substituted with a group other than hydrogen, and includes groups selected from —OR^(aa), —ON(R^(bb))₂—OC(═O)SR^(aa), —OC(═O)R^(aa), —OCO₂R^(aa), —OC(═O)N(R^(bb))₂, —OC(═NR^(bb))R^(aa), —OC(═NR^(bb))OR^(aa), —OC(═NR^(bb))N(R^(bb))₂, —OS(═O)R^(aa), —OSO₂R^(aa), —OSi(R^(aa))₃, —PP(R^(cc))₂, —OP(R^(cc))₃, —OP(═O)₂R^(aa), —OP(═O)(R^(aa))₂, —OP(═O)(OR^(cc))₂, —OP(═O)₂N(R^(bb))₂, and —OP(═O)(NR^(bb))₂, wherein R^(aa), R^(bb), and R^(cc) are as defined herein. In the case wherein “substituted hydroxyl” is a ligand L₁ or L₂, “substituted hydroxyl” also refers to the group (R^(aa))₂O, wherein R^(aa) is as defined herein.

Nitrogen protecting groups such as carbamate groups (e.g., —C(═O)OR^(aa)) include, but are not limited to, methyl carbamate, ethyl carbamante, 9-fluorenylmethyl carbamate (Fmoc), 9-(2-sulfo)fluorenylmethyl carbamate, 9-(2,7-dibromo)fluoroenylmethyl carbamate, 2,7-di-t-butyl-[9-(10,10-dioxo-10,10,10,10-tetrahydrothioxanthyl)]methyl carbamate (DBDT-moc), 4-methoxyphenacyl carbamate (Phenoc), 2,2,2-trichloroethyl carbamate (Troc), 2-trimethylsilylethyl carbamate (Teoc), 2-phenylethyl carbamate (hZ), 1-(1-adamantyl)-1-methylethyl carbamate (Adpoc), 1,1-dimethyl-2-haloethyl carbamate, 1,1-dimethyl-2,2-dibromoethyl carbamate (DB-t-BOC), 1,1-dimethyl-2,2,2-trichloroethyl carbamate (TCBOC), 1-methyl-1-(4-biphenylyl)-ethyl carbamate (Bpoc), 1-(3,5-di-t-butylphenyl)-1-methylethyl carbamate (t-Bumeoc), 2-(2′- and 4′-pyridyl)ethyl carbamate (Pyoc), 2-(N,N-dicyclohexylcarboxamido)ethyl carbamate, t-butyl carbamate (BOC), 1-adamantyl carbamate (Adoc), vinyl carbamate (Voc), allyl carbamate (Alloc), 1-isopropylallyl carbamate (Ipaoc), cinnamyl carbamate (Coc), 4-nitrocinnamyl carbamate (Noc), 8-quinolyl carbamate, N-hydroxypiperidinyl carbamate, alkyldithio carbamate, benzyl carbamate (Cbz), p-methoxybenzyl carbamate (Moz), pnitobenzyl carbamate, pbromobenzyl carbamate, p-chlorobenzyl carbamate, 2,4-dichlorobenzyl carbamate, 4-methylsulfinylbenzyl carbamate (Msz), 9-anthrylmethyl carbamate, diphenylmethyl carbamate, 2-methylthioethyl carbamate, 2-methylsulfonylethyl carbamate, 2-(p-toluenesulfonyl)ethyl carbamate, [2-(1,3-dithianyl)]methyl carbamate (Dmoc), 4-methylthiophenyl carbamate (Mtpc), 2,4-dimethylthiophenyl carbamate (Bmpc), 2-phosphonioethyl carbamate (Peoc), 2-triphenylphosphonioisopropyl carbamate (Ppoc), 1,1-dimethyl-2-cyanoethyl carbamate, m-chloro-p-acyloxybenzyl carbamate, p-(dihydroxyboryl)benzyl carbamate, 5-benzisoxazolylmethyl carbamate, 2-(trifluoromethyl)-6-chromonylmethyl carbamate (Tcroc), m-nitrophenyl carbamate, 3,5-dimethoxybenzyl carbamate, o-nitrobenzyl carbamate, 3,4-dimethoxy-6-nitrobenzyl carbamate, phenyl(o-nitrophenyl)methyl carbamate, t-amyl carbamate, S-benzyl thiocarbamate, p-cyanobenzyl carbamate, cyclobutyl carbamate, cyclohexyl carbamate, cyclopentyl carbamate, cyclopropylmethyl carbamate, p-decyloxybenzyl carbamate, 2,2-dimethoxyacylvinyl carbamate, o-(N,N-dimethylcarboxamido)benzyl carbamate, 1,1-dimethyl-3-(N,N-dimethylcarboxamido)propyl carbamate, 1,1-dimethylpropynyl carbamate, di(2-pyridyl)methyl carbamate, 2-furanylmethyl carbamate, 2-iodoethyl carbamate, isoborynl carbamate, isobutyl carbamate, isonicotinyl carbamate, p-(p′-methoxyphenylazo)benzyl carbamate, 1-methylcyclobutyl carbamate, 1-methylcyclohexyl carbamate, 1-methyl-1-cyclopropylmethyl carbamate, 1-methyl-1-(3,5-dimethoxyphenyl)ethyl carbamate, 1-methyl-1-(p-phenylazophenyl)ethyl carbamate, 1-methyl-1-phenylethyl carbamate, 1-methyl-1-(4-pyridyl)ethyl carbamate, phenyl carbamate, p-(phenylazo)benzyl carbamate, 2,4,6-tri-t-butylphenyl carbamate, 4-(trimethylammonium)benzyl carbamate, and 2,4,6-trimethylbenzyl carbamate.

Nitrogen protecting groups such as sulfonamide groups (e.g., S(═O)₂R^(aa)) include, but are not limited to, p-toluenesulfonamide (Ts), benzenesulfonamide, 2,3,6,trimethyl-4-methoxybenzenesulfonamide (Mtr), 2,4,6-trimethoxybenzenesulfonamide (Mtb), 2,6-dimethyl 4-methoxybenzenesulfonamide (Pme), 2,3,5,6-tetramethyl-4-methoxybenzenesulfonamide (Mte), 4-methoxybenzenesulfonamide (Mbs), 2,4,6-trimethylbenzenesulfonamide (Mts), 2,6-dimethoxy-4-methylbenzenesulfonamide (iMds), 2,2,5,7,8-pentamethylchroman-6-sulfonamide (Pmc), methanesulfonamide (Ms), β-trimethylsilylethanesulfonamide (SES), 9-anthracenesulfonamide, 4-(4′,8′-dimethoxynaphthylmethyl)benzenesulfonamide (DNMBS), benzylsulfonamide, trifluoromethylsulfonamide, and phenacylsulfonamide.

Other nitrogen protecting groups include, but are not limited to, phenothiazinyl(10) acyl derivative, N′-p-toluenesulfonylaminoacyl derivative, N′-phenylaminothioacyl derivative, N-benzoylphenylalanyl derivative, N-acetylmethionine derivative, 4,5-diphenyl-3-oxazolin-2-one, N-phthalimide, N-dithiasuccinimide (Dts), N-2,3-diphenylmaleimide, N-2,5-dimethylpyrrole, N-1,1,4,4-tetramethyldisilylazacyclopentane adduct (STAB ASE), 5-substituted 1,3-dimethyl-1,3,5-triazacyclohexan-2-one, 5-substituted 1,3-dibenzyl-1,3,5-triazacyclohexan-2-one, 1-substituted 3,5-dinitro-4-pyridone, N-methylamine, N-allylamine, N-[2-(trimethylsilyl)ethoxy]methylamine (SEM), N-3-acetoxypropylamine, N-(1-isopropyl-4-nitro-2-oxo-3-pyroolin-3-yl)amine, quaternary ammonium salts, N-benzylamine, N-di(4-methoxyphenyl)methylamine, N-5-dibenzosuberylamine, N-triphenylmethylamine (Tr), N-[(4-methoxyphenyl)diphenylmethyl]amine (MMTr), N-9-phenylfluorenylamine (PhF), N-2,7-dichloro-9-fluorenylmethyleneamine, N-ferrocenylmethylamino (Fcm), N-2 picolylamino N′-oxide, N-1,1-dimethylthiomethyleneamine, N-benzylideneamine, N-p-methoxybenzylideneamine, N-diphenylmethyleneamine, N-[2-pyridyl)mesityl]methyleneamine, N-(N′,N′-dimethylaminomethylene)amine, N,N′-isopropylidenediamine, N-p-nitrobenzylideneamine, N-salicylideneamine, N-5-chlorosalicylideneamine, N-(5-chloro-2-hydroxyphenyl)phenylmethyleneamine, N-cyclohexylideneamine, N-(5,5-dimethyl-3-oxo-1-cyclohexenyl)amine, N-borane derivative, N-diphenylborinic acid derivative, N-[phenyl(pentaacylchromium- or tungsten)acyl]amine, N-copper chelate, N-zinc chelate, N-nitroamine, N-nitrosoamine, amine N-oxide, diphenylphosphinamide (Dpp), dimethylthiophosphinamide (Mpt), diphenylthiophosphinamide (Ppt), dialkyl phosphoramidates, dibenzyl phosphoramidate, diphenyl phosphoramidate, benzenesulfenamide, onitrobenzenesulfenamide (Nps), 2,4-dinitrobenzenesulfenamide, pentachlorobenzenesulfenamide, 2-nitro-4-methoxybenzenesulfenamide, triphenylmethylsulfenamide, and 3-nitropyridinesulfenamide (Npys).

These and other exemplary substituents are described in more detail in the Detailed Description, Examples, and Claims. The invention is not intended to be limited in any manner by the above exemplary listing of substituents.

As used herein, the term “salt” refers to any and all salts.

The term “pharmaceutically acceptable salt” refers to those salts which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response and the like, and are commensurate with a reasonable benefit/risk ratio. Pharmaceutically acceptable salts are well known in the art. For example, Berge et al. describes pharmaceutically acceptable salts in detail in J. Pharmaceutical Sciences (1977) 66:1-19. Pharmaceutically acceptable salts of the compounds of this invention include those derived from suitable inorganic and organic acids and bases. Examples of pharmaceutically acceptable, nontoxic acid addition salts are salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid or with organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid, or malonic acid or by using other methods used in the art such as ion exchange. Other pharmaceutically acceptable salts include adipate, alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, formate, fumarate, glucoheptonate, glycerophosphate, gluconate, hemisulfate, heptanoate, hexanoate, hydroiodide, 2-hydroxyethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, pectinate, persulfate, 3-phenylpropionate, phosphate, picrate, pivalate, propionate, stearate, succinate, sulfate, tartrate, thiocyanate, p-toluenesulfonate, undecanoate, valerate salts, and the like. Pharmaceutically acceptable salts derived from appropriate bases include alkali metal, alkaline earth metal, ammonium and N⁺(C₁₋₄alkyl)₄ salts. Representative alkali or alkaline earth metal salts include sodium, lithium, potassium, calcium, magnesium, and the like. Further pharmaceutically acceptable salts include, when appropriate, nontoxic ammonium, quaternary ammonium, and amine cations formed using counterions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, lower alkyl sulfonate, and aryl sulfonate.

As used herein, the term “tautomer” refers to particular isomers of a compound in which a hydrogen and double bond have changed position with respect to the other atoms of the molecule. For a pair of tautomers to exist there must be a mechanism for interconversion. Examples of tautomers include keto-enol forms, imine-enamine forms, amide-imino alcohol forms, amidine-aminidine forms, nitroso-oxime forms, thio ketone-enethiol forms, N-nitroso-hydroxyazo forms, nitro-aci-nitro forms, and pyridone-hydroxypyridine forms.

As used herein, use of the phrase “at least one instance” refers to 1, 2, 3, 4, or more instances, but also encompasses a range, e.g., for example, from 1 to 4, from 1 to 3, from 1 to 2, from 2 to 4, from 2 to 3, or from 3 to 4 instances, inclusive.

Other Definition

The following definitions are more general terms used throughout the present application:

As used herein, the term “pharmaceutically acceptable salt” refers to those salts which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response and the like, and are commensurate with a reasonable benefit/risk ratio. Pharmaceutically acceptable salts are well known in the art. For example, Berge et al., describe pharmaceutically acceptable salts in detail in J. Pharmaceutical Sciences, 1977, 66, 1-19, incorporated herein by reference. Pharmaceutically acceptable salts of the compounds of this invention include those derived from suitable inorganic and organic acids and bases. Examples of pharmaceutically acceptable, nontoxic acid addition salts are salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid, and perchloric acid or with organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid, or malonic acid or by using other methods known in the art such as ion exchange. Other pharmaceutically acceptable salts include adipate, alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, formate, fumarate, glucoheptonate, glycerophosphate, gluconate, hemisulfate, heptanoate, hexanoate, hydroiodide, 2-hydroxy-ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, pectinate, persulfate, 3-phenylpropionate, phosphate, picrate, pivalate, propionate, stearate, succinate, sulfate, tartrate, thiocyanate, p-toluenesulfonate, undecanoate, valerate salts, and the like. Salts derived from appropriate bases include alkali metal, alkaline earth metal, ammonium and N⁺(C₁₋₄ alkyl)₄ salts. Representative alkali or alkaline earth metal salts include sodium, lithium, potassium, calcium, magnesium, and the like. Further pharmaceutically acceptable salts include, when appropriate, non-toxic ammonium, quaternary ammonium, and amine cations formed using counterions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, loweralkyl sulfonate, and aryl sulfonate.

The term “solvate” refers to forms of the compound that are associated with a solvent, usually by a solvolysis reaction. This physical association may include hydrogen bonding. Conventional solvents include water, methanol, ethanol, acetic acid, DMSO, THF, diethyl ether, and the like. The compounds of Formulae (I)-(II) may be prepared, e.g., in crystalline form, and may be solvated. Suitable solvates include pharmaceutically acceptable solvates and further include both stoichiometric solvates and non-stoichiometric solvates. In certain instances, the solvate will be capable of isolation, for example, when one or more solvent molecules are incorporated in the crystal lattice of a crystalline solid. “Solvate” encompasses both solution-phase and isolable solvates. Representative solvates include hydrates, ethanolates, and methanolates.

The term “hydrate” refers to a compound which is associated with water. Typically, the number of the water molecules contained in a hydrate of a compound is in a definite ratio to the number of the compound molecules in the hydrate. Therefore, a hydrate of a compound may be represented, for example, by the general formula R·x H₂O, wherein R is the compound, and x is a number greater than 0. A given compound may form more than one type of hydrates, including, e.g., monohydrates (x is 1), lower hydrates (x is a number greater than 0 and smaller than 1, e.g., hemihydrates (R·0.5 H₂O)), and polyhydrates (x is a number greater than 1, e.g., dihydrates (R·2H₂O) and hexahydrates (R·6H₂O)).

As used herein, the term “tautomer” includes two or more interconvertable forms resulting from at least one formal migration of a hydrogen atom and at least one change in valency (e.g., a single bond to a double bond, a triple bond to a double bond, or vice versa). The exact ratio of the tautomers depends on several factors, including temperature, solvent, and pH. Tautomerizations (i.e., the reaction providing a tautomeric pair) may be catalyzed by acid or base. Exemplary tautomerizations include keto-to-enol; amide-to-imide; lactam-to-lactim; enamine-to-imine; and enamine-to-(a different) enamine tautomerizations.

It is also to be understood that compounds that have the same molecular formula but differ in the nature or sequence of bonding of their atoms or the arrangement of their atoms in space are termed “isomers”. Isomers that differ in the arrangement of their atoms in space are termed “stereoisomers”.

Stereoisomers that are not mirror images of one another are termed “diastereomers” and those that are non-superimposable mirror images of each other are termed “enantiomers”. When a compound has an asymmetric center, for example, it is bonded to four different groups, a pair of enantiomers is possible. An enantiomer can be characterized by the absolute configuration of its asymmetric center and is described by the R- and S-sequencing rules of Cahn and Prelog, or by the manner in which the molecule rotates the plane of polarized light and designated as dextrorotatory or levorotatory (i.e., as (+) or (−)-isomers respectively). A chiral compound can exist as either individual enantiomer or as a mixture thereof. A mixture containing equal proportions of the enantiomers is called a “racemic mixture”.

The term “polymorphs” refers to a crystalline form of a compound (or a salt, hydrate, or solvate thereof) in a particular crystal packing arrangement. All polymorphs have the same elemental composition. Different crystalline forms usually have different X-ray diffraction patterns, infrared spectra, melting points, density, hardness, crystal shape, optical and electrical properties, stability, and/or solubility. Recrystallization solvent, rate of crystallization, storage temperature, and other factors may cause one crystal form to dominate. Various polymorphs of a compound can be prepared by crystallization under different conditions.

The term “prodrugs” refer to compounds, including derivatives of the compounds of Formula (I), which have cleavable groups and become by solvolysis or under physiological conditions the compounds of Formula (I)-(II) which are pharmaceutically active in vivo. Such examples include, but are not limited to, choline ester derivatives and the like, N-alkylmorpholine esters and the like. Other derivatives of the compounds of this invention have activity in both their acid and acid derivative forms, but in the acid sensitive form often offers advantages of solubility, tissue compatibility, or delayed release in the mammalian organism (see, Bundgard, Design of Prodrugs, pp. 7-9, 21-24, Elsevier, Amsterdam 1985). Prodrugs include acid derivatives well known to practitioners of the art, such as, for example, esters prepared by reaction of the parent acid with a suitable alcohol, or amides prepared by reaction of the parent acid compound with a substituted or unsubstituted amine, or acid anhydrides, or mixed anhydrides. Simple aliphatic or aromatic esters, amides, and anhydrides derived from acidic groups pendant on the compounds of this invention are particular prodrugs. In some cases it is desirable to prepare double ester type prodrugs such as (acyloxy)alkyl esters or ((alkoxycarbonyl)oxy)alkylesters. C₁-C₈ alkyl, C₂-C₈ alkenyl, C₂-C₈ alkynyl, aryl, C₇-C₁₂ substituted aryl, and C₇-C₁₂ arylalkyl esters of the compounds of Formulae (I)-(II) may be preferred in certain instances.

A “subject” to which administration is contemplated includes, but is not limited to, humans (i.e., a male or female of any age group, e.g., a pediatric subject (e.g., infant, child, adolescent) or adult subject (e.g., young adult, middle-aged adult, or senior adult)) and/or other nonhuman animals, for example, mammals (e.g., primates (e.g., cynomolgus monkeys, rhesus monkeys); commercially relevant mammals such as cattle, pigs, horses, sheep, goats, cats, and/or dogs) and birds (e.g., commercially relevant birds such as chickens, ducks, geese, and/or turkeys). In certain embodiments, the animal is a mammal. The animal may be a male or female and at any stage of development. A nonhuman animal may be a transgenic animal.

The terms “administer,” “administering,” or “administration,” as used herein refers to implanting, absorbing, ingesting, injecting, inhaling, or otherwise introducing an inventive compound, or a pharmaceutical composition thereof.

As used herein, the terms “treatment,” “treat,” and “treating” refer to reversing, alleviating, delaying the onset of, or inhibiting the progress of a “pathological condition” (e.g., a disease, disorder, or condition, or one or more signs or symptoms thereof) described herein. In some embodiments, treatment may be administered after one or more signs or symptoms have developed or have been observed. In other embodiments, treatment may be administered in the absence of signs or symptoms of the disease or condition. For example, treatment may be administered to a susceptible individual prior to the onset of symptoms (e.g., in light of a history of symptoms and/or in light of genetic or other susceptibility factors). Treatment may also be continued after symptoms have resolved, for example, to delay or prevent recurrence, or to reduce the risk for the disease or condition.

As used herein, the terms “condition,” “disease,” and “disorder” are used interchangeably.

An “effective amount” of a compound of Formulae (A) refers to an amount sufficient to elicit a desired biological response, i.e., treating the condition. As will be appreciated by those of ordinary skill in this art, the effective amount of a compound of Formula (A) may vary depending on such factors as the desired biological endpoint, the pharmacokinetics of the compound, the condition being treated, the mode of administration, and the age and health of the subject. An effective amount encompasses therapeutic and prophylactic treatment. For example, in treating cancer, an effective amount of an inventive compound may reduce the tumor burden or stop the growth or spread of a tumor. In treating macular degeneration, an effective amount of an inventive compound may improve sight, reduce the risk of vision loss, or prevent central vision loss from worsening.

A “therapeutically effective amount” of a compound of Formula (A) is an amount sufficient to provide a therapeutic benefit in the treatment of a condition or to delay or minimize one or more symptoms associated with the condition. A therapeutically effective amount of a compound means an amount of therapeutic agent, alone or in combination with other therapies, which provides a therapeutic benefit in the treatment of the condition. The term “therapeutically effective amount” can encompass an amount that improves overall therapy, reduces or avoids symptoms or causes of the condition, or enhances the therapeutic efficacy of another therapeutic agent.

A “prophylactically effective amount” of a compound of Formula (A) is an amount sufficient to prevent a condition, or one or more symptoms associated with the condition or prevent its recurrence. A prophylactically effective amount of a compound means an amount of a therapeutic agent, alone or in combination with other agents, which provides a prophylactic benefit in the prevention of the condition. The term “prophylactically effective amount” can encompass an amount that improves overall prophylaxis or enhances the prophylactic efficacy of another prophylactic agent.

As used herein “inhibition”, “inhibiting”, “inhibit” and “inhibitor”, and the like, refer to the ability of a compound to reduce, slow, halt, or prevent the activity of a particular biological process involving Ras in a cell relative to vehicle.

BRIEF DESCRIPTION OF THE DRAWINGS

The following drawings form part of the present specification and are included to further demonstrate certain aspects of the present disclosure, the inventions of which can be better understood by reference to one or more of these drawings in combination with the detailed description of specific embodiments presented herein.

FIG. 1. Working mechanism of the dual-targeted anti-influenza drugs for virus inactivation and cytokine suppression. The conjugate drug is brought to the virus infection sites via strong affinity of the neuraminidase inhibitor (e.g., zanamivir), so that the infection-mediated cytokine induction can be effectively suppressed by the anti-inflammatory component (e.g., caffeic acid) of the conjugate drug.

FIG. 2. Chemical structures of exemplary anti-influenza conjugate drugs

FIG. 3. Synthetic design for the exemplary conjugate compounds of zanamivir with anti-inflammatory agent at 1-, 7-, 9- or guanidine (G)-positions.

FIG. 4. Synthesis of ZA-7-HP conjugate 6

FIG. 5. Synthesis of ZA-7-CA conjugate 1

FIG. 6. Synthesis of ZA-7-MCA conjugate 2

FIG. 7. Synthesis of ZA-7-ME conjugate 3

FIG. 8. Synthesis of ZA-7-HNAP conjugate 4

FIG. 9. Synthesis of ZA-7-DHBA conjugate 5

FIG. 10. Synthesis of ZA-7-CA-amido conjugate 7

FIG. 11. Synthesis of ZA-7-HNAP-amido conjugate 8

FIG. 12. Synthesis of ZA-1-CA conjugate 9

FIG. 13. Synthesis of ZA-1-MCA conjugate 10

FIG. 14. Synthesis of ZA-1-ME conjugate 11

FIG. 15. Synthesis of ZA-1-HNAP conjugate 12

FIG. 16. Survival rates of H1N1 virus-infected mice on treatment with ZA-7-CA (1), ZA-7-ME (3) and ZA-7-CA-amide (7) conjugates. BALB/c mice were intranasally challenged with 10 LD₅₀ of A/Cal/07/2009 (H1N1) viruses. The animal experiments were conducted with at least 10 mice per group. Compounds were administered by intranasal instillation and continued twice daily for 5 days. The administered dosages of each compound are shown in parentheses in unit of μmol/kg/day if the human-equivalent dose (1.2 μmol/kg/day) is not used. (A) 12 μmol/kg/day dosage; (B) 1.2 μmol/kg/day dosage; (C) various dosages; (D) various dosages. **: p<0.05; ***: p<0.001; ****: p<0.0001.

FIG. 17. Survival rates of H5N1 virus-infected mice on treatment with ZA-7-CA (1), ZA-7-ME (3) and ZA-7-CA-amide (7) conjugates. BALB/c mice were intranasally challenged with 10 LD₅₀ of NIBRG-14 (A/VietNam/1194/2004) (H5N1) viruses. The animal experiments were conducted with 10 mice per group. Compounds were administered by intranasal instillation and continued twice daily for 5 days. (A) 12 μmol/kg/day dosage; and (B) 12 or 1.2 μmol/kg/day dosage. **: p<0.05; ***: p<0.001; ****: p<0.0001.

FIG. 18. Cytokine suppression in LPS-induced inflammation assay and in virus-infected mice. (A) RAW 264.7 cells were treated with different concentrations of anti-inflammatory drugs for 30 min before induction with lipopolysaccharide (LPS, 100 ng/mL): lane 1, negative control (no LPS); lane 2, no compound treatment; lane 3, apigenin (20 μM); lane 4, apigenin (50 μM) as a positive control; lane 5, CA (20 μM); lane 6, CA (50 μM); lane 7, conjugate 2 (20 μM); lane 8, conjugate 2 (50 μM); lane 9, conjugate 1 (20 μM); lane 10, conjugate 1 (50 μM); lane 11, MCA (20 μM); lane 12, MCA (50 μM); lane 13, ME (20 μM); and lane 14, ME (50 μM). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is used as an internal control. (B) The levels of cytokine IL-6 were detected in the sera collected at 72 h after the mice (n=10) were challenged by 10 LD₅₀ NIBRG14 (A/VietNam/1194/2004) H5N1 viruses, and then treated with test compounds at a dose of 12 umol/kg/day. *** represents p<0.001. (C) The levels of cytokine IFN-γ were detected in the sera under the same conditions as those described in (B).

FIG. 19. Synthesis of ZA-7-Nap conjugate 43

FIG. 20. Synthesis of ZA-7-Ibu conjugate 44

FIG. 21. Synthesis of ZA-9-Nap conjugate 45

FIG. 22. Synthesis of ZA-G-Nap conjugate 46

FIG. 23. Synthesis of ZA-G-Ibu conjugate 47

FIG. 24. Synthesis of OS-1-CA conjugate 48

FIG. 25. Synthesis of TP-1-CA conjugate 49

FIG. 26. Synthesis of PZA(1Et)-7-CA-amide conjugate 50

FIG. 27. Synthesis of PE-1-CA conjugate 51

FIG. 28. Synthesis of PE-2-CA conjugate 52

DETAILED DESCRIPTION OF THE INVENTION

The present invention provides compounds of Formula (A). Also provided are methods of using compounds of Formula (A), to treat and/or prevent influenza infection. The present invention further provides methods of using the compounds of Formula (A) as therapeutics, e.g., in the treatment and/or prevention of influenza infection.

As generally described above, provided herein are compounds of Formulae (A). In certain embodiments, the present disclosure provides compounds of Formula (A):

Compounds of Formula (A) comprises a first moiety and a second moiety, wherein the first moiety is an anti-influenza agent and the second moiety is an anti-inflammatory agent; and the first and second moieties are conjugated via a linker. In certain embodiments, the anti-inflammatory agent is a non-steroidal anti-inflammatory agent. In certain embodiments, the non-steroidal anti-inflammatory agent is an acid anti-inflammatory agent. In certain embodiments, the anti-influenza agent is a neuraminidase inhibitor. In certain embodiments, the anti-influenza agent is zanamivir (ZA), phosphono-zanamivir (PZA), oseltamivir (OS), tamiphosphor (TP), or peramivir (PE), or a derivative thereof. In certain embodiments, the linker is covalently linked to one or more of the anti-influenza agent and one or more of the anti-inflammatory agent. In certain embodiments, the linker the linker comprises an optionally substituted C₁₋₂₀ hydrocarbon chain or optionally substituted cycloalkyl, wherein one or more carbon units are replaced with NR^(N), O or S, wherein R^(N) is hydrogen, optionally substituted alkyl, or a nitrogen protecting group. In certain embodiments, the linker is cleavable by an esterase. In certain embodiments, the linker is not cleavable by an esterase. In certain embodiments, the linker is cleavable in lysosome. In certain embodiments, the linker is not cleavable in lysosome.

In certain embodiments, a compound of Formula (A) is of Formula (I) or (II):

or a pharmaceutically acceptable salt thereof; wherein:

X₁ is —O—, —O(C═O)—, —NH—, —NHCO—, —O(C═O)NH—, —O(C═S)NH—, —NH(C═O)NH—, or —NH(C═S)NH—;

L is a linker selected from the group consisting of a bond, —(CH₂)_(n)—, —(CH₂)_(n)O—, —O(CH₂)_(n)—, —(CH₂)_(n)—C(O)—, —(CH₂)_(n)—NH—, —NH—(CH₂)_(n)—, —C(O)(CH₂)_(n)—, —(CH₂)_(n)—NHC(O)—, —C(O)(CH₂)_(n)—NHC(O)—, (CH₂)_(n)SCH₂C(O)—, and —(CH₂CH₂O)_(m)—;

n is an integer from 1 to 8, inclusive;

m is an integer from 1 to 4, inclusive;

X₁ is covalently linked to L;

Y is a non-steroidal anti-inflammatory agent selected from the group consisting of aceclofenac, acemetacin, acetyl-sailcylic acid, 5-aminoacetylsalicylic acid, aldlofenac, amfenac, bendazac, benoxaprofen, bermoprofen, bromfenac, 5-bromosalicylic acid acetate, bucloxic acid, butibufen, caffeic acid, carprofen, chromoglycate, cinmetacin, clindanac, clopirac, sodium diclofenac, diflunisal, 3,4-dihydroxybenzoic acid, enfenamic acid, etodolac, felbinac, fenbufen, fenclozic acid, fendosal, fenoprofen, fentiazac, flufenac, flufenamic acid, flunixin, flunoxaprofen, flurbiprofen, 1-hydroxynaphthoic acid, ibufenac, ibuprofen, indomethacin, indoprofen, isofezolac, isoxepac, ketoprofen, ketorolac, loxoprofen, meclofenamic acid, mefenamic acid, mesalazine, 3,4-methylenedioxycinnamic acid, metiazinic acid, mofezolac, montelukast, mycophenolic acid, naproxen, niflumic acid, olsalazine, oxaceprol, oxaprozin, pyrazolac, pirprofen, pranoprofen, protizinic acid, sulindac, suprofen, suxibutazone, tiaprofenic acid, tinoridine acid, tolfenamic acid, tolmetin, tropesin, xenbucin, ximoprofen, zaltoprofen, and zomepirac;

Y is covalently linked to L via an acid group in the non-steroidal anti-inflammatory agent;

B is an azido, a hydroxyl, an unsubstituted or substituted amino, or an unsubstituted or substituted guanidino, or the corresponding salts with an anionic counterion selected from the group consisting of chloride, bromide, iodide, acetate, trifluoroacetate, phosphate, diphosphate, nitrate, sulfate, benzenesulfonate, benzoate, salicylate, hydroxynaphthoate, fumarate, maleate, lactate, malate, succinate, tartrate, citrate, glutamate, and gluconate; each instance of R¹ and R⁵ is, independently, hydrogen or optionally substituted C₁₋₆ alkyl; each instance of R² and R³ is, independently, hydrogen, optionally substituted alkyl, or optionally substituted acyl group; and

R⁴ is —COCH₃, —COCF₃, —SO₂CH₃ or —SO₂CF₃.

In certain embodiments, a compound of Formula (A) is of Formula (V) or (VI):

or a pharmaceutically acceptable salt thereof; wherein:

X₁ is —O—, —O(C═O)—, —NH—, —NHCO—, —O(C═O)NH—, —O(C═S)NH—, —NH(C═O)NH—, or —NH(C═S)NH—;

L is a linker selected from the group consisting of a bond, —(CH₂)_(n)—, —(CH₂)_(n)O—, —O(CH₂)_(n)—, —(CH₂)_(n)—C(O)—, —(CH₂)_(n)—NH—, —NH—(CH₂)_(n)—, —C(O)(CH₂)_(n)—, —(CH₂)_(n)—NHC(O)—, —C(O)(CH₂)_(n)—NHC(O)—, (CH₂)_(n)SCH₂C(O)—, and —(CH₂CH₂O)_(m)—;

n is an integer from 1 to 8;

m is an integer from 1 to 4;

X₁ is covalently linked to L;

Y is a non-steroidal anti-inflammatory agent selected from the group consisting of aceclofenac, acemetacin, acetyl-sailcylic acid, 5-aminoacetylsalicylic acid, aldlofenac, amfenac, bendazac, benoxaprofen, bermoprofen, bromfenac, 5-bromosalicylic acid acetate, bucloxic acid, butibufen, caffeic acid, carprofen, chromoglycate, cinmetacin, clindanac, clopirac, sodium diclofenac, diflunisal, 3,4-dihydroxybenzoic acid, enfenamic acid, etodolac, felbinac, fenbufen, fenclozic acid, fendosal, fenoprofen, fentiazac, flufenac, flufenamic acid, flunixin, flunoxaprofen, flurbiprofen, 1-hydroxynaphthoic acid, ibufenac, ibuprofen, indomethacin, indoprofen, isofezolac, isoxepac, ketoprofen, ketorolac, loxoprofen, meclofenamic acid, mefenamic acid, mesalazine, 3,4-methylenedioxycinnamic acid, metiazinic acid, mofezolac, montelukast, mycophenolic acid, naproxen, niflumic acid, olsalazine, oxaceprol, oxaprozin, pyrazolac, pirprofen, pranoprofen, protizinic acid, sulindac, suprofen, suxibutazone, tiaprofenic acid, tinoridine acid, tolfenamic acid, tolmetin, tropesin, xenbucin, ximoprofen, zaltoprofen, and zomepirac;

Y is covalently linked to L via an acid group in the non-steroidal anti-inflammatory agent;

B is an azido, a hydroxyl, an unsubstituted or substituted amino, or an unsubstituted or substituted guanidino, or the corresponding salts with an anionic counterion selected from the group consisting of chloride, bromide, iodide, acetate, trifluoroacetate, phosphate, diphosphate, nitrate, sulfate, benzenesulfonate, benzoate, salicylate, hydroxynaphthoate, fumarate, maleate, lactate, malate, succinate, tartrate, citrate, glutamate, and gluconate;

each instance of R¹, R⁵ and R⁶ is, independently, hydrogen or optionally substituted C₁₋₆ alkyl;

each instance of R² and R³ is, independently, hydrogen, optionally substituted alkyl, or optionally substituted acyl group; and

R⁴ is —COCH₃, —COCF₃, —SO₂CH₃ or —SO₂CF₃.

As generally defined herein, X₁ is —O—, —O(C═O)—, —NH—, —NHCO—, —O(C═O)NH—, —O(C═S)NH—, —NH(C═O)NH—, or —NH(C═S)NH—. In certain embodiments, X₁ is —O—. In certain embodiments, X₁ is —O(C═O)—. In certain embodiments, X₁ is —NH—. In certain embodiments, X₁ is —O(C═O)NH—. In certain embodiments, X₁ is —O(C═S)NH—. In certain embodiments, X₁ is —NH(C═O)NH—. In certain embodiments, X₁ is —NH(C═S)NH—.

As generally defined herein, L is a linker selected from the group consisting of —(CH₂)_(n)—, —(CH₂)_(n)O—, —O(CH₂)_(n)—, —(CH₂)_(n)—C(O)—, —(CH₂)_(n)—NH—, —NH—(CH₂)_(n)—, —C(O)(CH₂)_(n)—, —(CH₂)_(n)—NHC(O)—, —C(O)(CH₂)_(n)—NHC(O)—, (CH₂)_(n)SCH₂C(O)—, and —(CH₂CH₂O)_(m)—. As used herein, n is an integer from 1 to 8, inclusive. In certain embodiments, n is 1. In certain embodiments, n is 2. In certain embodiments, n is 3. In certain embodiments, n is 4. In certain embodiments, n is 5. In certain embodiments, n is 6. In certain embodiments, n is 7. In certain embodiments, n is 8. As used herein, m is an integer from 1 to 4, inclusive. In certain embodiments, m is 1. In certain embodiments, m is 2. In certain embodiments, m is 3. In certain embodiments, m is 4. In certain embodiments, L is —(CH₂)_(n)—. In certain embodiments, L is —(CH₂)₂—. In certain embodiments, L is —(CH₂)₃—. In certain embodiments, L is —(CH₂)₄—. In certain embodiments, L is —(CH₂)₅—. In certain embodiments, L is —(CH₂)₆—. In certain embodiments, L is —(CH₂)₇—. In certain embodiments, L is —(CH₂)₈—. In certain embodiments, m is 4. In certain embodiments, L is —(CH₂)_(n)O—. In certain embodiments, L is —(CH₂)₂O—. In certain embodiments, L is —(CH₂)₃O—. In certain embodiments, L is —(CH₂)₄O—. In certain embodiments, L is —(CH₂)₅O—. In certain embodiments, L is —(CH₂)₆O—. In certain embodiments, L is —(CH₂)₇O—. In certain embodiments, L is —(CH₂)₈O—. In certain embodiments, L is —(CH₂)_(n)NH—. In certain embodiments, L is —(CH₂)₂NH—. In certain embodiments, L is —(CH₂)₃NH—. In certain embodiments, L is —(CH₂)₄NH—. In certain embodiments, L is —(CH₂)₅NH—. In certain embodiments, L is —(CH₂)₆NH—. In certain embodiments, L is —(CH₂)₇NH—. In certain embodiments, L is —(CH₂)₈NH—.

In certain embodiments, —X₁-L- is —O(C═O)NH—(CH₂)_(n)O—. In certain embodiments, —X₁-L- is —O(C═O)NH—(CH₂)_(n)NH—. In certain embodiments, —X₁-L- is —(C═O)O—(CH₂)_(n)NH—. In certain embodiments, —X₁-L- is —(C═O)O—(CH₂)_(n)O—. In certain embodiments, —X₁-L- is —O—(CH₂)_(n)O—. In certain embodiments, —X₁-L- is —NH—(CH₂)_(n)O—.

As generally defined herein, Y is a non-steroidal anti-inflammatory agent selected from the group consisting of aceclofenac, acemetacin, acetyl-sailcylic acid, 5-aminoacetylsalicylic acid, aldlofenac, amfenac, bendazac, benoxaprofen, bermoprofen, bromfenac, 5-bromosalicylic acid acetate, bucloxic acid, butibufen, caffeic acid, carprofen, chromoglycate, cinmetacin, clindanac, clopirac, sodium diclofenac, diflunisal, 3,4-dihydroxybenzoic acid, enfenamic acid, etodolac, felbinac, fenbufen, fenclozic acid, fendosal, fenoprofen, fentiazac, flufenac, flufenamic acid, flunixin, flunoxaprofen, flurbiprofen, 1-hydroxynaphthoic acid, ibufenac, ibuprofen, indomethacin, indoprofen, isofezolac, isoxepac, ketoprofen, ketorolac, loxoprofen, meclofenamic acid, mefenamic acid, mesalazine, 3,4-methylenedioxycinnamic acid, metiazinic acid, mofezolac, montelukast, mycophenolic acid, naproxen, niflumic acid, olsalazine, oxaceprol, oxaprozin, pyrazolac, pirprofen, pranoprofen, protizinic acid, sulindac, suprofen, suxibutazone, tiaprofenic acid, tinoridine acid, tolfenamic acid, tolmetin, tropesin, xenbucin, ximoprofen, zaltoprofen, and zomepirac. Y is covalently linked to L via an acid group in the non-steroidal anti-inflammatory agent. In certain embodiments, Y is caffeic acid (CA). In certain embodiments, Y is mesalazine (ME). In certain embodiments, Y is naproxen (Nap). In certain embodiments, Y is ibuprofen (Ibu).

As generally defined herein, B is azido, hydroxyl, unsubstituted or substituted amino, or an unsubstituted or substituted guanidino, or the corresponding salts with an anionic counterion selected from the group consisting of chloride, bromide, iodide, acetate, trifluoroacetate, phosphate, diphosphate, nitrate, sulfate, benzenesulfonate, benzoate, salicylate, hydroxynaphthoate, fumarate, maleate, lactate, malate, succinate, tartrate, citrate, glutamate, and gluconate. In certain embodiments, B is azido. In certain embodiments, B is hydroxyl. In certain embodiments, B is substituted amino. In certain embodiments, B is unsubstituted amino. In certain embodiments, B is unsubstituted guanidino. In certain embodiments, B is substituted guanidino.

As generally defined herein, each instance of R¹ is independently, hydrogen or optionally substituted C₁₋₆ alkyl. In certain embodiments, R¹ is hydrogen. In certain embodiments, R¹ is substituted C₁₋₆ alkyl. In certain embodiments, R¹ is unsubstituted C₁₋₆ alkyl. In certain embodiments, R¹ is methyl. In certain embodiments, R¹ is ethyl. In certain embodiments, R¹ is propyl, butyl, pentyl, or hexyl. In certain embodiments, R¹ is isopropyl, isobutyl, or isoamyl. In certain embodiments, R¹ is isobutyl. In certain embodiments, R¹ is tert-butyl.

As generally defined herein, each instance of R⁵ is independently, hydrogen or optionally substituted C₁₋₆ alkyl. In certain embodiments, R⁵ is hydrogen. In certain embodiments, R⁵ is substituted C₁₋₆ alkyl. In certain embodiments, R⁵ is unsubstituted C₁₋₆ alkyl. In certain embodiments, R⁵ is methyl. In certain embodiments, R⁵ is ethyl. In certain embodiments, R⁵ is propyl, butyl, pentyl, or hexyl. In certain embodiments, R⁵ is isopropyl, isobutyl, or isoamyl. In certain embodiments, R⁵ is isobutyl. In certain embodiments, R⁵ is tert-butyl.

As generally defined herein, each instance of R⁶ is independently, hydrogen or optionally substituted C₁₋₆ alkyl. In certain embodiments, R⁶ is hydrogen. In certain embodiments, R⁶ is substituted C₁₋₆ alkyl. In certain embodiments, R⁶ is unsubstituted C₁₋₆ alkyl. In certain embodiments, R⁶ is methyl. In certain embodiments, R⁶ is ethyl. In certain embodiments, R⁶ is propyl, butyl, pentyl, or hexyl. In certain embodiments, R⁶ is isopropyl, isobutyl, or isoamyl. In certain embodiments, R⁶ is isobutyl. In certain embodiments, R⁶ is tert-butyl.

As generally defined herein, each instance of R² is, independently, hydrogen, optionally substituted alkyl, or optionally substituted acyl group. In certain embodiments, R² is hydrogen. In certain embodiments, R² is substituted C₁₋₆ alkyl. In certain embodiments, R² is unsubstituted C₁₋₆ alkyl. In certain embodiments, R² is methyl, ethyl, propyl, butyl, pentyl, or hexyl. In certain embodiments, R² is isopropyl, isobutyl, or isoamyl. In certain embodiments, R² is isobutyl. In certain embodiments, R² is tert-butyl. In certain embodiments, R² is acyl. In certain embodiments, R² is acetyl.

As generally defined herein, each instance of R³ is, independently, hydrogen, optionally substituted alkyl, or optionally substituted acyl group. In certain embodiments, R³ is hydrogen. In certain embodiments, R³ is substituted C₁₋₆ alkyl. In certain embodiments, R³ is unsubstituted C₁₋₆ alkyl. In certain embodiments, R³ is methyl, ethyl, propyl, butyl, pentyl, or hexyl. In certain embodiments, R³ is isopropyl, isobutyl, or isoamyl. In certain embodiments, R³ is isobutyl. In certain embodiments, R³ is tert-butyl. In certain embodiments, R³ is acyl. In certain embodiments, R³ is acetyl.

As generally defined herein, R⁴ is —COCH₃, —COCF₃, —SO₂CH₃ or —SO₂CF₃. In certain embodiments, R⁴ is —COCH₃. In certain embodiments, R⁴ is —COCF₃. In certain embodiments, R⁴ is —SO₂CH₃. In certain embodiments, R⁴ is —SO₂CF₃.

In certain embodiments, a compound of Formula (A) is of Formula (VII) or (VIII):

or a pharmaceutically acceptable salt thereof, wherein Y, B, R¹, R², R³, R⁴, R⁵, and R⁶ are as defined herein.

In another aspect, a provided compound is of formula (III) or (IV):

or a pharmaceutically acceptable salt thereof, wherein:

L, Y, B, R², R³, R⁴, and R⁶ are as defined above; and

X₂ is —O— or —NH—.

In certain embodiments, a provided compound is of formula (IX) or (X):

or a pharmaceutically acceptable salt thereof, wherein:

L, Y, B, and R⁴ are as defined above; and

X₂ is —O— or —NH—.

In another aspect, a provided compound is of formula (XI) or (XII):

or a pharmaceutically acceptable salt thereof, wherein:

L, Y, B, X₁, R¹, and R⁴ are as defined above;

X₂ is —O— or —NH—; and

R⁷ is hydrogen or optionally substituted C₁₋₆ alkyl.

As generally defined herein, X₂ is —O— or —NH—. In certain embodiments, X₂ is —O—. In certain embodiments, X₂ is —NH—. In certain embodiments, —X₂-L- is —O—(CH₂)_(n)O—. In certain embodiments, —X₂-L- is —O—(CH₂)_(n)NH—. In certain embodiments, —X₂-L- is —NH—(CH₂)_(n)O—. In certain embodiments, —X₂-L- is —NH—(CH₂)_(n)NH—.

As generally defined herein, R⁷ is independently, hydrogen or optionally substituted C₁₋₆ alkyl. In certain embodiments, R⁷ is hydrogen. In certain embodiments, R⁷ is substituted C₁₋₆ alkyl. In certain embodiments, R⁷ is unsubstituted C₁₋₆ alkyl. In certain embodiments, R⁷ is methyl. In certain embodiments, R⁷ is ethyl. In certain embodiments, R⁷ is propyl, butyl, pentyl, or hexyl. In certain embodiments, R⁷ is isopropyl, isobutyl, or isoamyl. In certain embodiments, R⁷ is isobutyl. In certain embodiments, R⁷ is tert-butyl.

The present invention further provides pharmaceutical compositions comprising a compound of Formula (A), or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, as described herein, and optionally a pharmaceutically acceptable excipient. In certain embodiments, the pharmaceutical composition of the invention comprises a compound of Formula (A), or a pharmaceutically acceptable salt thereof, and optionally a pharmaceutically acceptable excipient. In certain embodiments, the compound of Formula (A), or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, or prodrug thereof, is provided in an effective amount in the pharmaceutical composition. In certain embodiments, the effective amount is a therapeutically effective amount. In certain embodiments, the effective amount is a prophylactically effective amount.

Pharmaceutical compositions described herein can be prepared by any method known in the art of pharmacology. In general, such preparatory methods include the steps of bringing the compound of Formula (A) into association with a carrier and/or one or more other accessory ingredients, and then, if necessary and/or desirable, shaping and/or packaging the product into a desired single- or multi-dose unit.

Pharmaceutical compositions can be prepared, packaged, and/or sold in bulk, as a single unit dose, and/or as a plurality of single unit doses. As used herein, a “unit dose” is a discrete amount of the pharmaceutical composition comprising a predetermined amount of the active ingredient. The amount of the active ingredient is generally equal to the dosage of the active ingredient which would be administered to a subject and/or a convenient fraction of such a dosage such as, for example, one-half or one-third of such a dosage.

Relative amounts of the active ingredient, the pharmaceutically acceptable excipient, and/or any additional ingredients in a pharmaceutical composition of the invention will vary, depending upon the identity, size, and/or condition of the subject treated and further depending upon the route by which the composition is to be administered. By way of example, the composition may comprise between 0.1% and 100% (w/w) active ingredient.

Pharmaceutically acceptable excipients used in the manufacture of provided pharmaceutical compositions include inert diluents, dispersing and/or granulating agents, surface active agents and/or emulsifiers, disintegrating agents, binding agents, preservatives, buffering agents, lubricating agents, and/or oils. Excipients such as cocoa butter and suppository waxes, coloring agents, coating agents, sweetening, flavoring, and perfuming agents may also be present in the composition.

Exemplary diluents include calcium carbonate, sodium carbonate, calcium phosphate, dicalcium phosphate, calcium sulfate, calcium hydrogen phosphate, sodium phosphate lactose, sucrose, cellulose, microcrystalline cellulose, kaolin, mannitol, sorbitol, inositol, sodium chloride, dry starch, cornstarch, powdered sugar, and mixtures thereof.

Exemplary granulating and/or dispersing agents include potato starch, corn starch, tapioca starch, sodium starch glycolate, clays, alginic acid, guar gum, citrus pulp, agar, bentonite, cellulose, and wood products, natural sponge, cation-exchange resins, calcium carbonate, silicates, sodium carbonate, cross-linked poly(vinyl-pyrrolidone) (crospovidone), sodium carboxymethyl starch (sodium starch glycolate), carboxymethyl cellulose, cross-linked sodium carboxymethyl cellulose (croscarmellose), methylcellulose, pregelatinized starch (starch 1500), microcrystalline starch, water insoluble starch, calcium carboxymethyl cellulose, magnesium aluminum silicate (Veegum), sodium lauryl sulfate, quaternary ammonium compounds, and mixtures thereof.

Exemplary surface active agents and/or emulsifiers include natural emulsifiers (e.g. acacia, agar, alginic acid, sodium alginate, tragacanth, chondrux, cholesterol, xanthan, pectin, gelatin, egg yolk, casein, wool fat, cholesterol, wax, and lecithin), colloidal clays (e.g. bentonite (aluminum silicate) and Veegum (magnesium aluminum silicate)), long chain amino acid derivatives, high molecular weight alcohols (e.g. stearyl alcohol, cetyl alcohol, oleyl alcohol, triacetin monostearate, ethylene glycol distearate, glyceryl monostearate, and propylene glycol monostearate, polyvinyl alcohol), carbomers (e.g. carboxy polymethylene, polyacrylic acid, acrylic acid polymer, and carboxyvinyl polymer), carrageenan, cellulosic derivatives (e.g. carboxymethylcellulose sodium, powdered cellulose, hydroxymethyl cellulose, hydroxypropyl cellulose, hydroxypropyl methylcellulose, methylcellulose), sorbitan fatty acid esters (e.g. polyoxyethylene sorbitan monolaurate (Tween 20), polyoxyethylene sorbitan (Tween 60), polyoxyethylene sorbitan monooleate (Tween 80), sorbitan monopalmitate (Span 40), sorbitan monostearate (Span 60), sorbitan tristearate (Span 65), glyceryl monooleate, sorbitan monooleate (Span 80)), polyoxyethylene esters (e.g. polyoxyethylene monostearate (Myrj 45), polyoxyethylene hydrogenated castor oil, polyethoxylated castor oil, polyoxymethylene stearate, and Solutol), sucrose fatty acid esters, polyethylene glycol fatty acid esters (e.g. Cremophor™), polyoxyethylene ethers, (e.g. polyoxyethylene lauryl ether (Brij 30)), poly(vinyl-pyrrolidone), diethylene glycol monolaurate, triethanolamine oleate, sodium oleate, potassium oleate, ethyl oleate, oleic acid, ethyl laurate, sodium lauryl sulfate, Pluronic F-68, Poloxamer-188, cetrimonium bromide, cetylpyridinium chloride, benzalkonium chloride, docusate sodium, and/or mixtures thereof.

Exemplary binding agents include starch (e.g. cornstarch and starch paste), gelatin, sugars (e.g. sucrose, glucose, dextrose, dextrin, molasses, lactose, lactitol, mannitol, etc.), natural and synthetic gums (e.g. acacia, sodium alginate, extract of Irish moss, panwar gum, ghatti gum, mucilage of isapol husks, carboxymethylcellulose, methylcellulose, ethylcellulose, hydroxyethylcellulose, hydroxypropyl cellulose, hydroxypropyl methylcellulose, microcrystalline cellulose, cellulose acetate, poly(vinyl-pyrrolidone), magnesium aluminum silicate (Veegum), and larch arabogalactan), alginates, polyethylene oxide, polyethylene glycol, inorganic calcium salts, silicic acid, polymethacrylates, waxes, water, alcohol, and/or mixtures thereof.

Exemplary preservatives include antioxidants, chelating agents, antimicrobial preservatives, antifungal preservatives, alcohol preservatives, acidic preservatives, and other preservatives. In certain embodiments, the preservative is an antioxidant. In other embodiments, the preservative is a chelating agent.

Exemplary antioxidants include alpha tocopherol, ascorbic acid, acorbyl palmitate, butylated hydroxyanisole, butylated hydroxytoluene, monothioglycerol, potassium metabisulfite, propionic acid, propyl gallate, sodium ascorbate, sodium bisulfite, sodium metabisulfite, and sodium sulfite.

Exemplary chelating agents include ethylenediaminetetraacetic acid (EDTA) and salts and hydrates thereof (e.g., sodium edetate, disodium edetate, trisodium edetate, calcium disodium edetate, dipotassium edetate, and the like), citric acid and salts and hydrates thereof (e.g., citric acid monohydrate), fumaric acid and salts and hydrates thereof, malic acid and salts and hydrates thereof, phosphoric acid and salts and hydrates thereof, and tartaric acid and salts and hydrates thereof. Exemplary antimicrobial preservatives include benzalkonium chloride, benzethonium chloride, benzyl alcohol, bronopol, cetrimide, cetylpyridinium chloride, chlorhexidine, chlorobutanol, chlorocresol, chloroxylenol, cresol, ethyl alcohol, glycerin, hexetidine, imidurea, phenol, phenoxyethanol, phenylethyl alcohol, phenylmercuric nitrate, propylene glycol, and thimerosal.

Exemplary antifungal preservatives include butyl paraben, methyl paraben, ethyl paraben, propyl paraben, benzoic acid, hydroxybenzoic acid, potassium benzoate, potassium sorbate, sodium benzoate, sodium propionate, and sorbic acid.

Exemplary alcohol preservatives include ethanol, polyethylene glycol, phenol, phenolic compounds, bisphenol, chlorobutanol, hydroxybenzoate, and phenylethyl alcohol.

Exemplary acidic preservatives include vitamin A, vitamin C, vitamin E, beta-carotene, citric acid, acetic acid, dehydroacetic acid, ascorbic acid, sorbic acid, and phytic acid.

Other preservatives include tocopherol, tocopherol acetate, deteroxime mesylate, cetrimide, butylated hydroxyanisol (BHA), butylated hydroxytoluened (BHT), ethylenediamine, sodium lauryl sulfate (SLS), sodium lauryl ether sulfate (SLES), sodium bisulfite, sodium metabisulfite, potassium sulfite, potassium metabisulfite, Glydant Plus, Phenonip, methylparaben, Germall 115, Germaben II, Neolone, Kathon, and Euxyl.

Exemplary buffering agents include citrate buffer solutions, acetate buffer solutions, phosphate buffer solutions, ammonium chloride, calcium carbonate, calcium chloride, calcium citrate, calcium glubionate, calcium gluceptate, calcium gluconate, D-gluconic acid, calcium glycerophosphate, calcium lactate, propanoic acid, calcium levulinate, pentanoic acid, dibasic calcium phosphate, phosphoric acid, tribasic calcium phosphate, calcium hydroxide phosphate, potassium acetate, potassium chloride, potassium gluconate, potassium mixtures, dibasic potassium phosphate, monobasic potassium phosphate, potassium phosphate mixtures, sodium acetate, sodium bicarbonate, sodium chloride, sodium citrate, sodium lactate, dibasic sodium phosphate, monobasic sodium phosphate, sodium phosphate mixtures, tromethamine, magnesium hydroxide, aluminum hydroxide, alginic acid, pyrogen-free water, isotonic saline, Ringer's solution, ethyl alcohol, and mixtures thereof.

Exemplary lubricating agents include magnesium stearate, calcium stearate, stearic acid, silica, talc, malt, glyceryl behanate, hydrogenated vegetable oils, polyethylene glycol, sodium benzoate, sodium acetate, sodium chloride, leucine, magnesium lauryl sulfate, sodium lauryl sulfate, and mixtures thereof.

Exemplary natural oils include almond, apricot kernel, avocado, babassu, bergamot, black current seed, borage, cade, camomile, canola, caraway, carnauba, castor, cinnamon, cocoa butter, coconut, cod liver, coffee, corn, cotton seed, emu, eucalyptus, evening primrose, fish, flaxseed, geraniol, gourd, grape seed, hazel nut, hyssop, isopropyl myristate, jojoba, kukui nut, lavandin, lavender, lemon, litsea cubeba, macademia nut, mallow, mango seed, meadowfoam seed, mink, nutmeg, olive, orange, orange roughy, palm, palm kernel, peach kernel, peanut, poppy seed, pumpkin seed, rapeseed, rice bran, rosemary, safflower, sandalwood, sasquana, savoury, sea buckthorn, sesame, shea butter, silicone, soybean, sunflower, tea tree, thistle, tsubaki, vetiver, walnut, and wheat germ oils. Exemplary synthetic oils include, but are not limited to, butyl stearate, caprylic triglyceride, capric triglyceride, cyclomethicone, diethyl sebacate, dimethicone 360, isopropyl myristate, mineral oil, octyldodecanol, oleyl alcohol, silicone oil, and mixtures thereof.

Liquid dosage forms for oral and parenteral administration include pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups and elixirs. In addition to the active ingredients, the liquid dosage forms may comprise inert diluents commonly used in the art such as, for example, water or other solvents, solubilizing agents and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, dimethylformamide, oils (e.g., cottonseed, groundnut, corn, germ, olive, castor, and sesame oils), glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof. Besides inert diluents, the oral compositions can include adjuvants such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, and perfuming agents. In certain embodiments for parenteral administration, the conjugates of the invention are mixed with solubilizing agents such as Cremophor™, alcohols, oils, modified oils, glycols, polysorbates, cyclodextrins, polymers, and mixtures thereof.

Injectable preparations, for example, sterile injectable aqueous or oleaginous suspensions can be formulated according to the known art using suitable dispersing or wetting agents and suspending agents. The sterile injectable preparation can be a sterile injectable solution, suspension, or emulsion in a nontoxic parenterally acceptable diluent or solvent, for example, as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that can be employed are water, Ringer's solution, U.S.P. and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose any bland fixed oil can be employed including synthetic mono- or diglycerides. In addition, fatty acids such as oleic acid are used in the preparation of injectables.

The injectable formulations can be sterilized, for example, by filtration through a bacterial-retaining filter, or by incorporating sterilizing agents in the form of sterile solid compositions which can be dissolved or dispersed in sterile water or other sterile injectable medium prior to use.

In order to prolong the effect of a drug, it is often desirable to slow the absorption of the drug from subcutaneous or intramuscular injection. This can be accomplished by the use of a liquid suspension of crystalline or amorphous material with poor water solubility. The rate of absorption of the drug then depends upon its rate of dissolution which, in turn, may depend upon crystal size and crystalline form. Alternatively, delayed absorption of a parenterally administered drug form is accomplished by dissolving or suspending the drug in an oil vehicle.

Compositions for rectal or vaginal administration are typically suppositories which can be prepared by mixing the conjugates of this invention with suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the active ingredient.

Solid dosage forms for oral administration include capsules, tablets, pills, powders, and granules. In such solid dosage forms, the active ingredient is mixed with at least one inert, pharmaceutically acceptable excipient or carrier such as sodium citrate or dicalcium phosphate and/or (a) fillers or extenders such as starches, lactose, sucrose, glucose, mannitol, and silicic acid, (b) binders such as, for example, carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidinone, sucrose, and acacia, (c) humectants such as glycerol, (d) disintegrating agents such as agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate, (e) solution retarding agents such as paraffin, (f) absorption accelerators such as quaternary ammonium compounds, (g) wetting agents such as, for example, cetyl alcohol and glycerol monostearate, (h) absorbents such as kaolin and bentonite clay, and (i) lubricants such as talc, calcium stearate, magnesium stearate, solid polyethylene glycols, sodium lauryl sulfate, and mixtures thereof. In the case of capsules, tablets, and pills, the dosage form may include a buffering agent.

Solid compositions of a similar type can be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like. The solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings and other coatings well known in the art of pharmacology. They may optionally comprise opacifying agents and can be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions which can be used include polymeric substances and waxes. Solid compositions of a similar type can be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polethylene glycols and the like.

The active ingredient can be in a micro-encapsulated form with one or more excipients as noted above. The solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings, release controlling coatings and other coatings well known in the pharmaceutical formulating art. In such solid dosage forms the active ingredient can be admixed with at least one inert diluent such as sucrose, lactose or starch. Such dosage forms may comprise, as is normal practice, additional substances other than inert diluents, e.g., tableting lubricants and other tableting aids such a magnesium stearate and microcrystalline cellulose. In the case of capsules, tablets, and pills, the dosage forms may comprise buffering agents. They may optionally comprise opacifying agents and can be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions which can be used include polymeric substances and waxes.

Dosage forms for topical and/or transdermal administration of a compound of this invention may include ointments, pastes, creams, lotions, gels, powders, solutions, sprays, inhalants and/or patches. Generally, the active ingredient is admixed under sterile conditions with a pharmaceutically acceptable carrier and/or any needed preservatives and/or buffers as can be required. Additionally, the present invention contemplates the use of transdermal patches, which often have the added advantage of providing controlled delivery of an active ingredient to the body. Such dosage forms can be prepared, for example, by dissolving and/or dispensing the active ingredient in the proper medium. Alternatively or additionally, the rate can be controlled by either providing a rate controlling membrane and/or by dispersing the active ingredient in a polymer matrix and/or gel.

Suitable devices for use in delivering intradermal pharmaceutical compositions described herein include short needle devices such as those described in U.S. Pat. Nos. 4,886,499; 5,190,521; 5,328,483; 5,527,288; 4,270,537; 5,015,235; 5,141,496; and 5,417,662. Intradermal compositions can be administered by devices which limit the effective penetration length of a needle into the skin, such as those described in PCT publication WO 99/34850 and functional equivalents thereof. Jet injection devices which deliver liquid vaccines to the dermis via a liquid jet injector and/or via a needle which pierces the stratum corneum and produces a jet which reaches the dermis are suitable. Jet injection devices are described, for example, in U.S. Pat. Nos. 5,480,381; 5,599,302; 5,334,144; 5,993,412; 5,649,912; 5,569,189; 5,704,911; 5,383,851; 5,893,397; 5,466,220; 5,339,163; 5,312,335; 5,503,627; 5,064,413; 5,520,639; 4,596,556; 4,790,824; 4,941,880; 4,940,460; and PCT publications WO 97/37705 and WO 97/13537. Ballistic powder/particle delivery devices which use compressed gas to accelerate the compound in powder form through the outer layers of the skin to the dermis are suitable. Alternatively or additionally, conventional syringes can be used in the classical mantoux method of intradermal administration.

Formulations suitable for topical administration include, but are not limited to, liquid and/or semi liquid preparations such as liniments, lotions, oil-in-water and/or water-in-oil emulsions such as creams, ointments, and/or pastes, and/or solutions and/or suspensions. Topically-administrable formulations may, for example, comprise from about 1% to about 10% (w/w) active ingredient, although the concentration of the active ingredient can be as high as the solubility limit of the active ingredient in the solvent. Formulations for topical administration may further comprise one or more of the additional ingredients described herein.

A pharmaceutical composition of the invention can be prepared, packaged, and/or sold in a formulation suitable for pulmonary administration via the buccal cavity. Such a formulation may comprise dry particles which comprise the active ingredient and which have a diameter in the range from about 0.5 to about 7 nanometers or from about 1 to about 6 nanometers. Such compositions are conveniently in the form of dry powders for administration using a device comprising a dry powder reservoir to which a stream of propellant can be directed to disperse the powder and/or using a self-propelling solvent/powder dispensing container such as a device comprising the active ingredient dissolved and/or suspended in a low-boiling propellant in a sealed container. Such powders comprise particles wherein at least 98% of the particles by weight have a diameter greater than 0.5 nanometers and at least 95% of the particles by number have a diameter less than 7 nanometers. Alternatively, at least 95% of the particles by weight have a diameter greater than 1 nanometer and at least 90% of the particles by number have a diameter less than 6 nanometers. Dry powder compositions may include a solid fine powder diluent such as sugar and are conveniently provided in a unit dose form.

Low boiling propellants generally include liquid propellants having a boiling point of below 65° F. at atmospheric pressure. Generally the propellant may constitute 50 to 99.9% (w/w) of the composition, and the active ingredient may constitute 0.1 to 20% (w/w) of the composition. The propellant may further comprise additional ingredients such as a liquid non-ionic and/or solid anionic surfactant and/or a solid diluent (which may have a particle size of the same order as particles comprising the active ingredient).

Pharmaceutical compositions of the invention formulated for pulmonary delivery may provide the active ingredient in the form of droplets of a solution and/or suspension. Such formulations can be prepared, packaged, and/or sold as aqueous and/or dilute alcoholic solutions and/or suspensions, optionally sterile, comprising the active ingredient, and may conveniently be administered using any nebulization and/or atomization device. Such formulations may further comprise one or more additional ingredients including, but not limited to, a flavoring agent such as saccharin sodium, a volatile oil, a buffering agent, a surface active agent, and/or a preservative such as methylhydroxybenzoate. The droplets provided by this route of administration may have an average diameter in the range from about 0.1 to about 200 nanometers.

Formulations described herein as being useful for pulmonary delivery are useful for intranasal delivery of a pharmaceutical composition of the invention. Another formulation suitable for intranasal administration is a coarse powder comprising the active ingredient and having an average particle from about 0.2 to 500 micrometers. Such a formulation is administered by rapid inhalation through the nasal passage from a container of the powder held close to the nares.

Formulations for nasal administration may, for example, comprise from about as little as 0.1% (w/w) to as much as 100% (w/w) of the active ingredient, and may comprise one or more of the additional ingredients described herein. A pharmaceutical composition of the invention can be prepared, packaged, and/or sold in a formulation for buccal administration. Such formulations may, for example, be in the form of tablets, and/or lozenges made using conventional methods, and may contain, for example, 0.1 to 20% (w/w) active ingredient, the balance comprising an orally dissolvable and/or degradable composition and, optionally, one or more of the additional ingredients described herein. Alternately, formulations for buccal administration may comprise a powder and/or an aerosolized and/or atomized solution and/or suspension comprising the active ingredient. Such powdered, aerosolized, and/or aerosolized formulations, when dispersed, may have an average particle and/or droplet size in the range from about 0.1 to about 200 nanometers, and may further comprise one or more of the additional ingredients described herein.

A pharmaceutical composition of the invention can be prepared, packaged, and/or sold in a formulation for ophthalmic administration. Such formulations may, for example, be in the form of eye drops including, for example, a 0.1/1.0% (w/w) solution and/or suspension of the active ingredient in an aqueous or oily liquid carrier. Such drops may further comprise buffering agents, salts, and/or one or more other of the additional ingredients described herein. Other opthalmically-administrable formulations which are useful include those which comprise the active ingredient in microcrystalline form and/or in a liposomal preparation. Ear drops and/or eye drops are contemplated as being within the scope of this invention.

Although the descriptions of pharmaceutical compositions provided herein are principally directed to pharmaceutical compositions which are suitable for administration to humans, it will be understood by the skilled artisan that such compositions are generally suitable for administration to animals of all sorts. Modification of pharmaceutical compositions suitable for administration to humans in order to render the compositions suitable for administration to various animals is well understood, and the ordinarily skilled veterinary pharmacologist can design and/or perform such modification with ordinary experimentation.

Compounds provided herein are typically formulated in dosage unit form for ease of administration and uniformity of dosage. It will be understood, however, that the total daily usage of the compositions of the present invention will be decided by the attending physician within the scope of sound medical judgment. The specific therapeutically effective dose level for any particular subject or organism will depend upon a variety of factors including the disease being treated and the severity of the disorder; the activity of the specific active ingredient employed; the specific composition employed; the age, body weight, general health, sex and diet of the subject; the time of administration, route of administration, and rate of excretion of the specific active ingredient employed; the duration of the treatment; drugs used in combination or coincidental with the specific active ingredient employed; and like factors well known in the medical arts.

The compounds and compositions provided herein can be administered by any route, including enteral (e.g., oral), parenteral, intravenous, intramuscular, intra-arterial, intramedullary, intrathecal, subcutaneous, intraventricular, transdermal, interdermal, rectal, intravaginal, intraperitoneal, topical (as by powders, ointments, creams, and/or drops), mucosal, nasal, bucal, sublingual; by intratracheal instillation, bronchial instillation, and/or inhalation; and/or as an oral spray, nasal spray, and/or aerosol. Specifically contemplated routes are oral administration, intravenous administration (e.g., systemic intravenous injection), regional administration via blood and/or lymph supply, and/or direct administration to an affected site. In general the most appropriate route of administration will depend upon a variety of factors including the nature of the agent (e.g., its stability in the environment of the gastrointestinal tract), and/or the condition of the subject (e.g., whether the subject is able to tolerate oral administration).

The exact amount of a compound required to achieve an effective amount will vary from subject to subject, depending, for example, on species, age, and general condition of a subject, severity of the side effects or disorder, identity of the particular compound(s), mode of administration, and the like. The desired dosage can be delivered three times a day, two times a day, once a day, every other day, every third day, every week, every two weeks, every three weeks, or every four weeks. In certain embodiments, the desired dosage can be delivered using multiple administrations (e.g., two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, thirteen, fourteen, or more administrations).

In certain embodiments, an effective amount of a compound for administration one or more times a day to a 70 kg adult human may comprise about 0.0001 mg to about 3000 mg, about 0.0001 mg to about 2000 mg, about 0.0001 mg to about 1000 mg, about 0.001 mg to about 1000 mg, about 0.01 mg to about 1000 mg, about 0.1 mg to about 1000 mg, about 1 mg to about 1000 mg, about 1 mg to about 100 mg, about 10 mg to about 1000 mg, or about 100 mg to about 1000 mg, of a compound per unit dosage form.

In certain embodiments, the compounds of Formula (I) may be at dosage levels sufficient to deliver from about 0.001 mg/kg to about 100 mg/kg, from about 0.01 mg/kg to about 50 mg/kg, preferably from about 0.1 mg/kg to about 40 mg/kg, preferably from about 0.5 mg/kg to about 30 mg/kg, from about 0.01 mg/kg to about 10 mg/kg, from about 0.1 mg/kg to about 10 mg/kg, and more preferably from about 1 mg/kg to about 25 mg/kg, of subject body weight per day, one or more times a day, to obtain the desired therapeutic effect.

It will be appreciated that dose ranges as described herein provide guidance for the administration of provided pharmaceutical compositions to an adult. The amount to be administered to, for example, a child or an adolescent can be determined by a medical practitioner or person skilled in the art and can be lower or the same as that administered to an adult.

It will be also appreciated that a compound or composition, as described herein, can be administered in combination with one or more additional pharmaceutical agents. The compounds or compositions can be administered in combination with additional pharmaceutical agents that improve their bioavailability, reduce and/or modify their metabolism, inhibit their excretion, and/or modify their distribution within the body. It will also be appreciated that the therapy employed may achieve a desired effect for the same disorder, and/or it may achieve different effects.

The compound or composition can be administered concurrently with, prior to, or subsequent to, one or more additional pharmaceutical agents, which may be useful as, e.g., combination therapies. Pharmaceutical agents include therapeutically active agents. Pharmaceutical agents also include prophylactically active agents. Each additional pharmaceutical agent may be administered at a dose and/or on a time schedule determined for that pharmaceutical agent. The additional pharmaceutical agents may also be administered together with each other and/or with the compound or composition described herein in a single dose or administered separately in different doses. The particular combination to employ in a regimen will take into account compatibility of the inventive compound with the additional pharmaceutical agents and/or the desired therapeutic and/or prophylactic effect to be achieved. In general, it is expected that the additional pharmaceutical agents utilized in combination be utilized at levels that do not exceed the levels at which they are utilized individually. In some embodiments, the levels utilized in combination will be lower than those utilized individually.

Exemplary additional pharmaceutical agents include, but are not limited to, anti-proliferative agents, anti-cancer agents, anti-diabetic agents, anti-inflammatory agents, immunosuppressant agents, and a pain-relieving agent. Pharmaceutical agents include small organic molecules such as drug compounds (e.g., compounds approved by the U.S. Food and Drug Administration as provided in the Code of Federal Regulations (CFR)), peptides, proteins, carbohydrates, monosaccharides, oligosaccharides, polysaccharides, nucleoproteins, mucoproteins, lipoproteins, synthetic polypeptides or proteins, small molecules linked to proteins, glycoproteins, steroids, nucleic acids, DNAs, RNAs, nucleotides, nucleosides, oligonucleotides, antisense oligonucleotides, lipids, hormones, vitamins, and cells.

Also encompassed by the invention are kits (e.g., pharmaceutical packs). The inventive kits may be useful for preventing and/or treating a influenza infection. The kits provided may comprise an inventive pharmaceutical composition or compound and a container (e.g., a vial, ampule, bottle, syringe, and/or dispenser package, or other suitable container). In some embodiments, provided kits may optionally further include a second container comprising a pharmaceutical excipient for dilution or suspension of an inventive pharmaceutical composition or compound. In some embodiments, the inventive pharmaceutical composition or compound provided in the container and the second container are combined to form one unit dosage form.

Thus, in one aspect, provided are kits including a first container comprising a compound described herein, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, and prodrug thereof, or a pharmaceutical composition thereof. In certain embodiments, the kit of the invention includes a first container comprising a compound described herein, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition thereof. In certain embodiments, the kits are useful in preventing and/or treating influenza infection in a subject. In certain embodiments, the kits further include instructions for administering the compound, or a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, stereoisomer, isotopically labeled derivative, and prodrug thereof, or a pharmaceutical composition thereof, to a subject to prevent and/or treat an influenza infection.

The terms used in this specification generally have their ordinary meanings in the art, within the context of the invention, and in the specific context where each term is used. Certain terms that are used to describe the invention are discussed below, or elsewhere in the specification, to provide additional guidance to the practitioner regarding the description of the invention. For convenience, certain terms may be highlighted, for example using italics and/or quotation marks. The use of highlighting has no influence on the scope and meaning of a term; the scope and meaning of a term is the same, in the same context, whether or not it is highlighted. It will be appreciated that same thing can be said in more than one way. Consequently, alternative language and synonyms may be used for any one or more of the terms discussed herein, nor is any special significance to be placed upon whether or not a term is elaborated or discussed herein. Synonyms for certain terms are provided. A recital of one or more synonyms does not exclude the use of other synonyms. The use of examples anywhere in this specification including examples of any terms discussed herein is illustrative only, and in no way limits the scope and meaning of the invention or of any exemplified term. Likewise, the invention is not limited to various embodiments given in this specification.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. In the case of conflict, the present document, including definitions will control.

As used herein, the term therapeutic drug is intended to define a subclass of drugs which have therapeutic (and not just prophylactic) properties. Such drugs may have prophylactic activity in addition to therapeutic potential. They find application in the treatment of existing diseases, infections or conditions.

As used herein, the term co-administration, as used in the context of the administration of the various components of the compositions, vaccines etc. of the invention, is intended to cover the sequential, concurrent or separate administration of the referenced components. Concurrent administration therefore covers the case where the referenced components are physically mixed prior to administration. Sequential administration covers circumstances in which the referenced components are administered separately with some degree of temporal separation (typically from several minutes to several hours, although in some embodiments the administration of the co-administered components may be separated by a period of one or more days).

“Synergism” may be measured by combination index (CI). The combination index method was described by Chou and Talalay. (Chou, T.-C. The median-effect principle and the combination index for quantitation of synergism and antagonism, p. 61-102. In T.-C. Chou and D. C. Rideout (ed.), Synergism and antagonism in chemotherapy. Academic Press, San Diego, Calif. (1991); Chou, T.-C., and P. Talalay. Quantitative analysis of dose-effect relationships: the combined effects of multiple drugs on enzyme inhibitors. Adv. Enzyme Regul. 22:27-55 (1984)). A CI value of 0.90 or less is considered synergistic, with values of 0.85 being moderately synergistic and values below 0.70 being significantly synergistic. CI values of 0.90 to 1.10 are considered to be nearly additive and higher values are antagonistic. Table 1. Synergism/antagonism as a function of CI values

TABLE 1 Synergism/antagonism as a function of CI values CI Value Interpretation >10 Very strong antagonism 3.3-10  Strong antagonism 1.45-3.3  Antagonism  1.2-1.45 Moderate antagonism 1.1-1.2 Slight antagonism 0.9-1.1 Additive 0.85-0.9  Slight synergism  0.7-0.85 Moderate synergism 0.3-0.7 Synergism 0.1-0.3 Strong synergism <0.1 Very strong synergism

It is noted that determination of synergy may be affected by biological variability, dosage, experimental conditions (temperature, pH, oxygen tension, etc.), treatment schedule and combination ratio.

Novel bifunctional, dual-targeted conjugates of zanamivir with immuno-modulating drugs that are expected to yield greater synergistic effect than the sum of single components. (Meunier, B. Acc. Chem. Res. 2008, 41, 69) FIG. 1 shows the putative working mechanism of such dual-targeted zanamivir conjugates. Therapy with designed multiple ligands (DMLs) may have advantage of better pharmacological effect and improved safety over combination of individual drugs in treatment of multi-genic and multi-target diseases such as cancer, diabetes and infectious diseases. (Morphy, R. et al. Drug Discov. Today 2004, 9, 641; Morphy, R. & Rankovic, Z. J. Med. Chem. 2006, 49, 4961; Zimmermann, G. R. et al. Drug Discov. Today 2007, 12, 34).

Dual-targeted anti-influenza drugs according to the invention disclosed herein are designed for virus inactivation and cytokine suppression (FIG. 1). Due to high affinity of ZA with NA, the ZA conjugates would be brought to the influenza infected tissues allowing the anti-inflammatory moiety of the conjugates to suppress the induction of proinflammatory cytokines in a highly effective manner. Three zanamivir conjugates ZA-7-CA (1), ZA-7-ME (3) and ZA-7-CA-amido (7) that have the linkage at the C-7 position of zanamivir to caffeic acid (CA) or mesalazine (ME) via ester or amide bonds (FIG. 2) are provided. For comparison, other zanamivir C-7 conjugates ZA-7-MCA (2), ZA-7-HNAP (4), ZA-7-DHBA (5), ZA-7-HP (6) and ZA-7-HNAP-amido (8) as well as zanamivir C-1 conjugates ZA-1-CA (9), ZA-1-MCA (10), ZA-1-ME (11) and ZA-1-HNAP (12) are provided. According to the structural analysis of the ZA-NA complex, (Varghese, J. N. et al. Protein Sci. 1995, 4, 1081) the 7-OH group of ZA exposes to water and makes no direct interaction with the active site of influenza NA protein.

It has been shown that the ZA derivatives with modification at the C7 hydroxyl group retain good NA inhibitory activity. (Andrews, D. M. et al. Eur. J. Med. Chem. 1999, 34, 563; Honda, T. et al. Bioorg. Med. Chem. Lett. 2002, 12, 1921; Honda, T. et al. Bioorg. Med. Chem. Lett. 2002, 12, 1925) Thus, the ZA-7-conjugates 1-8 with improved lipophilicity are expected to exhibit good anti-influenza activity. The ZA-1-conjugates 9-12 with ester linkage would undergo enzymatic hydrolysis in body to give the active ZA drug, so that 9-12 may act as long-acting neuraminidase inhibitors. (Liu, Z.-y. et al. Bioorg. Med. Chem. Lett. 2007, 17, 4851; Yamashita, M. et al. Antimicrob. Agents Chemother. 2009, 53, 186; Kubo, S. et al. Antimicrob. Agents Chemother. 2010, 54, 1256; Ishizuka, H. et al. J. Clin. Pharmacol. 2010, 50, 1319)

Caffeic acid (CA) and its ester derivatives are known to exhibit anti-inflammatory effect in addition to other biological activities. (Michaluart, P. et al. Cancer Res. 1999, 59, 2347; Jung, W. K. et al. Int. J. Biochem. Cell Biol. 2008, 40, 2572; Shin, K.-M. et al. Biochem. Pharmacol. 2004, 68, 2327) Caffeic esters can suppress NF-κB and its downstream mediators, iNOS and COX-2, by inhibiting the formation of NF-κB with DNA complex. (Natarajan, K. et al. Proc. Natl. Acad. Sci. USA 1996, 93, 9090; Chiang, Y. M. et al. Brit. J. Pharmacol. 2005, 146, 352) Mesalazine (ME) is an anti-inflammatory drug used to treat ulcerative colitis and Crohn's disease, which are two major human chronic inflammatory bowel diseases. (Kaiser, G. C. t al. Gastroenterology 1999, 116, 602; Kruis, W. et al. Gut 2001, 49, 783; Sandborn, W. J. et al. Aliment. Pharmacol. Ther. 2007, 26, 987) Mesalazine is also known as an agonist of peroxisome proliferator-activator receptor (PPAR-γ) for induction of anti-inflammatory effects. (Rousseaux, C. et al. J. Exp. Med. 2005, 201, 1205)

Chemical Synthesis of Anti-Influenza Conjugates

FIG. 3 shows the general synthetic strategy for zanamivir conjugates 1-12. Sialic acid was converted to ethyl ester derivative 13 by slight modification of the reported method for the corresponding methyl ester 13a. (Chandler, M. et al. J. Chem. Soc., Perkin Trans. 1 1995, 1173) Compound 13 was then transformed to 14, which has a free hydroxyl group readily for further linkage with appropriate anti-inflammation drugs to form zanamivir-7-conjugates, such as compounds 1-8. Alternatively, ester 14 was hydrolyzed to give the corresponding acid, which could undergo condensation reactions with appropriate alcohols to give zanamivir-1-conjugates, such as compounds 9-12. The synthetic procedures for these conjugates are shown in FIGS. 4-15, FIG. 19 and FIG. 20. Compound 14 also served as the common intermediate for the synthesis of zanamivir conjugates containing anti-inflammatory agents at the 9- or guanidine positions, such as those shown in FIGS. 21-23.

Compound 14 was treated with 4-nitrophenyl chloroformate and DMAP in dry pyridine to afford an active carbonate 15, (Ying, L. & Gervay-Hague, J. ChemBioChem 2005, 6, 1857; Wen, W.-H. et al. J. Med. Chem. 2009, 52, 4903) which was subsequently reacted with 3-amino-1-propanol to give a carbamate derivative 16 (FIG. 4). All the protecting groups in 16 were removed to afford the ZA-7-HP conjugate 6 bearing an N-(3-hydroxypropyl)carmamate moiety at the C-7 position of ZA.

The condensation reaction of 16 with the bis-allyl ether of caffeic acid (18) was carried out by using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDCI) and 4-dimethylaminopyridine (DMAP) as the promoters to yield the desired coupling product 19 with an ester linkage (FIG. 5). Compound 19 was treated with KOH (1 M aqueous solution) at room temperature for a selective saponification of the ethyl ester at C-1 without cleavage of the ester linkage between zanamivir and the caffeic moiety. Palladium-catalyzed deallylation was effected, and the subsequent treatment with trifluoroacetic acid (TFA) removed the acetonide and tert-butoxycarbonyl (Boc) protecting groups to afford the ZA-7-CA conjugate (1).

By similar procedures, the coupling reactions of 16 with 3,4-(methylenedioxy)cinnamic acid (MCA), mesalazine allyl ether (22), 1-hydroxy-2-naphthoic acid (HNAP) allyl ether (25) and 3,4-dihydroxybenzoic acid (DHBA) bis-allyl ether (28) gave, respectively, compounds 20, 23, 26 and 29, which were elaborated to the ZA-7-MCA (2), ZA-7-ME (3), ZA-7-HNAP (4) and ZA-7-DHBA (5) conjugates after removal of the protecting groups (FIGS. 6-9).

The CA derivative 32 bearing a terminal amino group was prepared, and then reacted with the activated carbonate 15 to give the ZA-7-CA-amide conjugate (7) after removal of the protecting groups (FIG. 10). The ZA-7-HNAP-amido conjugate (8) was similarly synthesized via the coupling reaction of carbonate 15 with the amine-annexed HNAP derivative 35, followed by removal of protecting groups (FIG. 11). The amide linkage in ZA-7-CA-amide and ZA-7-HNAP-amide conjugates is considered more stable than the enzymatically cleavable ester linkage in the conjugates 1-5.

To prepare ZA-1-conjugates, acid 37 was treated with KOH (1 equiv) to afford the carboxylate potassium salt, which reacted with 3-iodo-1-propanol to provide ester 38. The coupling reactions of 38 with CA allyl ether 18, MCA, ME allyl ether 22 and HNAP allyl ether 25 produced the ZA-1-CA (9), ZA-1-MCA (10), ZA-1-ME (11) and ZA-1-HNAP (12) conjugates, respectively, after removal of the protecting groups (FIGS. 12-15).

FIGS. 19 and 20 show the syntheses of ZA-7-Nap conjugate (43) and ZA-7-Ibu (44). Methyl ester 13a, a counterpart of the ethyl ester 13, was prepared according to the reported procedure (Chandler, M. et al. J. Chem. Soc., Perkin Trans. 11995, 1173). Saponification of 13a with NaOMe in methanol, followed by acid-catalyzed acetalization with 2,2-dimethoxypropane afforded compound 14a, which was treated with 4-nitrophenyl chloroformate and 4-dimethylaminopyridine in anhydrous pyridine to give carbonate 15a. The coupling reaction of 15a with naproxen 3-aminopropyl ester in the presence of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDCI) and 4-dimethylaminopyridine (DMAP) gave ZA-7-Nap conjugate 43 after removal of the acetal and t-Boc protecting groups. By a similar procedure, the coupling reaction of 15a with ibuprofen 3-aminopropyl ester was carried out, and the protecting groups were removed to afford ZA-7-Ibu conjugate 44.

To synthesize zanamivir conjugates at 9-position, the acetyl groups compound 13 was selectively hydrolyzed to give a triol compound 56 (FIG. 21). The coupling reaction of naproxen with 56 occurred regioselectively at the primary alcohol, giving compound 57. The ethyl ester in 57 was selectively saponified under mild alkaline condition, and ZA-9-Nap conjugate 45 was obtained after removal of the t-Boc groups.

FIGS. 22 and 23 show the syntheses of zanamivir conjugates at the guanidine position. For example, naproxen was condensed with tert-butyl[imino(methylthio)methyl]carbamate to give compound 60. The reaction of 60 with the amine obtained from hydrogenation of azide 59 in the presence of HgCl₂ and Et₃N was carried out to give compound 60. Removal the t-Boc group and the subsequent hydrolysis thus yield ZA-G-Nap 46. By a similar procedure using ibuprofen as the starting material, ZA-G-Ibu conjugate 47 was prepared (FIG. 23).

FIG. 24 shows the synthesis of oseltamivir-caffeate conjugate OS-1-CA (48). Oseltamivir N-Boc derivative 64 was treated with 3-iodo-1-propanol in alkaline conditions to afford ester 65, which underwent a condensation reaction with bis-allyl ether of caffeic acid (18) to give the coupling product 66. The desired OS-1-CA conjugate (48) was thus obtained by removal of the allyl and Boc protecting groups.

FIG. 25 shows the synthesis of tamiphosphor-caffeate conjugate TP-1-CA (49). The iodine compound 67 was prepared as previously described. (Shie, J.-J. et al. Angew. Chem. Int. Ed. 2008, 47, 5788) The reaction of 67 with 2-phosphora-1,3-dioxin-2-one (68) by the catalysis of palladium gave the phosphonate product 69. Saponification of 69 afforded the phosphonate monoester 70, which reacted with the activated caffeic acid O-Su ester (71) to give the coupling product 72. Removal of the allyl and Boc protecting groups in 72 gave the desired TP-1-CA conjugate 49.

FIG. 26 shows the synthesis of the conjugate 50 derived from phosphono-zanamivir monoethyl ester and caffeic acid. According to the reported procedure, (Horn, E. J. et al. Carbohydr. Res. 2008, 343, 936) sialic acid was treated with acetic anhydride in pyridine to afford the peracetylation product, which was heated at 100° C. to induce decarboxylation to furnish compound 73. Using trimethylsilyl diethyl phosphite as an appropriate nucleophile, the substitution reaction of 73 was carried out by the promotion of trimethylsilyl trifluoromethylsulfonate (TMSOTf) to give the phosphonate compound 74 as a mixture of a and β anomers (2:3). These anomers were separated by chromatography and characterized by NMR spectral analyses. For synthetic purpose, the anomeric mixture 74 without further separation was treated with N-bromosuccinimide (NBS) under photochemical conditions to give an α-bromination compound, which was subsequently treated with pyridine to give the conjugated phosphonate 75. Compound 75 was converted into the oxazoline 76 in the presence of acetic anhydride, acetic acid and concentrated H₂SO₄. The regio- and stereoselective ring-opening reaction of oxazoline 76 with azidotrimethylsilane was carried out to afford the azido compound 77. After catalytic hydrogenation, the amine intermediate was protected as the t-Boc derivative 78. Saponification of 78 gave a triol product, which reacted with 2,2′-dimethoxypropane to give acetal 79. The free hydroxyl group at the C-7 position was activated as the p-nitrophenyl carbonate 80, which reacted with the amine 81 derived from caffeic acid to afford carbamate 82. On refluxing with LiBr, the diethyl phosphonate was converted to monoethyl phosphonate. The subsequent treatment with TFA thus culminated the synthesis of PZA(1Et)-7-CA-amide conjugate 50.

FIG. 27 shows the synthesis of peramivir-caffeate conjugate PE-1-CA (51). According to the reported method, (Mineno, T.; Miller, M. J. J. Org. Chem. 2003, 68, 6591.) (1R,4S)-2-azabicyclo[2.2.1]hept-5-en-3-one 83 was treated with HCl in MeOH to give 4-aminocyclopent-2-ene carboxylate 84. The amino group was protected to give 85, which was subject to 1,3-diploar cycloaddition with the nitrile oxide generated from 2-ethylbutanal oxime, giving compound 86. After hydrogenolysis, the newly formed amino group was subject to acetylation. The t-Boc group was removed in acidic conditions, and then treated with 1,3-bis(tert-butoxycarbony)-2-methyl-2-thiopseudourea in the presence of HgCl₂ to give compound 90. After saponification, the resulting carboxylic acid underwent condensation reaction with the caffeic acid derivative 92 to give compound 93. Saponification of 93 in mild alkaline conditions, followed by removal of t-Boc group with TFA, gave PE-1-CA (51).

FIG. 28 shows the synthesis of peramivir-caffeate conjugate PE-2-CA (52). Compound 90 was activated with p-nitrophenyl chloroformate, giving a carbonate intermediate, and reacted with the caffeic acid derivative 95 to afford carbamate 96. The desired product PE-2-CA (52) was thus obtained after saponification of the methyl ester and removal of the allyl and t-Boc protecting groups.

Bioactivity of Conjugates Inhibition of Influenza Virus Neuraminidase A/WSN/1933 (H1N1)

The NA inhibition activities of zanamivir and its conjugates 1-12 were evaluated using a fluorogenic substrate 2′-(4-methyl-umbelliferyl)-α-D-N-acetylneuraminic acid (MUNANA) against influenza A/WSN/1933 (H1N1) viruses (Table 1). The ZA-7-conjugates 1-8 exibited NA inhibitory activities with IC₅₀ values in nanomolar range comparable to ZA. In contrast, the ZA-1-conjugates 9-12 lost NA inhibitory activity, supporting that the C-1 group of carboxylic acid played an essential role in electrostatic interactions with the three arginine (Arg118, Arg292 and Arg371) residues in the active site of NA. (Chong, A. K. J. et al. Eur. J. Biochem. 1992, 207, 335; von Itzstein, M. et al. Nature 1993, 363, 418; Taylor, N. R. & von Itzstein, M. J. Med. Chem. 1994, 37, 616; Lew, W. et al. Curr. Med. Chem. 2000, 7, 663; Russell, R. J. et al. Nature 2006, 443, 45) The anti-inflammation agents CA, MCA, and ME alone did not exhibit any NA inhibitory activity.

Anti-Influenza Activity

The cytopathic prevention assays in Madin-Darby canine kidney (MDCK) cells were conducted to evaluate the anti-influenza activity. To our anticipation, the ZA-7-conjugates were potent inhibitors against human H1N1 virus (Table 2). The ZA-1-conjugates 9-12 also resumed anti-influenza activity in the cell-based assays. In sharp contrast, our assays indicated that 3-hydroxypropyl caffeate, 3-(tert-butoxyamido)propyl caffeate and chlorogenic acid (a caffeic ester formed with L-quinic acid) were inactive to H1N1 influenza viruses. Thus, the observed potent anti-influenza activity of conjugates ZA-1-CA (1) and ZA-7-CA (9) may be related to the enzymatic cleavage of the ester linkages to release the active ZA drug. The anti-influenza activity could not be caused by caffeic esters alone. (Salomon, R. et al. Proc. Natl. Acad. Sci. U.S.A. 2007, 104, 12479)

The activity against avian H5N1 influenza virus was also evaluated by the cytopathic prevention assays using the recombinant NIBRG-14 (A/Viet Nam/1194/2004) virus. In particular, the ZA-7-CA conjugate showed very high activity against the avian H5N1 virus with an EC₅₀ value of 50 nM, about 13-fold greater potency than zanamivir (EC₅₀=680 nM). The ZA-7-ME and ZA-7-HP conjugates showed the inhibitory activities similar to zanamivir against H5N1 virus with EC₅₀ of 430 and 820 nM, similar to the potency of ZA.

TABLE 2 Influenza neuraminidase inhibition (IC₅₀) and anti-influenza activity (EC₅₀) against A/WSN/1933 (H1N1) virus Compound^(a) IC₅₀ (nM)^(b) EC₅₀ (nM)^(b) cLog P^(c) ZA 2.4-7.0 (8) 3.5-20.2 (8) −4.13 CA >10⁵ >10⁵ 1.42 MCA >10⁵ >10⁵ 2.53 ME >10⁵ >10⁵ 0.46 HNAP >10⁵ >10⁵ 3.29 ZA-7-CA (1) 2.9-7.4 (8) 1.4-10.6 (8) −1.63 ZA-7-MCA (2) 5.4-7.9 (3) 66, 146 −0.52 ZA-7-ME (3) 8.6-56.3 (2)  3.3, 7.9  −2.26 ZA-7-HNAP (4) 0.7-3.6 (3)   50-168 (3) 0.65 ZA-7-DHBA (5) 2.9 53 −1.13 ZA-7-HP (6) 11.7-19.8 (2)  105, 371  −3.54 ZA-7-CA-amide (7) 41.3-60.3 (3)  5.1, 5.9  −2.36 ZA-7-HNAP-amide (8) 3.1 6.6 −0.39 ZA-1-CA (9) 2853 25 −3.60 ZA-1-MCA (10) 3986 28 −2.49 ZA-1-ME (11) 996 16 −3.49 ZA-1-HNAP (12) 590 48 −0.59 ^(a)All the test compounds are nontoxic to MDCK cells at the highest testing concentrations (100 μM). ^(b)IC₅₀ and EC₅₀ values were determined by nonparametric curve fitting of assay results, and the shown data are ranges of assay results with number of assays in parentheses. ^(c)Calculated values of octanol-water partition coefficients using Advanced Chemistry Development (ACD/Labs) Software V12.01.

ZA-7-Nap (43) and ZA-7-Ibu (44) conjugates showed potent inhibitory activity against the neuraminidase of influenza A/WSN/1933 (H1N1) virus with IC₅₀ values of 3-6 nM, similar to zanamivir. ZA-7-Nap enhanced activity with EC₅₀ value of 0.8 nM in protection of the MDCK cells infected by A/WSN/1933 viruses, whereas ZA-7-Ibu showed the anti-influenza activity similar to zanamivir (EC₅₀=14 nM in this experiment).

ZA-G-Nap (46) and ZA-G-Ibu (47) conjugates showed decreased affinity to neuraminidase (IC₅₀=44-81 nM). However, they possess the anti-influenza activity (EC₅₀=18-20 nM) against A/WSN/1933 virus similar to zanamivir.

OS-1-CA (48) and TP-1-CA (49) conjugates showed mild inhibitory activity against the neuraminidase of influenza A/WSN/1933 (H1N1) virus with IC₅₀ values of 2.4 μM and 0.7 μM, respectively. In the cell-based assay, OS-1-CA resumed high activity with EC₅₀ value of 73 nM, about 6-fold greater potency than oseltamivir in protection of the MDCK cells infected by A/WSN/1933 viruses.

Animal Experiments

Zanamivir (Relenza™) is an inhaled drug requiring a dose of 20 mg/50 kg/day (1.2 μmol/kg/day) by intranasal administrations twice (with half of the dosage at each administration) for treatment of influenza infected patients. Accordingly, the test compounds at the indicated dosages (12, 1.2, 0.12, 0.012 or 0.0012 μmol/kg/day) were intranasally administered to mice twice-daily on days 1-4, and day 0 before infection with lethal doses (10 LD₅₀) of influenza A/Cal/07/2009 H1N1 (FIGS. 16A-D) or NIBRG-14 (A/VietNam/1194/2004) H5N1 (FIGS. 17A and 17B) viruses. The survival of mice was monitored for 14 days. The synthetic conjugates ZA-7-CA (1) and Za-7-ME (3) at a dose of 12 μmol/kg/day showed greater protection than ZA, CA or ME in H1N1 virus infected mice (FIG. 16A). The combination therapy with ZA+CA showed slight increase in survival time compared to those treated with ZA or CA alone at a dose of 1.2 μmol/kg/day, but all the mice died at day 12 except for those treated with conjugate 1 (FIG. 16B). The group of mice treated with conjugate 1 at a low dose of 0.12 μmol/kg/day all survived at day 14 (FIG. 16C). The survival rate of mice decreased to 40% at day 14 on treatment with even less dose (0.012 μmol/kg/day) of 1. However, 0.0012 μmol/kg/day of 1 did not show significant protection of mice from influenza virus infection (data not shown). The ZA-7-CA-amide conjugate (7) with amide linkage at a dose of 1.2 μmol/kg/day also showed 100% protection from virus-induced death, greater protection than ZA or the combination therapy of ZA+CA in treatment of the H1N1 virus infected mice (both showed 10% survival at day 14) (FIG. 16D). Treatment with 0.12 μmol/kg/day of conjugate 7 led to 50% survival at day 14, but treatment with lower dose at 0.012 μmol/kg/day did not improve the survival rate.

The mice infected by NIBRG-14 H5N1 virus all survived by treatment with intranasal administration of conjugate 1 at 12 μmol/kg/day, whereas the combination therapy ZA+CA or ZA+ME at the same dose did not improve the survival rate, nor did the treatment with ZA alone (FIG. 17A). Conjugate 3 did not provide better protection effect than ZA against avian H5N1 virus (FIG. 17A), though this conjugate showed good activity in treatment of human H1N1 influenza (FIG. 16A). Conjugate 7 at 12 μmol/kg/day showed greater potency against H5N1 virus, in comparison with ZA alone or the combination therapy of ZA+CA (FIG. 17B). On treatment with conjugate 7 at a dose of 1.2 μmol/kg/day, improved survival rate was still noted (FIG. 17B).

Cytokine Suppression

The potential effects of synthetic conjugates on inhibition of the production of pro-inflammatory cytokines were determined in lipopolysaccharide (LPS)-activated RAW264.7 cells (FIG. 18A). Apigenin, a flavone known to possess anti-inflammatory activity, was used as positive control (FIG. 18A, lanes 3 and 4). The anti-inflammatory drugs (CA, MCA and ME in lanes 5, 6, and 11-14) and their ZA conjugates 1 and 3 (lanes 7-10) did cause dose-dependent decreases in the mRNAs encoding IL-6 and TNF-α.

For the H5N1 virus infected mice, the test compounds at 12 μmol/kg/day were intranasally administered, and the serum samples were collected at 72 h to determine the levels of IL-6 and INF-γ. The results indicated that the cytokine levels in the serum were lower in the groups treated respectively with ZA-7-CA and ZA-7-ME conjugates (FIGS. 18B and 18C) by comparison with the groups treated with the combination of ZA+CA and ZA+ME or zanamivir alone. These preliminary results suggested that the ZA-7-CA and ZA-7-ME conjugates acted to prevent the accumulation of pro-inflammatory cytokines at the transcriptional level.

Biologic tests clearly indicated that the survival rates in the mice challenged with H1N1 or H5N1 viruses were tremendously improved by treatment with the zanamivir conjugates, such as ZA-7-CA (1), ZA-7-ME (3) and ZA-7-CA-amide (7), in comparison with the combination treatment with zanamivir and anti-inflammatory drugs. Though the detailed processes for such synergistic effect on influenza treatment is not fully resolved, some observations from our tests may give insights into the working mechanism. These dual-targeted conjugates not only inactivated influenza viruses but also decreased proinflammatory cytokines as shown by the cell-based assays and mice challenge experiments. It was suggested that the zanamivir component in the conjugates might act as a specific binder to influenza virus, and brought the anti-inflammatory component to the location where the virus-induced cytokine storm might be effectively suppressed.

The ester bonds could be hydrolyzed by endogenous esterases in the blood, liver and kidney. (Lavis, L. D. ACS Chem. Biol. 2008, 3, 203.] As shown in Table 1, the zanamivir-1-conjugates did not exhibit good NA inhibition; however, they resumed anti-influenza activity (EC₅₀=16-48 nM) in the cell-based assays likely due to enzymatic cleavage of the C-1 ester to release the active zanamivir drug. Indeed, incubation of ZA-1-HNAP conjugate (12, m/z 561, [M+H]⁺) in rat plasma at 37° C. for 24 h gave the expected degradative products of ZA (m/z 333, [M+H]⁺) and HNAP (m/z 189, [M+H]⁺) as evidence by the MALDI-TOFMS and LC-QTOFMS spectrometric analyses. Under the similar incubation conditions, the ester linkages in ZA-1-CA conjugate (9, m/z 561 [M+H]⁺) were also cleaved by plasma esterases to release the two active components ZA and CA (m/z 181, [M+H]⁺). The enzymatic cleavage of ZA-7-CA conjugate (1, m/z 596 [M+H]⁺) in plasma also released a degradation product ZA-7-HP (6) showing the protonation signal at m/z 434. However, the ZA-7-CA-amide conjugate (7, m/z 617 [M+H]⁺) with amide linkage was relatively stable in plasma. Since both ZA-7-CA and ZA-7-CA-amide conjugates exhibited good anti-influenza activity, enzymatic cleavage of the ester linkage in zanamivir-7-conjugates seemed not to be a prerequisite for the synergistic dual-targeted functions in NA inhibition and cytokine suppression.

In another aspect, zanamivir has poor bioavailability (estimated to be 5% in humans) due to its low lipophilicity and rapid metabolism. (Cass, L. M. R. et al. Clin. Pharmacokinet. 1999, 36, 1) Lipophilicity is a key determinant of the pharmacokinetic behavior of drugs. The partition coefficient (P) between octanol and water is usually taken as a suitable measure of lipophilicity. The calculated value clog P for zanamivir is −4.13 (Table 1). The ZA-7-conjugates with CA, MCA, ME and HNAP that contain aromatic moieties would have higher lipophilicity than zanamivir, as shown by the clog P values of these conjugates in the range of −2.36 to 0.65 (Table 2). The zanamivir C-1 ester containing a lipophilic alkoxyalkyl moiety has been shown to exhibit significant protective effect in the mice infected by influenza virus. (Liu, Z.-y. et al. Bioorg. Med. Chem. Lett. 2007, 17, 4851) In this study, the ZA-7-conjugates with increased lipophilicity may also improve pharmacokinetic properties for better anti-influenza activity.

To treat influenza infection that is accompanied by the virus-induced cytokine storm, the drug comprising an anti-influenza moiety linked with an anti-inflammatory agent may provide a better therapy. Our study indicated that the dual-targeted ZA conjugates exhibited synergistic anti-influenza activity in comparison with ZA alone or the combination of ZA and anti-inflammatory agent.

EXAMPLES

Without intent to limit the scope of the invention, exemplary instruments, apparatus, methods and their related results according to the embodiments of the present invention are given below. Note that titles or subtitles may be used in the examples for convenience of a reader, which in no way should limit the scope of the invention. Moreover, certain theories are proposed and disclosed herein; however, in no way they, whether they are right or wrong, should limit the scope of the invention so long as the invention is practiced according to the invention without regard for any particular theory or scheme of action.

Example 1 Instrumentation

Melting points were recorded on a Yanaco or Electrothermal MEL-TEMP 1101D apparatus in open capillaries and are not corrected. Optical rotations were measured on digital polarimeter of Japan JASCO Co. DIP-1000. [α]_(D) values are given in units of 10⁻¹ deg cm² g⁻¹. Infrared (IR) spectra were recorded on Nicolet Magna 550-II or Thermo Nicolet 380 FT-IR spectrometers. UV-visible spectra were measured on a Perkin Elmer Lambda 35 spectrophotometer. Nuclear magnetic resonance (NMR) spectra were obtained on Bruker Advance-400 (400 MHz) spectrometer. Chemical shifts (δ) are given in parts per million (ppm) relative to δ_(H) 7.24/δ_(C) 77.0 (central line of t) for CHCl₃/CDCl₃, δ_(H) 4.80 for H₂O/D₂O, δ_(H) 3.31/δ_(C) 48.2 for CD₃OD, or δ_(H) 2.49/δ_(C) 39.5 for DMSO-d₆. The splitting patterns are reported as s (singlet), d (doublet), t (triplet), q (quartet), m (multiplet), dd (double of doublets) and br (broad). Coupling constants (J) are given in Hz. Distortionless enhancement polarization transfer (DEPT) spectra were taken to determine the types of carbon signals. The ESI-MS experiments were conducted on a Bruker Daltonics BioTOF III high-resolution mass spectrometer. The MALDI-MS measurements were performed on a Bruker Daltonics Ultraflez II MALDI-TOF/TOF 2000 mass spectrometer. The 2,5-dihydroxybenzoic acid (DHB), as MALDI matrix, was photoionized at different irradiances of a UV laser with λ_(max) at 337 nm and 355 nm.

Example 2 Chemical Materials

All the reagents and solvents were reagent grade and were used without further purification unless otherwise specified. All solvents were anhydrous grade unless indicated otherwise. CH₂Cl₂ was distilled from CaH₂. All non-aqueous reactions were carried out in oven-dried glassware under a slight positive pressure of argon unless otherwise noted. Reactions were magnetically stirred and monitored by thin-layer chromatography on silica gel using aqueous p-anisaldehyde as visualizing agent. Silica gel (0.040-0.063 mm particle sizes) and LiChroprep® RP-18 (0.040-0.063 mm particle sizes) were used for column chromatography. Flash chromatography was performed on silica gel of 60-200 m particle size. Molecular sieves were activated under high vacuum at 220° C. over 6 hours. Purity of compounds I-12 was assessed to be 2 95% by HPLC (Agilent HP-1100) with detection at 254 or 360 nm wavelength.

Example 3 Biological Materials

Influenza virus A/Vietnam/1194/2004 RG14 (H5N1) and A/California/7/2009 (H1N1) were from the Center for Disease Control and Prevention in Taiwan and influenza A/WSN/1933 (H1N1) was from Dr. Shin-Ru Shih in Chang Gung University in Taiwan. All Viruses were cultured in the allantoic cavities of 10-day-old embryonated chicken eggs for 72 h, and purified by sucrose gradient centrifugation. Madin-Darby canine kidney (MDCK) cells were obtained from American Type Culture Collection (Manassas, Va.), and were grown in DMEM (Dulbecco's modified Eagle medium, GibcoBRL) containing 10% fetal bovine serum (GibcoBRL) and penicillin-streptomycin (GibcoBRL) at 37° C. under 5% CO₂.

Example 4 Determination of Influenza Virus TCID₅₀

The TCID₅₀ (50% tissue culture infectious dose) was determined by serial dilution of the influenza virus stock onto 100 μL MDCK cells at 1×10⁵ cells/mL in 96-well microplates. The infected cells were incubated at 37° C. under 5.0% CO₂ for 48 h and added to each wells with 100 μL per well of CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay reagent (Promega). After incubation at 37° C. for 15 min, absorbance at 490 nm was read on a plate reader. Influenza virus TCID₅₀ was determined using Reed-Muench method. (Reed, L. J.; Muench, H. Am. J. Epidemiol. 1938, 27, 493; Burleson, F. G. et al. Virology, a Laboratory Manual, 1992)

Example 5 Determination of NA Activity by Fluorescent Assay

The neuraminidase (NA) activity was measured using diluted allantoic fluid harvested from influenza A/WSN/1933 (H1N1) infected embryonated eggs. A fluorometric assay was used to determine the NA activity with the fluorogenic substrate 2′-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid (MUNANA; Sigma). The fluorescence of the released 4-methylumbelliferone was measured in Envision plate reader (Perkin-Elmer, Wellesley, Mass.) using excitation and emission wavelengths of 365 and 460 nm, respectively. Neuraminidase activity was determined at 200 μM of MUNANA. Enzyme activity was expressed as the fluorescence increase during 15 min incubation at room temperature.

Example 6 Determination of IC₅₀ of NA Inhibitor

NA inhibition was determined by mixing inhibitor and neuraminidase for 10 min at room temperature followed by the addition of 200 μM of substrate. Inhibitor IC₅₀ value were determined from the dose-response curves by plotting the percent inhibition of NA activity versus inhibitor concentrations using Graph Pad Prism 4.

Example 7 Determination of EC₅₀ of NA inhibitor

The anti-flu activities of neuraminidase inhibitors were measured by the EC₅₀ values that were the concentrations of NA inhibitor for 50% protection of the H1N1 CPE activities. Fifty μL diluted H1N1 at 100 TCID₅₀ were mixed with equal volumes of NA inhibitors at varied concentrations. The mixtures were used to infect 100 μL of MDCK cells at 1×10⁵ cells/mL in 96-wells. After 48 h incubation at 37° C. under 5.0% CO₂, the cytopathic effects (CPE) were determined with CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay reagent as described above. Inhibitor EC₅₀ value were determined by fitting the curve of percent CPE versus the concentrations of NA inhibitor using Graph Pad Prism 4.

Example 8 Animal Experiments

Female BALB/c mice (4-5 weeks old) were anesthetized with zoletil and inoculated intranasally with 25 μL of infectious influenza virus. Synthetic compounds and zanamivir were administered to groups of 10 mice at dosages of 0.0012, 0.012, 0.12, 1.2 and 12 μmol per kg of body weight per day by intranasal instillation twice daily for 5 days. Control (placebo) mice received PBS on the same schedule. Four hours after the first dose of drug, mice were inoculated with 10 MLD₅₀ of influenza virus [A/Cal/07/2009 (H1N1) or NIBRG-14 (A/Vietnam/1194/2004 (H5N1)]. Mice were observed daily for 14 days for survival.

Example 9 RNA Isolation and Real-Time Polymerase Chain Reaction (RT-PCR)

Raw264.7 cells were cultured in 6-well plates at 3×10⁶ cells per well for 24 h and treated with test compounds for 30 min followed by addition of 100 ng mL LPS for another 6 h. The cells were collected and stored at −80° C. until use. Total RNA was extracted using Trizol® (Invitrogen) and processed for RT-PCR as described previously. (Hsieh, Y. S. Y. et al. Bioorg. Med. Chem. Lett. 2008, 16, 6054-6068)

Example 10 Cytokine Determination by ELISA

The concentrations of IL-6 and IFN-γ in the mice serum were assayed using the Quantikine® mouse ELISA kits (R&D Systems, Minneapolis, Minn.) according to the manufacturer's protocol.

Example 11 Incubation of Zanamivir Conjugates in Plasma and Mass Spectrometric Analyses

Sprague Dawley® rat plasma (550 μL) and 50 μL of analyte were mixed to yield a final concentration of 1 mM. The mixture was incubated at 37° C. for 24 h, extracted with methanol (3.6 mL) by vortex mixing at 4° C. for 2H, and then subjected to centrifugation at 10,000 rpm for 20 min. The precipitate was removed by filtration. The filtrate was concentrated under reduced pressure, and the content of enzymatic cleavage products was analyzed by MALDI-TOFMS.

Alternatively, the mixture resulted from incubation was subjected to LC-QTOF MS analysis. The sample was loaded onto a Sep-Pak® Plus tC18 cartridge (Waters Corp), and flushed with deionized water (2×2 mL) to remove excess salts and proteins, followed by elution with MeOH/H₂O (1:1, 4×2 mL) to collect analyte. The analyte was evaporated to dryness, and re-dissolved in CH₃CN/H₂O (1:9, 100 μL) for LC-QTOF MS analysis on a nano Acquity UPLC BEH C18 column (1.7 μm, 75 μm×250 mm, Waters Corp). The injection volume was 2-5 μL and the total run time was 50 min. A mixture of 0.1% formic acid in water (mobile phase A) and 0.1% formic acid in acetonitrile (mobile phase B) was used with a gradient program at a flow rate of 0.3 μL/min at ambient temperature. To analyze the product mixture formed by incubation of ZA-1-HNAP in plasma, the gradient program of mobile phase was set: a linear increase from 10:90 to 85:15 B/A in 25 min, and kept at 85:15 B/A for 10 min. The column was washed for 15 min before returning to original conditions.

Example 12 Determination of Octanol-Buffer Partition Coefficients

Solutions of each compound (100 μg/mL) were prepared in octanol saturated phosphate buffered saline (0.01 M, pH 7.4). These aqueous solutions were then equilibrated at 37° C. with an equivalent volume (0.5 mL) of buffer saturated octanol using magnetic stirring at 1200 rpm for 24 hours. Five replicates of each determination were carried out to assess reproducibility. The octanol and aqueous phases were then separated by centrifugation at 3000 rpm for 15 min. Each sample (300 μL) of aqueous layer was diluted with PBS to 3 mL, and organic layer was diluted with MeOH to 3 mL. The concentration of drug was measured UV spectrophotometry at λ_(max) (MeOH) 290 nm (ε=14440) and λ_(max) (PBS) 290 nm (ε=4610) at pH 7.4 for the chromophore of 1-hydroxy-2-naphthoic acid. From these data, the apparent octanol/buffer (pH 7.4) partition coefficient, DB=[Bt]oct/[Bt]aq, is determined, where [Bt]oct and [Bt]aq are the concentrations of the drug in organic and aqueous phases, respectively.

Example 13 Synthesis of Zanamivir (FIG. 3)

Zanamivir (ZA) was prepared from sialic acid by a procedure similar to the previous report (Chandler, M. et al. J. Chem. Soc., Perkin Trans. 11995, 1173) through the ethyl ester 13 instead of the corresponding methyl ester.

Example 14 Synthesis of ZA-7-HP Conjugate 6 (FIG. 4) 5-Acetylamino-4-[2,3-bis(tert-butoxycarbonyl)guanidino]-6-[(2,2-dimethyl-[1,3]dioxolan-4-yl)-hydroxy-methyl]-5,6-dihydro-4H-pyran-2-carboxylic acid ethyl ester (14)

To a solution of 13 (1100 mg, 1.60 mmol) in anhydrous ethanol (10 mL) was added NaOEt (54.4 mg, 0.80 mmol). The mixture was stirred at room temperature for 1 h, neutralized by Dowex 50W×8 (H⁺), filtered, and concentrated under reduced pressure to afford yellow solids. The residue was dissolved in anhydrous acetone (10 mL), and p-toluene sulfonic acid and 2,2-dimethoxypropane were added. The mixture was stirred at room temperature for 12 h. After concentration, the residue was purified by silica gel column chromatography (EtOAc/hexane=2:3) to afford compound 14 (635 mg, 66%). C₂₇H₄₄N₄O₁₁; white solid, mp 118-120° C.; TLC (EtOAc/hexane=2:3) R_(f)=0.22; [α]²⁰ _(D) −33.4 (c=1.0, CHCl₃); IR ν_(max) (neat) 3309, 2981, 1725, 1647, 1609, 1559, 1369, 1251, 1151 cm⁻¹; ¹H NMR (400 MHz, CDCl₃) δ 11.30 (1H, s), 8.54 (1H, d, J=7.2 Hz), 7.91 (1H, d, J=4.4 Hz), 5.71 (1H, d, J=2.4 Hz), 5.21 (1H, br s), 5.08-5.05 (1H, m), 4.33-4.30 (1H, m), 4.19-4.13 (2H, m), 4.11-4.00 (2H, m), 3.98-3.88 (1H, m), 3.45 (1H, d, J=3.6 Hz), 1.93 (3H, s), 1.44 (9H, s), 1.41 (9H, s), 1.34 (3H, s), 1.28-1.22 (6H, m); ¹³C NMR (100 MHz, CDCl₃) δ 173.8, 162.2, 161.3, 157.5, 152.5, 147.0, 108.9, 106.3, 84.2, 79.9, 78.5, 74.1, 69.6, 67.3, 61.4, 51.9, 48.3, 28.1 (3×), 27.9 (3×), 26.9, 25.1, 22.8, 13.9; ESI-HRMS (negative mode) calcd for C₂₇H₄₃N₄O₁₁: 599.2928, found: m/z 599.2926 [M−H]⁻.

5-Acetylamino-4-[2,3-bis(tert-butoxycarbonyl)guanidino]-6-[(2,2-dimethyl-[1,3]dioxolan-4-yl)-(4-nitrophenoxy)carbonyloxy)methyl]-5,6-dihydro-4H-pyran-2-carboxylic acid ethyl ester (15)

A solution of compound 14 (540 mg, 0.8 mmol), 4-nitrophenyl chloroformate (1131 mg, 5.6 mmol) and 4-dimethylaminopyridine (327 mg, 5.6 mmol) in anhydrous pyridine (12 mL) was stirred at room temperature for 16 h. After concentration, the mixture was added EtOAc, and then filtered. The filtrate was concentrated under reduced pressure, and purified by silica gel column chromatography (EtOAc/hexane=1:2) to give carbonate 15 (360 mg, 60%). C₃₄H₄₇N₅O₁₅; white solid, mp 127-128° C.; TLC (EtOAc/hexane=2:3) R_(f)=0.35; [α]²⁰ _(D) −45.7 (c=1.0, CHCl₃); IR ν_(max) (neat) 2981, 1777, 1729, 1645, 1612, 1527, 1369, 1253, 1221, 1149 cm⁻¹; ¹H NMR (400 MHz, CDCl₃) δ 11.29 (1H, s), 8.44 (1H, d, J=8.8 Hz), 8.16 (2H, d, J=9.2 Hz), 7.44 (2H, d, J=9.2 Hz), 6.54 (1H, d, J=8.8 Hz), 5.81 (1H, d, J=2.0 Hz), 5.25 (1H, dd, J=5.6, 1.6 Hz), 5.13-5.08 (1H, m), 4.38-4.31 (2H, m), 4.19-4.14 (4H, m), 4.09-3.99 (1H, m), 1.82 (3H, s). 1.39 (18H, s), 1.31 (3H, s), 1.28-1.22 (6H, m); ¹³C NMR (100 MHz, CDCl₃) δ 171.2, 162.5, 160.9, 157.0, 155.7, 152.4, 152.3, 145.3, 125.0 (2×), 122.2 (2×), 108.9, 108.5, 83.8, 79.6, 77.3, 75.2, 73.9, 65.3, 61.5, 60.2, 48.5, 48.0, 28.0 (3×), 27.8 (3×), 26.1, 25.3, 22.8, 13.9; ESI-HRMS calcd for C₃₄H₄₈N₅O₁₅: 766.3147, found: m/z 766.3146 [M+H]⁺.

5-Acetylamino-6-[(2,2-dimethyl-[1,3]dioxolan-4-yl)-3-[(hydroxypropyl)carbamoyloxy]methyl-4-[2,3-bis(tert-butoxycarbonyl)guanidino]-5,6-dihydro-4H-pyran-2-carboxylic acid ethyl ester (16)

To a solution of carbonate 15 (400 mg, 0.52 mmol) in CH₃CN (7 mL) was added 3-amino-1-propanol (0.084 mL, 1.1 mmol) and Et₃N (0.153 mL, 1.1 mmol). The mixture was stirred at room temperature for 2H, and then concentrated under reduced pressure. The residue was partitioned between EtOAc and 1 M HCl. The organic layer was washed with saturated NaHCO₃ and brine, dried over MgSO₄, filtered, and concentrated by rotary evaporation under reduced pressure. The crude material was purified by silica gel column chromatography (EtOAc/hexane=3:1) to yield carbamate 16 (320 mg, 88%). C₃₁H₅₁N₅O₁₃; white solid, mp 135-137° C.; TLC (EtOAc/hexane=4:1) R_(f)=0.38; [α]²⁰ _(D) −24.2 (c=1.0, CHCl₃); IR ν_(max) (neat) 3315, 2981, 1731, 1643, 1613, 1250, 1144, 1057 cm⁻¹; ¹H NMR (400 MHz, CDCl₃) δ 11.28 (1H, s), 8.34 (1H, d, J=8.8 Hz), 6.55 (1H, d, J=9.6 Hz), 5.79 (1H, d, J=1.2 Hz), 5.22-5.14 (2H, m), 5.04-5.00 (1H, m), 4.29-4.20 (2H, m), 4.17-4.06 (4H, m), 4.04-3.89 (2H, m), 3.60 (2H, s), 3.39-3.30 (1H, m), 3.10-3.03 (1H, m), 1.82 (3H, s), 1.72-1.60 (1H, m), 1.59-1.50 (1H, m), 1.39 (9H, s), 1.37 (9H, s), 1.27 (3H, s), 1.25-1.20 (6H, m); ¹³C NMR (100 MHz, CDCl₃) δ 171.1, 162.9, 161.4, 157.0, 156.0, 152.7, 145.4, 109.5, 108.7, 83.7, 79.6, 77.7, 74.9, 69.7, 65.8, 61.5, 59.5, 49.2, 47.9, 38.3, 31.7, 28.2 (3×), 28.0 (3×), 26.4, 25.3, 23.0, 14.1; ESI-HRMS calcd for C₃₁H₅₂N₅O₁₃: 702.3562, found: m/z 702.3560 [M+H]⁺.

5-Acetylamino-6-[2,3-dihydroxy-1-(3-hydroxypropylcarbamoyloxy)propyl]-4-guanidino-5,6-dihydro-4H-pyran-2-carboxylic acid (6)

To a solution of carbamate 16 (100 mg, 0.14 mmol) in THF (2 mL) was added 1 M KOH (2 mL). The solution was stirred at room temperature for 1.5 h, neutralized by Dowex 50W×8 (H⁺), filtered and concentrated under reduced pressure. The residue was dissolved in CH₂Cl₂ (2 mL) and TFA (2 mL). After stirring at room temperature for 3H, the mixture was evaporated under reduced pressure. The residue was triturated with Et₂O, and centrifuged to give the ZA-7-HP conjugate 6 (51 mg, 84%). C₁₆H₂₇N₅O₉; colorless solid, mp 265-267° C.; [α]²⁰ _(D) −218.7 (c=1.0, H₂O); IR ν_(max) (neat) 3410, 1674, 1544, 1263, 1140 cm⁻¹; ¹H NMR (400 MHz, D₂O) δ 6.05 (1H, d, J=2.4 Hz), 4.99 (1H, dd, J=8.8, 2.0 Hz), 4.60 (1H, dd, J=10.0, 2.0 Hz), 4.48 (1H, dd, J=10.0, 2.0 Hz), 4.18 (1H, dd, J=9.6, 9.2 Hz), 4.08-4.03 (1H, m), 3.72-3.63 (3H, m), 3.56-3.51 (1H, m), 3.17 (1H, dd, J=6.8, 6.8 Hz), 2.00 (3H, s), 1.78-1.71 (3H, m); ¹³C NMR (100 MHz, D₂O) δ 173.9, 164.8, 157.0, 156.6, 145.0, 109.1, 75.8, 69.5, 68.8, 62.3, 59.1, 51.1, 47.1, 37.5, 31.2, 21.8; ESI-HRMS (negative mode) calcd for C₁₆H₂₆N₅O₉: 432.1731, found: m/z 432.1727 [M−H]⁻.

Example 15 Synthesis of ZA-7-CA Conjugate 1 (FIG. 5) 3-(3,4-Bis-allyloxyphenyl)acrylic acid allyl ester (17)

To a solution of caffeic acid (1000 mg, 5.56 mmol) and ally bromide (2 mL, 22.2 mmol) in anhydrous acetone (15 ml) was added finely powdered K₂CO₃ (3069 mg, 22.2 mmol). The mixture was stirred for 4 h at 60° C., and then concentrated under reduced pressure. The residue was partitioned between EtOAc and 1 M HCl. The organic layer was washed with saturated NaHCO₃ and brine, dried over MgSO₄, filtered, and concentrated by rotary evaporation under reduced pressure. The crude material was purified by silica gel column chromatography (EtOAc/hexane=1:9) to yield a fully allylation product 17 (1320 mg, 79%). C₁₈H₂₀O₄; TLC (EtOAc/hexane=1:9) R_(f)=0.80; ¹H NMR (400 MHz, CDCl₃) δ 7.60 (1H, d, J=16.0 Hz), 7.05 (2H, m), 6.84 (1H, d, J=8.8 Hz), 6.27 (1H, d, J=16.0 Hz), 6.12-5.90 (3H, m), 5.42-5.25 (6H, m), 4.65 (2H, dd, J=5.6, 1.0 Hz), 4.60 (4H, dd, J=3.6, 1.8 Hz).

3-(3,4-Bis-allyloxyphenyl)acrylic acid (18)

To a solution of the fully allylation derivative of caffeic acid 17 (845 mg, 2.82 mmol) in MeOH (20 mL) was added 1M NaOH (2 mL). The mixture was stirred for 4 h at 60° C., and then concentrated under reduced pressure. H₂O (20 mL) was added and washed with ether, acidified with 1M HCl, then extracted with CH₂Cl₂. The organic layer was dried over MgSO₄, filtered, and concentrated by rotary evaporation under reduced pressure. The crude material was purified by silica gel column chromatography (EtOAc/hexane=3:7) to yield the bis-allyl ether 18 (695 mg, 95%). C₁₅H₁₆O₄; yellow solid; TLC (EtOAc/hexane=3:7); ¹H NMR (400 MHz, CDCl₃) δ 7.68 (1H, d, J=16.0 Hz), 7.08 (2H, m), 6.86 (1H, d, J=8.0 Hz), 6.26 (1H, d, J=16.0 Hz), 6.09-6.03 (2H, m), 5.44-5.27 (4H, m), 4.63 (4H, dd, J=3.2, 1.6 Hz); ¹³C NMR (100 MHz, CDCl₃) 172.5, 151.0, 148.5, 146.9, 133.0, 132.8, 127.2, 123.1, 118.0, 117.9, 114.9, 113.3, 112.8, 69.9, 69.7; ESI-HRMS (negative mode) calcd for C₁₅H₁₅O₄: 259.0970, found: m/z 259.0957 [M−H]⁻.

Compound 19

To a solution of ZA-7-HP derivative 16 (210 mg, 0.30 mmol) in CH₂Cl₂ (10 mL) was added the allyl ether of caffeic acid 18 (86 mg, 0.33 mmol), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDCI, 63 mg, 0.33 mmol) and 4-dimethylaminopyridine (DMAP, 37 mg, 0.33 mmol). The mixture was stirred at room temperature for 1.5 h. The resulting solution was extracted with 1 M HCl, saturated NaHCO₃ and brine, dried over MgSO₄, concentrated under reduced pressure, and purified by flash silica gel column chromatography (EtOAc/hexane=2:3 to EtOAc/hexane=3:2) to afford ester 19 (240 mg, 85%). C₄₆H₆₅N₅O₁₆; colorless solid, mp 98-100° C.; TLC (EtOAc/hexane=3:2) R_(f)=0.30; [α]²⁴ _(D) −20.3 (c=1.0, CHCl₃); IR ν_(max) (neat) 3316, 2980, 1728, 1638, 1609, 1510, 1306, 1253, 1142 cm⁻¹; ¹H NMR (400 MHz, CDCl₃) δ 11.36 (1H, s), 8.37 (1H, d, J=8.4 Hz), 7.54 (1H, d, J=16.0 Hz), 7.03 (2H, m), 6.82 (1H, d, J=8.8 Hz), 6.21 (1H, d, J=16.0 Hz), 6.16 (1H, d, J=9.6 Hz), 6.08-5.96 (2H, m), 5.83 (1H, d, J=2.0 Hz), 5.40-5.38 (1H, m), 5.36-5.34 (1H, m), 5.26-5.16 (4H, m), 5.10-5.06 (1H, m), 4.59 (4H, dd, J=2.8, 2.4 Hz), 4.36-4.28 (2H, m), 4.24-4.16 (3H, m), 4.09-3.95 (4H, m), 3.28-3.19 (2H, m), 1.84 (5H, s), 1.42 (9H, s), 1.41 (9H, s), 1.34 (3H, s), 1.30-1.18 (6H, m); ¹³C NMR (100 MHz, CDCl₃) δ 170.8, 167.3, 163.0, 161.4, 157.0, 155.6, 152.7, 150.6, 148.5, 145.4, 144.8, 133.0, 132.8, 127.5, 122.7, 117.9, 117.8, 115.6, 113.4, 112.6, 109.5, 108.7, 83.6, 79.6, 77.5, 74.9, 69.9, 69.7, 65.9, 61.6, 61.5, 48.7, 48.4, 37.9, 36.6, 29.6, 29.0, 28.2 (3×), 28.0 (3×), 25.3, 23.0, 14.1; ESI-HRMS calcd for C₄₆H₆₆N₅O₁₆: 944.4505, found: m/z 944.4518 [M+H]⁺.

ZA-7-CA Conjugate 1

To a solution of compound 19 (215 mg, 0.23 mmol) in THF (4 mL) was added 1 M KOH (4 mL). The solution was stirred at room temperature for 1.5 h, neutralized by Dowex 50W×8 (H⁺), filtered and concentrated under reduced pressure. The residue was dissolved in anhydrous THF (5 mL), then added Pd(PPh₃)₄ (26 mg, 0.02 mmol) and morpholine (0.4 mL, 4.5 mmol). The mixture was stirred at room temperature for 4 h. The resulting solution was extracted with 1 M HCl and brine, dried over MgSO₄, concentrated under reduced pressure, and purified by flash silica gel column chromatography (MeOH/CH₂Cl₂=1:9 to MeOH/CH₂Cl₂=1:4). The residue was dissolved in CH₂Cl₂ (2 mL) and TFA (2 mL). After stirring at room temperature for 3H, the mixture was evaporated under reduced pressure. The residue was triturated with Et₂O, and centrifuged to give ZA-7-CA conjugate 1 (75 mg, 56%). C₂₅H₃₃N₅O₁₂; yellow solid, mp 217-219° C.; [α]²⁴ _(D) +144.9 (c=0.33, H₂O); IR ν_(max) (neat) 3403, 1702, 1628, 1404, 1262 cm⁻¹; ¹H NMR (400 MHz, CD₃OD) δ 7.55 (1H, d, J=16.0 Hz), 7.04 (1H, d, J=1.6 Hz), 6.94 (1H, dd, J=8.4, 2.0 Hz), 6.78 (1H, d, J=8.4 Hz), 6.26 (1H, d, J=16.0 Hz), 5.84 (1H, d, J=2.4 Hz), 5.00 (1H, dd, J=8.8, 2.0 Hz), 4.57 (1H, dd, J=10.0, 2.0 Hz), 4.43 (1H, dd, J=6.8, 1.6 Hz), 4.25-4.16 (2H, m), 4.04-3.99 (1H, m), 3.89-3.86 (1H, m), 3.66 (1H, dd, J=12.0, 3.2 Hz), 3.53-3.47 (1H, m), 3.23-3.19 (2H, m), 1.95 (3H, s), 1.92-1.86 (2H, m); ¹³C NMR (100 MHz, CD₃OD) δ 172.3, 168.0, 163.7, 157.5, 156.6, 148.2, 145.8, 145.5, 145.4, 126.3, 121.6, 115.1, 113.7 (2×), 107.1, 75.9, 69.5, 69.1, 62.9, 61.6, 51.1, 48.5, 37.3, 28.6, 21.4; ESI-HRMS (negative mode) calcd for C₂₅H₃₂N₅O₁₂: 594.2047, found: m/z 594.2051 [M−H]⁻.

Example 16 Synthesis of ZA-7-MCA Conjugate 2 (FIG. 6) Compound 20

To a solution of ZA-7-HP derivative 16 (90 mg, 0.13 mmol) in CH₂Cl₂ (5 mL) were added 3,4-(methylenedioxy)cinnamic acid (MCA, 27 mg, 0.14 mmol), EDCI (27 mg, 0.14 mmol) and 4-dimethylaminopyridine (17 mg, 0.14 mmol). The mixture was stirred at room temperature for 1.5 h, and then was extracted with 1 M HCl, saturated NaHCO₃ and brine. The organic phase was dried over MgSO₄, concentrated under reduced pressure, and purified by flash silica gel column chromatography (EtOAc/hexane=3:7 to EtOAc/hexane=1:1) to afford ester 20 (98 mg, 86%). C₄₁H₅₇N₅O₁₆; colorless solid, mp 112-114° C.; TLC (EtOAc/hexane=3:2) R_(f)=0.18; [α]²⁵ _(D) −19.2 (c=1.0, CH₃C1); IR ν_(max) (neat) 3316, 2980, 2931, 1729, 1639, 1609, 1250, 1152 cm⁻¹; ¹H NMR (400 MHz, CDCl₃) δ 11.34 (1H, s), 8.34 (1H, d, J=8.4 Hz), 7.51 (1H, d, J=16.0 Hz), 6.97 (1H, s), 6.94 (1H, d, J=8.0 Hz), 6.73 (1H, d, J=8.0 Hz), 6.30 (1H, d, J=9.2 Hz), 6.19 (1H, d, J=16.0 Hz), 5.94 (2H, s), 5.82 (1H, d, J=2.4 Hz), 5.24-5.11 (3H, m), 4.33-4.72 (2H, m), 4.22-4.16 (4H, m), 4.14-4.04 (2H, m), 4.02-3.95 (1H, m), 3.22-3.19 (2H, m), 1.84 (5H, s), 1.41 (9H, s), 1.40 (9H, s), 1.31 (3H, s), 1.28-1.23 (6H, m); ¹³C NMR (100 MHz, CDCl₃) δ 170.6, 167.1, 162.9, 161.3, 156.8, 155.5, 152.4, 149.5, 148.2, 145.3, 144.5, 128.6, 124.4, 115.7, 109.6, 108.6, 108.4, 106.4, 101.4, 83.4, 79.4, 77.5, 74.9, 69.6, 65.7, 61.6, 61.4, 48.7, 48.1, 37.8, 28.9, 28.1 (3×), 27.9 (3×), 26.3, 25.2, 22.9, 14.0; ESI-HRMS calcd for C₄₁H₅₈N₅O₁₆: 876.3879, found: m/z 876.3876 [M+H]⁺.

ZA-7-MCA Conjugate 2

To a solution of compound 20 (98 mg, 0.11 mmol) in THF (2 mL) was added 1 M KOH (2 mL). The solution was stirred at room temperature for 1.5 h, neutralized by Dowex 50W×8 (H⁺), filtered and concentrated under reduced pressure. The residue was dissolved in CH₂Cl₂ (2 mL) and TFA (2 mL). After stirring at room temperature for 3H, the mixture was evaporated under reduced pressure. The residue was triturated with Et₂O, and centrifuged to give the ZA-7-MCA conjugate 2 (48 mg, 71%). C₂₆H₃₃N₅O₁₂; white solid, mp 218-220° C.; [α]²⁰ _(D)+11.4 (c=0.33, H₂O); IR ν_(max) (neat) 3369, 2920, 1686, 1629, 1449, 1252, 1038 cm⁻¹; ¹H NMR (400 MHz, CD₃OD) δ 7.60 (1H, d, J=16.0 Hz), 7.14 (1H, s), 7.10 (1H, d, J=8.0 Hz), 6.87 (1H, d, J=8.0 Hz), 6.35 (1H, d, J=16.0 Hz), 6.01 (2H, s), 5.59 (1H, d, J=2.0 Hz), 4.93 (1H, dd, J=7.6, 1.6 Hz), 4.48 (1H, dd, J=10.4, 1.6 Hz), 4.36 (1H, dd, J=9.2, 2.0 Hz), 4.26-4.14 (3H, m), 4.08-4.03 (1H, m), 3.63 (1H, dd, J=12.0, 2.8 Hz), 3.51-3.46 (1H, m), 3.21 (2H, t, J=6.4 Hz), 1.95 (3H, s), 1.90 (2H, t, J=6.8 Hz); ¹³C NMR (100 MHz, CD₃OD) δ 174.0, 169.7, 158.7, 158.1, 151.4, 151.3, 149.8, 146.6, 146.4, 130.0, 126.1, 126.0, 116.5, 109.7, 107.5, 103.2, 103.1, 76.9, 71.2, 70.2, 64.4, 63.5, 53.4, 38.8, 29.8, 23.1; ESI-HRMS (negative mode) calcd for C₂₆H₃₂N₅O₁₂: 606.2047, found: m/z 606.2044 [M−H]⁻.

Example 17 Synthesis of ZA-7-ME Conjugate 3 (FIG. 7) 2-Allyloxy-5-tert-butoxycarbonylamino-benzoic acid allyl ester (21)

To a stirred solution of mesalazine (500 mg, 3.27 mmol) in CH₂Cl₂ (10 ml) was added Et₃N (0.9 mL, 6.54 mmol), followed by Boc₂O (784 mg, 3.60 mmol). The mixture was stirred at room temperature for 2H, and then quenched by adding saturated NaHCO₃. The mixture was poured into water and extracted with EtOAc (3×). The combined organic layer was dried over MgSO₄, filtered, and concentrated under reduced pressure to give the N-Boc protected derivative, 5-tert-butoxycarbonylamino-2-hydroxybenzoic acid (803 mg, 97%). The crude product was used in the next reaction without further purification. Pale yellow solid; C₁₂H₁₅NO₅; ¹H NMR (400 MHz, CD₃OD) δ 7.92 (1H, d, J=2.0 Hz), 7.44 (1H, d, J=8.8 Hz), 6.84 (1H, d, J=8.8 Hz), 1.51 (9H, s); ¹³C NMR (100 MHz, CD₃OD) δ 172.6, 158.3, 155.0, 131.3, 128.0, 121.2, 117.5, 112.8, 80.2, 28.0 (3×); ESI-HRMS (negative mode) calcd for C₁₂H₁₄NO₅: 252.0872, found: m/z 252.0862 [M−H]⁻.

To a solution of the above-prepared N-Boc protected derivative (605 mg, 2.39 mmol) in anhydrous acetone (10 mL) were added ally bromide (0.6 mL, 7.17 mmol) and finely powdered K₂CO₃ (990 mg, 7.17 mmol). The mixture was stirred for 4 h at 60° C., and then concentrated under reduced pressure. The residue was partitioned between EtOAc and 1 M HCl. The organic layer was washed with saturated NaHCO₃ and brine, dried over MgSO₄, filtered, and concentrated by rotary evaporation under reduced pressure. The crude material was purified by silica gel column chromatography (EtOAc/hexane=2:8) to yield an allylation product 21 (620 mg, 78%). C₁₈H₂₃NO₅; white solid, mp 77-78° C.; ¹H NMR (400 MHz, CDCl₃) δ 7.72 (1H, d, J=2.8 Hz), 7.50 (1H, br), 7.18 (1H, s), 6.76 (1H, d, J=9.2 Hz), 5.94-5.85 (2H, m), 5.37-5.27 (2H, m), 5.18-5.11 (2H, m), 4.68 (2H, m), 4.34 (2H, m), 1.38 (9H, s); ¹³C NMR (100 MHz, CDCl₃) δ 165.4, 153.6, 153.0, 132.6, 131.9, 131.5, 124.1, 122.1, 120.2, 117.8, 117.0, 114.3, 79.8, 69.7, 65.2, 28.0 (3×); ESI-HRMS (negative mode) calcd for C₁₈H₂₂NO₅: 332.1498 found: m/z 332.1499 [M−H]⁻.

2-Allyloxy-5-tert-butoxycarbonylamino-benzoic acid (22)

To a solution of compound 21 (500 mg, 1.50 mmol) in MeOH (20 mL) was added 1 M NaOH (2 mL). The mixture was stirred for 4 h at 60° C., and then concentrated under reduced pressure. H₂O (20 mL) was added and washed with ether, acidified with 1 M HCl, then extracted with CH₂Cl₂. The organic layer was dried over MgSO₄, filtered, and concentrated by rotary evaporation under reduced pressure. The crude material was purified by silica gel column chromatography (EtOAc/hexane=2:3) to yield the allyl ether 22 (405 mg, 92%). C₁₅H₁₉NO₅; yellow solid; [α]²² _(D) +2.1 (c=0.5, CHCl₃); IR ν_(max) (neat) 3347, 1694, 1589, 1523, 1416, 1260, 1239, 1163 cm⁻¹; ¹H NMR (400 MHz, CDCl₃) δ 7.71 (1H, d, J=2.8 Hz), 7.50 (1H, s), 7.18 (1H, s), 6.86 (1H, d, J=8.8 Hz), 6.05-5.96 (2H, m), 6.76 (1H, d, J=9.2 Hz), 5.43 (1H, dt, J=17.2, 1.6 Hz), 5.23 (1H, dt, J=10.4, 1.6 Hz), 4.54 (2H, m), 1.47 (9H, s); ¹³C NMR (100 MHz, CDCl₃) 165.9, 152.8, 133.0, 131.1, 125.5, 123.2, 119.6, 117.8, 113.7, 80.2, 77.2, 70.7, 28.0 (3×); ESI-HRMS (negative mode) calculated for C₁₅H₁₈NO₅: 292.1185 found: m/z 292.1179 [M−H]⁻.

Compound 23

To a solution of ZA-7-HP derivative 16 (70 mg, 0.10 mmol) in CH₂Cl₂ (5 mL) was added the mesalamine derivative 22 (33 mg, 0.11 mmol), EDCI (21 mg, 0.11 mmol) and 4-dimethylaminopyridine (13 mg, 0.11 mmol). The mixture was stirred at room temperature for 1.5 h. The resulting solution was extracted with 1 M HCl, saturated NaHCO₃ and brine, dried over MgSO₄, concentrated under reduced pressure, and purified by flash silica gel column chromatography (EtOAc/hexane=3:7 to EtOAc/hexane=1:1) to afford ester 23 (75 mg, 77%). C₄₆H₆₈N₆O₁₇; light yellow solid, mp 114-116° C.; TLC (EtOAc/hexane=7:3) R_(f)=0.33; [α]²⁰ _(D)−11.5 (c=1.0, CH₃Cl); IR ν_(max) (neat) 3319, 2979, 1728, 1610, 1248, 1154 cm⁻¹; ¹H NMR (400 MHz, CDCl₃) δ 11.32 (1H, s), 8.29 (1H, d, J=8.8 Hz), 7.70 (1H, br), 7.57 (1H, d, J=2.4 Hz), 7.00 (1H, br), 6.87 (1H, d, J=9.2 Hz), 6.15 (1H, d, J=9.2 Hz), 6.07-5.97 (1H, m), 5.79 (1H, d, J=2.0 Hz), 5.42 (1H, s), 5.38 (1H, s), 5.26-5.16 (2H, m), 4.56 (1H, d, J=4.8 Hz), 4.42-4.19 (7H, m), 4.09-3.89 (4H, m), 3.35-3.24 (2H, m), 1.98-1.92 (2H, m), 1.85 (3H, s), 1.46 (9H, s), 1.43 (9H, s), 1.42 (9H, s), 1.32 (3H, s), 1.31-1.25 (6H, m); ¹³C NMR (100 MHz, CD Cl₃) δ 170.8, 166.2, 163.1, 161.5, 156.9, 155.6, 153.8, 153.1, 152.8, 145.4, 132.9, 131.8, 124.4, 122.3, 120.4, 117.9, 114.6, 109.6, 108.8, 83.7, 80.3, 79.6, 74.9, 70.1, 69.6, 65.8, 63.1, 61.5, 48.7, 48.4, 38.7, 29.7, 28.5, 28.3 (3×), 28.2 (3×), 28.0 (3×), 26.5, 25.4, 23.0, 14.1; ESI-HRMS calcd for C₄₆H₆₉N₆O₁₇: 977.4719, found: m/z 977.4716 [M+H]⁺.

ZA-7-ME Conjugate 3

To a solution of compound 23 (70 mg, 0.07 mmol) in THF (4 mL) was added 1 M KOH (4 mL). The solution was stirred at room temperature for 1.5 h, neutralized by Dowex® 50W×8 (H⁺), filtered and concentrated under reduced pressure. The residue was dissolved in anhydrous THF (5 mL), then added Pd(PPh₃)₄ (12 mg, 0.01 mmol) and morpholine (0.1 mL, 1.4 mmol). The mixture was stirred at room temperature for 4 h. The resulting solution was extracted with 1 M HCl and brine, dried over MgSO₄, concentrated under reduced pressure, and purified by flash silica gel column chromatography (MeOH/CH₂Cl₂=1:20 to MeOH/CH₂Cl₂=1:9). The residue was dissolved in CH₂Cl₂ (2 mL) and TFA (2 mL). After stirring at room temperature for 3H, the mixture was evaporated under reduced pressure. The residue was triturated with Et₂O, and centrifuged to give ZA-7-ME conjugate 3 (28 mg, 69%). C₂₃H₃₂N₆O₁₁; orange solid, mp 210-212° C.; [α]²² _(D) −53.0 (c=0.5, MeOH); IR ν_(max) (neat) 3351, 1673, 1622, 1493, 1403, 1228 cm⁻¹; ¹H NMR (400 MHz, D₂O) δ 7.38 (1H, d, J=2.8 Hz), 7.10 (1H, dd, J=8.8, 2.8 Hz), 6.88 (1H, d, J=8.8 Hz), 5.67 (1H, d, J=2.0 Hz), 4.93 (1H, dd, J=9.2, 1.6 Hz), 4.50 (1H, dd, J=10.4, 1.6 Hz), 4.47-4.38 (3H, m), 4.09-4.01 (2H, m), 3.64 (1H, dd, J=12.0, 2.8 Hz), 3.48-3.43 (1H, m), 3.32-3.28 (2H, m), 2.00-1.96 (5H, m); ¹³C NMR (100 MHz, D₂O) δ 173.6, 169.8, 168.6, 157.0, 156.6, 153.6, 149.4, 136.9, 125.8, 117.8, 117.3, 112.9, 104.5, 75.2, 69.8, 68.5, 63.6, 62.5, 51.9, 47.3, 37.8, 27.8, 21.9; ESI-HRMS calcd for C₂₃H₃₃N₆O₁₁: 569.2207, found: m/z 569.2215 [M+H]⁺.

Example 18 Synthesis of ZA-7-HNAP Conjugate 4 (FIG. 8) Allyl ether allyl ester of 1-hydroxy-2-naphthoic acid (24)

To a solution of 1-hydroxy-2-napthoic acid (HNAP, 1.04 g, 5.51 mmol) in anhydrous acetone (12 mL) were added ally bromide (1.4 mL, 16.53 mmol) and finely powdered K₂CO₃ (2.28 g, 16.53 mmol). The mixture was stirred for 4 h at 60° C., and then concentrated under reduced pressure. The residue was partitioned between EtOAc and 1 M HCl. The organic layer was washed with saturated NaHCO₃ and brine, dried over MgSO₄, filtered, and concentrated by rotary evaporation under reduced pressure. The crude material was purified by silica gel column chromatography (EtOAc/hexane=1:9) to yield the bis-allylation product 24 (1180 mg, 80%). C₁₇H₁₆O₃; TLC (EtOAc/hexane=1:9) R_(f)=0.65; IR ν_(max) (neat) 1722, 1334, 1274, 1233, 1131 cm⁻¹; ¹H NMR (400 MHz, CDCl₃) δ 8.27-8.24 (1H, m), 7.87 (1H, d, J=8.4 Hz), 7.77-7.27 (1H, m), 7.53 (1H, d, J=8.4 Hz), 7.51-7.47 (2H, m), 6.24-6.17 (1H, m), 6.09-6.02 (1H, m), 5.50-5.40 (2H, m), 5.30-5.25 (2H, m), 4.87-4.84 (2H, m), 4.61-4.62 (2H, m); ¹³C NMR (100 MHz, CDCl₃) δ 165.4, 156.7, 136.4, 133.5, 131.9, 128.5, 128.0, 127.5, 126.3, 126.2, 123.4, 123.3, 119.3, 118.2, 117.4, 76.3, 65.4; ESI-HRMS calcd for C₁₇H₁₇O₃: 269.1178, found: m/z 269.1184 [M+H]⁺.

Allyl ether of 1-hydroxy-2-naphthoic acid (25)

To a solution of the allyl ester 24 (350 mg, 1.31 mmol) in MeOH (10 mL) was added 1 M NaOH (1 mL). The mixture was stirred for 4 h at 60° C., and then concentrated under reduced pressure. H₂O (20 mL) was added and washed with ether, acidified with 1 M HCl, then extracted with CH₂Cl₂. The organic layer was dried over MgSO₄, filtered, and concentrated by rotary evaporation under reduced pressure. The crude material was purified by silica gel column chromatography (EtOAc/hexane=3:7) to yield the allyl ether 25 of HNAP (289 mg, 97%). C₁₄H₁₂O₃; yellow solid, mp 99-101° C.; TLC (EtOAc/hexane=3:7) R_(f)=0.18; IR ν_(max) (neat) 3455, 1738, 1365, 1232, 1228 cm⁻¹; ¹H NMR (400 MHz, CDCl₃) δ 8.21 (1H, d, J=9.6 Hz), 8.03 (1H, d, J=8.4 Hz), 7.83 (1H, dd, J=8.4, 1.2 Hz), 7.63 (1H, d, J=8.8 Hz), 7.60-7.52 (2H, m), 6.28-6.17 (1H, m), 5.50 (1H, dd, J=17.2, 1.2 Hz), 5.37 (1H, dd, J=10.4, 0.8 Hz), 4.71 (1H, d, J=6.0 Hz); ¹³C NMR (100 MHz, CDCl₃) δ 169.2, 157.1, 137.2, 132.5, 128.8, 128.0, 127.8, 126.7, 126.6, 124.3, 119.2, 118.2, 77.4; ESI-HRMS (negative mode) calcd for C₁₄H₁₁O₃: 227.0708, found: m/z 227.0715 [M−H]⁻.

Compound 26

To a solution of ZA-7-HP derivative 16 (96 mg, 0.14 mmol) in CH₂Cl₂ (5 mL) was added mesalamine derivative 25 (34 mg, 0.15 mmol), EDCI (29 mg, 0.15 mmol) and 4-dimethylaminopyridine (18 mg, 0.15 mmol). The mixture was stirred at room temperature for 1.5 h. The resulting solution was extracted successively with 1 M HCl, saturated NaHCO₃ and brine, dried over MgSO₄, concentrated under reduced pressure, and purified by flash silica gel column chromatography (EtOAc/hexane=1:4 to EtOAc/hexane=1:1) to afford compound 26 (101 mg, 82%). C₄₅H₆₁N₅O₁₅; white solid, mp 95-97° C.; TLC (EtOAc/hexane=1:1) R_(f)=0.62; [α]²⁰ _(D) −17.0 (c=1.0, CHCl₃); IR ν_(max) (neat) 3316, 2979, 2930, 1724, 1641, 1610, 1369, 1249, 1141 cm⁻¹; ESI-HRMS calcd for C₄₅H₆₂N₅O₁₅: 912.4242, found: m/z 912.4249 [M+H]⁺.

ZA-7-HNAP Conjugate 4

To a solution of compound 26 (84 mg, 0.09 mmol) in THF (3 mL) was added 1 M KOH (3 mL). The solution was stirred at room temperature for 1.5 h, neutralized by Dowex 50W×8 (H⁺), filtered and concentrated under reduced pressure. The residue was dissolved in anhydrous THF (5 mL), then added Pd(PPh₃)₄ (12 mg, 0.01 mmol) and morpholine (0.24 mL, 2.3 mmol). The mixture was stirred at room temperature for 4 h. The resulting solution was extracted with 1 M HCl and brine, dried over MgSO₄, concentrated under reduced pressure, and purified by flash silica gel column chromatography (MeOH/CH₂Cl₂=1:9 to MeOH/CH₂Cl₂=1:4). The residue was dissolved in CH₂Cl₂ (2 mL) and TFA (2 mL). After stirring at room temperature for 3H, the mixture was evaporated under reduced pressure. The residue was triturated with Et₂O, and centrifuged to give ZA-7-HNAP conjugate 4 (30 mg, 55%). C₂₇H₃₃N₅O₁₁; colorless solid, mp 230-232° C.; [α]²⁴ _(D) −5.0 (c=0.5, CH₃OH); IR ν_(max) (neat) 3402, 1740, 1655, 1596, 1406, 1369, 1256, 1163 cm⁻¹; ¹H NMR (400 MHz, CD₃OD) δ 8.34 (1H, d, J=8.4 Hz), 7.80 (2H, d, J=8.8 Hz), 7.61 (1H, m), 7.51 (1H, t, J=7.6 Hz), 7.31 (1H, d, J=8.8 Hz), 5.58 (1H, d, J=2.0 Hz), 4.97 (1H, m), 4.49-4.44 (3H, m), 4.34 (1H, d, J=8.8 Hz), 4.23-4.18 (1H, m), 4.07-4.02 (1H, m), 3.64 (1H, dd, J=12.0, 2.4 Hz), 3.51-3.46 (1H, m), 3.30-3.27 (2H, m), 2.04-1.97 (2H, m), 1.93 (3H, s); ¹³C NMR (100 MHz, CD₃OD) δ 172.1, 170.9, 160.4, 157.5, 156.8, 137.3, 129.1, 127.3, 125.5, 124.5, 124.0, 123.1, 118.4, 105.5, 103.3, 75.7, 69.8, 68.8, 63.1, 62.8, 52.0, 47.8, 37.3, 29.3, 28.5, 21.5; ESI-HRMS (negative mode) calcd for C₂₇H₃₂N₅O₁₁: 602.2098, found: m/z 602.2087 [M−H]⁻.

Example 19 Synthesis of ZA-7-DHBA Conjugate 5 (FIG. 9) 3,4-(Bis-allyloxy)benzoic acid allyl ester (27)

To a solution of 3,4-dihydroxybenzoic acid (DHBA, 676 mg, 4.39 mmol) in anhydrous acetone (10 ml) were added ally bromide (1.5 mL, 17.56 mmol) and finely powdered K₂CO₃ (2426 mg, 17.56 mmol). The mixture was stirred for 4 h at 60° C., and then concentrated under reduced pressure. The residue was partitioned between EtOAc and 1 M HCl. The organic layer was washed with saturated NaHCO₃ and brine, dried over MgSO₄, filtered, and concentrated by rotary evaporation under reduced pressure. The crude material was purified by silica gel column chromatography (EtOAc/hexane=1:9) to yield a fully allylation product 27 (852 mg, 71%). C₁₆H₁₈O₄; yellow oil; TLC (EtOAc/hexane=1:9) R_(f)=0.52; ¹H NMR (400 MHz, CDCl₃) δ 7.63 (1H, dd, J=8.4, 1.6 Hz), 7.55 (1H, d, J=1.6 Hz), 6.84 (1H, d, J=8.4 Hz), 6.01-5.92 (3H, m), 5.43-5.33 (3H, m), 5.27-5.21 (3H, m), 4.75 (2H, d, J=5.6 Hz), 4.62-4.60 (4H, m); ¹³C NMR (100 MHz, CDCl₃) δ 165.2, 152.5, 147.9, 133.0, 132.7, 132.4, 123.8, 122.7, 118.0 (2×), 117.9, 114.6, 112.4, 69.8, 69.6, 65.3; ESI-HRMS calcd for C₁₆H₁₉O₄: 275.1283, found: m/z 275.1293 [M+H]⁺.

3,4-(Bis-allyloxy)benzoic acid (28)

To a solution of the allyl ester 27 (300 mg, 1.09 mmol) in MeOH (5 mL) was added 1 M NaOH (0.5 mL). The mixture was stirred for 4 h at 60° C., and then concentrated under reduced pressure. H₂O (15 mL) was added and washed with ether, acidified with 1 M HCl, then extracted with CH₂Cl₂. The organic layer was dried over MgSO₄, filtered, and concentrated by rotary evaporation under reduced pressure. The crude material was purified by silica gel column chromatography (EtOAc/hexane=3:7) to yield acid 28 (235 mg, 92%). C₁₃H₁₄O₄; colorless solid; TLC (EtOAc/hexane=3:7) R_(f)=0.35; ¹H NMR (400 MHz, CDCl₃) δ 7.71 (1H, dd, J=8.8, 1.0 Hz), 7.59 (1H, d, J=2.0 Hz), 6.89 (1H, d, J=8.8 Hz), 6.12-6.01 (2H, m), 5.45-5.39 (2H, m), 5.31-5.27 (2H, m), 4.66-4.63 (4H, m); ¹³C NMR (100 MHz, CDCl₃) δ172.0, 153.1, 147.8, 132.8, 132.5, 124.6, 121.8, 118.1, 118.0, 114.8, 112.3, 69.8, 69.6; ESI-HRMS (negative mode) calcd for C₁₃H₁₃O₄: 233.0814, found: m/z 233.0812 [M−H]⁻.

Compound 29

To a solution of ZA-7-HP derivative 16 (100 mg, 0.14 mmol) in CH₂Cl₂ (5 mL) was added 3,4-(bis-allyloxy)benzoic acid 28 (37 mg, 0.16 mmol), EDCI (30 mg, 0.16 mmol) and 4-dimethylaminopyridine (20 mg, 0.16 mmol). The mixture was stirred at room temperature for 1.5 h. The resulting solution was extracted with 1 M HCl, saturated NaHCO₃ and brine, dried over MgSO₄, concentrated under reduced pressure, and purified by flash silica gel column chromatography (EtOAc/hexane=3:7 to EtOAc/hexane=3:2) to afford compound 29 (90 mg, 70%). C₄₄H₆₃N₅O₁₆; white solid, mp 102-104° C.; [α]²⁰ _(D) −26.5 (c=0.5, CHCl₃); IR ν_(max) (neat) 3315, 2980, 2931, 1729, 1641, 1608, 1267, 1141 cm⁻¹; ¹H NMR (400 MHz, CDCl₃) δ 11.36 (1H, s), 8.40 (1H, d, J=8.8 Hz), 7.59 (1H, d, J=8.4 Hz), 7.52 (1H, s), 6.85 (1H, d, J=8.8 Hz), 6.11 (1H, d, J=5.2 Hz), 6.08-5.98 (2H, m), 5.84 (1H, d, J=2.0 Hz), 5.40 (2H, dd, J=17.2, 4.8 Hz), 5.27-5.16 (5H, m), 5.10-5.07 (1H, m), 4.61 (1H, t, J=5.6 Hz), 4.33-4.31 (5H, m), 4.23-3.97 (6H, m), 3.28-3.25 (2H, m), 1.95-1.92 (2H, m), 1.86 (3H, s), 1.44 (9H, s), 1.42 (9H, s), 1.34 (3H, s), 1.30-1.26 (6H, m); ¹³C NMR (100 MHz, CDCl₃) δ 170.7, 166.3, 163.0, 161.4, 157.0, 155.6, 152.7, 152.5, 147.9, 145.5, 133.0, 132.7, 123.7, 122.7, 118.0, 117.9, 114.6, 112.5, 109.5, 108.7, 83.6, 79.5, 77.6, 74.9, 69.8, 69.6, 65.9, 62.0, 61.5, 48.9, 48.4, 38.0, 29.6, 29.1, 28.2 (3×), 28.0 (3×), 26.4, 25.3, 23.0, 14.1; ESI-HRMS (negative mode) calcd for C₄₄H₆₂N₅O₁₆: 916.4192, found: m/z 916.4194 [M−H]⁻.

ZA-7-DHBA Conjugate 5

To a solution of compound 29 (80 mg, 0.09 mmol) in THF (3 mL) was added 1 M KOH (3 mL). The solution was stirred at room temperature for 1.5 h, neutralized by Dowex 50W×8 (H⁺), filtered and concentrated under reduced pressure. The residue was dissolved in anhydrous THF (5 mL), then added Pd(PPh₃)₄ (23 mg, 0.02 mmol) and morpholine (0.4 mL, 4.5 mmol). The mixture was stirred at room temperature for 4 h. The resulting solution was extracted with 1 M HCl and brine, dried over MgSO₄, concentrated under reduced pressure, and purified by flash silica gel column chromatography (MeOH/CH₂Cl₂=1:9 to MeOH/CH₂Cl₂=1:4). The residue was dissolved in CH₂Cl₂ (2 mL) and TFA (2 mL). After stirring at room temperature for 3H, the mixture was evaporated under reduced pressure. The residue was triturated with Et₂O, and centrifuged to give ZA-7-DHBA conjugate 5 (26 mg, 52%). C₂₃H₃₁N₅O₁₂; yellow solid, mp 223-225° C.; [α]²⁵ _(D) −6.5 (c=0.33, H₂O); IR ν_(max) (neat) 3376, 1674, 1618, 1405, 1296, 1234, 1119 cm⁻¹; ¹H NMR (400 MHz, CD₃OD) δ 7.44 (1H, s), 7.42 (1H, d, J=8.0 Hz), 6.80 (1H, dd, J=8.0 Hz), 5.58 (1H, d, J=2.0 Hz), 4.95 (1H, m), 4.46 (1H, dd, J=10.4, 2.0 Hz), 4.36-4.29 (3H, m), 4.20-4.15 (1H, m), 4.06-4.01 (1H, m), 3.62 (1H, dd, J=12.0, 2.8 Hz), 3.49-3.45 (1H, m), 3.30-3.22 (2H, m), 1.94 (3H, s), 1.93-1.91 (2H, m); ¹³C NMR (100 MHz, CD₃OD) δ 173.7, 169.3, 168.5, 159.0, 158.3, 151.8, 151.7, 146.3, 123.8, 122.9, 117.6, 116.1, 104.6, 77.1, 71.3, 70.4, 64.6, 63.5, 39.0, 30.2, 23.0; ESI-HRMS (negative mode) calcd for C₂₃H₃₀N₅O₁₂: 568.1891, found: m/z 568.1893 [M−H]⁻.

Example 20 Synthesis of ZA-7-CA-Amide Conjugate 7 (FIG. 10) Tert-Butyl 3-aminopropyl carbamate (30)

A solution of Boc₂O (500 mg, 2.29 mmol) in 1,4-dioxane (10 mL) was added over 3 h to a stirring solution of 1,3-diaminopropane (2.5 mL, 11.45 mmol) in 1,4-dioxane (10 mL) at room temperature. After stirring for 16 h, the solvent was removed under reduced pressure. Water (20 mL) was added to the residue, and the insoluble side product was collected by filtration. The filtrate was extracted with CH₂Cl₂. The organic extract was dried over MgSO₄, filtered and concentrated under reduced pressure to afford carbamate 30 (358 mg, 90%). C₈H₁₈N₂O₂; colorless oil; ¹H NMR (400 MHz, CDCl₃) δ 5.32 (1H, br), 2.95 (2H, m), 2.51 (2H, t, J=6.4 Hz), 1.48 (1H, br), 1.38 (2H, t, J=6.4 Hz), 1.20 (9H, s); ¹³C NMR (100 MHz, CDCl₃) δ 155.9, 78.2, 39.0, 37.7, 32.9, 28.0; ESI-HRMS calcd for C₈H₁₉N₂O₂: 175.1447, found: m/z 175.1445 [M+H]⁺.

Tert-Butyl 3-[(3,4-Bis-allyloxyphenyl)acryloylamino]propyl carbamate (31)

To a solution of allyl ether of caffeic acid 18 (134 mg, 0.51 mmol) in CH₂Cl₂ (5 mL) was added amine 30 (98 mg, 0.56 mmol), EDCI (108 mg, 0.56 mmol) and 4-dimethylaminopyridine (68 mg, 0.56 mmol). The mixture was stirred at room temperature for 1.5 h. The resulting solution was extracted successively with 1 M HCl, saturated NaHCO₃ and brine, dried over MgSO₄, concentrated under reduced pressure, and purified by flash silica gel column chromatography (EtOAc/hexane=1:3 to EtOAc/hexane=2:3) to afford the coupling product 31 (154 mg, 73%). C₂₃H₃₂N₂O₅; colorless solid, mp 115-117° C.; [α]²⁴ _(D) −3.7 (c=0.25, CHCl₃); IR ν_(max) (neat) 3306, 2925, 1694, 1658, 1510, 1257 cm⁻¹; ¹H NMR (400 MHz, CDCl₃) δ 7.46 (1H, d, J=15.6 Hz), 6.97 (2H, m), 6.82 (1H, br), 6.76 (1H, d, J=8.0 Hz), 6.28 (1H, d, J=15.6 Hz), 6.03-5.95 (2H, m), 5.35 (2H, d, J=17.2 Hz), 5.23-5.20 (3H, m), 4.53 (4H, s), 3.35 (2H, t, J=5.6 Hz), 3.12 (2H, t, J=5.2 Hz), 1.61 (2H, s), 1.38 (9H, s); ¹³C NMR (100 MHz, CDCl₃) δ 166.5, 156.6, 149.8, 148.3, 140.3, 133.0, 132.8, 128.0, 121.8, 118.8, 117.7, 117.6, 113.4, 112.7, 79.1, 69.8, 69.6, 36.9, 35.9, 30.1, 28.2 (3×); ESI-HRMS calcd for C₂₃H₃₃N₂O₅: 417.2389, found: m/z 417.2388 [M+H]⁺.

Compound 33

To a solution of the above-prepared N-Boc compound 31 (150 mg, 0.36 mmol) in CH₂Cl₂ (2 mL) was added TFA (2 mL). After stirring at room temperature for 1 h, the mixture was evaporated under reduced pressure to give the corresponding amine 32. The crude amine product was dissolved in CH₃CN (7 mL), and added carbonate 15 (260 mL, 0.34 mmol) and Et₃N (0.075 mL, 0.50 mmol). The mixture was stirred at room temperature for 2H, and then concentrated under reduced pressure. The residue was partitioned between CH₂Cl₂ and saturated NaHCO₃. The organic layer was dried over MgSO₄, filtered, and concentrated by rotary evaporation under reduced pressure. The crude material was purified by silica gel column chromatography (EtOAc/hexane=4:1) to yield carbamate 33 (224 mg, 70%). C₄₆H₆₆N₆O₁₅; TLC (EtOAc/hexane=4:1) R_(f)=0.15; white solid, mp 113-115° C.; [α]²⁴ _(D)+13.5 (c=1.0, CHCl₃); IR ν_(max) (neat) 3308, 2979, 2930, 1728, 1641, 1611, 1511, 1253, 1140 cm⁻¹; ¹H NMR (400 MHz, CDCl₃) δ 11.36 (1H, s), 8.45 (1H, d, J=8.4 Hz), 7.44 (1H, d, J=15.6 Hz), 7.32 (1H, t, J=2.0 Hz), 6.98 (2H, d, J=6.8 Hz), 6.79 (1H, d, J=8.8 Hz), 6.50 (1H, d, J=9.2 Hz), 6.31 (1H, d, J=15.6 Hz), 6.05-5.95 (2H, m), 5.84 (1H, d, J=1.2 Hz), 5.34 (1H, d, J=17.2 Hz), 5.26-5.18 (3H, m), 5.12-5.05 (2H, m), 4.56-4.52 (4H, m), 4.30-4.25 (2H, m), 4.22-4.12 (3H, m), 4.08-4.01 (3H, m), 3.98-3.94 (1H, m), 3.50-3.45 (1H, m), 3.38-3.32 (3H, m), 3.05-3.00 (1H, m), 1.97 (3H, s), 1.41 (9H, s), 1.33 (9H, s), 1.30-1.18 (9H, m); ¹³C NMR (100 MHz, CDCl₃) δ 171.0, 166.5, 163.0, 161.2, 156.9, 155.6, 152.6, 149.7, 148.3, 145.1, 139.9, 133.0, 133.0, 128.2, 121.4, 119.2, 117.6, 113.5, 113.1, 109.4, 108.6, 83.5, 79.4, 75.0, 69.6, 69.5, 65.6, 61.4, 60.2, 49.2, 47.8, 38.1, 36.1, 29.5, 28.5, 28.1 (3×), 27.8 (3×), 26.3, 25.2, 23.0, 14.0; ESI-HRMS calcd for C₄₆H₆₇N₆O₁₅: 943.4664, found: m/z 943.4662 [M+H]⁺.

ZA-7-CA-Amide Conjugate 7

To a solution of compound 33 (100 mg, 0.11 mmol) in THF (4 mL) was added 1 M KOH (2 mL). The solution was stirred at room temperature for 1.5 h, neutralized by Dowex 50W×8 (H⁺), filtered and concentrated under reduced pressure. The residue was dissolved in anhydrous THF (5 mL), then added Pd(PPh₃)₄ (23 mg, 0.02 mmol) and morpholine (0.4 mL, 4.5 mmol). The mixture was stirred at room temperature for 4 h. The resulting solution was extracted with 1 M HCl and brine, dried over MgSO₄, concentrated under reduced pressure, and purified by flash silica gel column chromatography (MeOH/CH₂Cl₂=1:9 to MeOH/CH₂Cl₂=1:4). The residue was dissolved in CH₂Cl₂ (2 mL) and TFA (2 mL). After stirring at room temperature for 3H, the mixture was evaporated under reduced pressure. The residue was triturated with Et₂O, and centrifuged to give ZA-7-CA-amide conjugate 7 (35 mg, 56%). C₂₅H₃₄N₆O₁₁; yellow solid, mp 288-290° C.; [α]¹⁹ _(D)+220.1 (c=0.50, H₂O); IR ν_(max) (neat) 3422, 1702, 1655, 1545, 1259, 1202, 1131, 1064 cm⁻¹; ¹H NMR (400 MHz, D₂O) δ 7.36 (1H, d, J=15.6 Hz), 7.13 (1H, s), 7.06 (1H, d, J=7.6 Hz), 6.94 (1H, d, J=8.0 Hz), 6.40 (1H, dd, J=16.0 Hz), 5.74 (1H, d, J=2.0 Hz), 4.99 (1H, dd, J=9.2, 2.0 Hz), 4.46 (1H, dd, J=8.4, 3.2 Hz), 4.45 (1H, d, J=7.2 Hz), 4.18-4.11 (2H, m), 3.72 (1H, dd, J=12.0, 2.0 Hz), 3.57-3.52 (1H, m), 3.35 (2H, t, J=6.0 Hz), 3.18 (2H, t, J=6.4 Hz), 2.02 (3H, s), 1.78 (2H, m); ¹³C NMR (100 MHz, D₂O) δ 173.8, 169.0, 168.5, 157.0, 156.7, 149.2, 146.3, 144.2, 140.7, 127.4, 121.9, 117.8, 116.2, 114.8, 104.8, 75.3, 69.8, 68.6, 62.5, 51.8, 47.4, 38.2, 37.0, 28.4, 21.9; ESI-HRMS (negative mode) calcd for C₂₅H₃₃N₆O₁₁: 593.2207, found: m/z 593.2222 [M−H]⁻.

Example 21 Synthesis of ZA-7-HNAP-Amide Conjugate 8 (FIG. 11) N-3-(Tert-butoxycarbonylamino)propyl 1-hydroxynaphthene-2-carboxamide (34)

To a solution of HNAP (300 mg, 1.60 mmol) in CH₂Cl₂ (5 mL) was added amine 30 (306 mg, 1.76 mmol), EDCI (334 mg, 1.76 mmol) and 4-dimethylaminopyridine (215 mg, 1.76 mmol). The mixture was stirred at room temperature for 12 h. The resulting solution was washed with 1 M HCl, dried over MgSO₄, concentrated under reduced pressure, and purified by flash silica gel column chromatography (hexane to EtOAc/hexane=1:4) to afford the coupling product 34 (378 mg, 69%). C₁₉H₂₄N₂O₄; TLC (EtOAc/hexane=3:7) R=0.31; red foam; IR ν_(max) (neat) 3356, 2976, 1692, 1619, 1609, 1538, 1392, 1365, 1282 cm⁻¹; ¹H NMR (400 MHz, CDCl₃) δ 14.16 (1H, s), 8.43 (1H, d, J=8.4 Hz), 7.94 (1H, s), 7.73 (1H, d, J=8.0 Hz), 7.53 (3H, m), 7.25 (1H, d, J=8.8 Hz), 5.22 (1H, s), 3.51 (2H, m), 3.23 (2H, t, J=6.0 Hz), 1.71 (2H, t, J=5.2 Hz), 1.49 (9H, s); ¹³C NMR (100 MHz, CDCl₃) δ 170.7, 160.2, 157.1, 136.0, 128.5, 127.1, 125.4, 123.5, 121.3, 118.0, 106.8, 79.5, 77.0, 36.9, 35.5, 29.8, 28.2 (3×); ESI-HRMS (negative mode) calcd for C₁₉H₂₃N₂O₄: 343.1658, found: m/z 343.1648 [M−H]⁻.

Compound 36

To a solution of N-Boc compound 34 (189 mg, 0.55 mmol) in CH₂Cl₂ (2 mL) was added TFA (2 mL). After stirring at room temperature for 1 h, the mixture was evaporated under reduced pressure to give the corresponding amine 35. The crude amine product was dissolved in CH₃CN (7 mL), and added carbonate 15 (236 mL, 0.31 mmol) and Et₃N (0.05 mL, 0.34 mmol). The mixture was stirred at room temperature for 2H, and then concentrated under reduced pressure. The residue was partitioned between CH₂Cl₂ and saturated NaHCO₃. The organic layer was dried over MgSO₄, filtered, and concentrated by rotary evaporation under reduced pressure. The crude material was purified by silica gel column chromatography (EtOAc/hexane=1:4 to EtOAc/hexane=2:3) to yield carbamate 36 (165 mg, 61%). C₄₂H₅₈N₆O₁₄; TLC (EtOAc/hexane=1:1) R_(f)=0.55; white solid, mp 157-160° C.; [α]²⁴ _(D)+14.6 (c=0.5, CHCl₃); IR ν_(max) (neat) 3413, 1641, 1634, 1252, 1142 cm⁻¹; ¹H NMR (400 MHz, CDCl₃) δ 14.08 (1H, s), 11.36 (1H, s), 8.56 (1H, d, J=8.4 Hz), 8.46 (1H, s), 8.35 (1H, d, J=8.0 Hz), 7.66 (1H, t, J=8.0 Hz), 7.60 (1H, t, J=8.8 Hz), 7.50-7.41 (2H, m), 7.16 (1H, d, J=8.8 Hz), 6.62 (1H, d, J=8.4 Hz), 5.88 (1H, d, J=2.0 Hz), 5.30 (1H, d, J=5.2 Hz), 5.13-5.08 (2H, m), 4.35-4.15 (6H, m), 4.11-3.95 (3H, m), 3.71-3.66 (1H, m), 3.41 (2H, br), 3.04 (1H, br), 1.92 (3H, s), 1.46 (9H, s), 1.43 (9H, s), 1.34-1.25 (9H, m); ¹³C NMR (100 MHz, CDCl₃) δ 171.4, 171.0, 162.5, 161.2, 160.3, 156.9, 155.6, 152.6, 145.3, 136.1, 128.3, 127.1, 125.5, 125.3, 123.6, 122.0, 117.7, 109.2, 108.8, 107.2, 83.8, 79.9, 77.3, 74.9, 69.6, 65.7, 61.5, 49.4, 47.5, 38.0, 35.9, 28.3, 28.1 (3×), 27.6 (3×), 26.3, 25.2, 23.0, 14.0; ESI-HRMS (negative mode) alcd for C₄₂H₅₇N₆O₁₄: 869.3933, found: m/z 869.3932 [M−H]⁻.

ZA-7-HNAP-Amide Conjugate 8

To a solution of compound 36 (95 mg, 0.11 mmol) in THF (3 mL) was added 1 M KOH (1 mL). The solution was stirred at room temperature for 1.5 h, neutralized by Dowex 50W×8 (H⁺), filtered and concentrated under reduced pressure. The residue was dissolved in CH₂Cl₂ (2 mL) and treated with TFA (2 mL). After stirring at room temperature for 3H, the mixture was evaporated under reduced pressure. The residue was triturated with Et₂O, and centrifuged to give ZA-7-HNAP-amide conjugate 8 (41 mg, 62%). C₂₇H₃₄N₆O₁₀; light yellow solid, mp 230-232° C.; [α]²⁴ _(D)+17.1 (c=1.0, CH₃OH); IR ν_(max) (neat) 3423, 1637, 1405, 1261, 1145 cm⁻¹; ¹H NMR (400 MHz, CD₃OD) δ 8.33 (1H, d, J=8.0 Hz), 7.78 (1H, d, J=8.0 Hz), 7.72 (1H, d, J=8.8 Hz), 7.57 (1H, t, J=9.2 Hz), 7.49 (1H, t, J=8.0 Hz), 7.30 (1H, d, J=8.8 Hz), 5.55 (1H, d, J=2.4 Hz), 4.93 (1H, dd, J=9.2, 6.4 Hz), 4.46 (1H, dd, J=10.4, 6.0 Hz), 4.32 (1H, dd, J=9.2, 2.0 Hz), 4.22 (1H, d, J=10.0 Hz), 4.07-4.02 (1H, m), 3.66-3.61 (1H, m), 3.60-3.43 (3H, m), 3.27-3.20 (1H, m), 3.17-3.10 (1H, m), 1.97 (3H, s), 1.89-1.75 (2H, m); ¹³C NMR (100 MHz, CD₃OD) δ 172.6, 171.5, 169.2, 160.2, 157.9, 157.2, 150.9, 136.7, 128.7, 127.4, 125.7, 125.6, 123.4, 122.2, 118.1 (2×), 107.5, 103.0, 76.0, 70.2, 69.2, 63.6, 52.6, 38.2, 36.6, 29.3, 21.9; ESI-HRMS calcd for C₂₇H₃₅N₆O₁₀: 603.2415, found: m/z 603.2405 [M+H]⁺.

Example 22 Synthesis of ZA-1-CA Conjugate 9 (FIG. 12) 5-Acetylamino-4-[2,3-bis(tert-butoxycarbonyl)guanidino]-6-[2,2-dimethyl-[1,3]dioxolan-4-yl)-hydroxymethyl]-5,6-dihydro-4H-pyran-2-carboxylic acid (37)

To a solution of ester 14 (500 mg, 0.83 mmol) in THF (10 mL) was added 1 M KOH (5 mL). The solution was stirred at room temperature for 1.5 h, neutralized by Dowex 50W×8 (H⁺), filtered and concentrated under reduced pressure. The residue was dissolved in CH₂Cl₂ (20 mL) and extracted with 1 M HCl and brine, dried over MgSO₄, concentrated under reduced pressure, and purified by flash silica gel column chromatography (MeOH/CH₂Cl₂=1:9) to afford acid 37 (428 mg, 90%). C₂₅H₄₀N₄O₁₁; white solid, mp 118-120° C.; ¹H NMR (400 MHz, CDCl₃) δ 11.28 (1H, s), 8.57 (1H, d, J=7.6 Hz), 8.00 (1H, d, J=4.4 Hz), 5.74 (1H, d, J=2.4 Hz), 5.08-5.03 (1H, m), 4.32 (1H, d, J=5.6 Hz), 4.09-3.89 (4H, m), 3.41 (1H, d, J=8.8 Hz), 1.95 (3H, s), 1.41 (9H, s), 1.39 (9H, s), 1.33 (3H, s), 1.26 (3H, s); ¹³C NMR (100 MHz, CDCl₃) 174.1, 171.2, 162.0, 157.3, 152.3, 146.7, 109.2, 106.9, 84.1, 79.9, 77.9, 73.6, 69.6, 67.2, 51.6, 48.3, 28.0 (3×), 27.8 (3×), 26.9, 24.9, 22.7; ESI-HRMS (negative mode) calcd for C₂₅H₃₉N₄O₁₁: 571.2615, found: m/z 571.2615 [M−H]⁻.

5-Acetylamino-4-[2,3-bis(tert-butoxycarbonyl)guanidino]-6-[(2,2-dimethyl-[1,3]dioxolan-4-yl)-hydroxymethyl]-5,6-dihydro-4H-pyran-2-carboxylic acid 3-hydroxypropyl ester (38)

To a solution of acid 37 (402 mg, 0.80 mmol) in MeOH (5 mL) was added KOH (45 mg, 0.80 mmol). The mixture was stirred at room temperature for 30 min, and then concentrated under reduced pressure to give the corresponding potassium salt. The salt was dissolved in DMF (5 mL), and then added 3-iodo-1-propanol (93 μL, 1.00 mmol). After stirring at 50° C. for 4 h, the mixture was evaporated under reduced pressure. The residue was dissolved in CH₂Cl₂ (20 mL) and extracted with 1M HCl and brine, dried over MgSO₄, concentrated under reduced pressure, and purified by flash silica gel column chromatography (EtOAc/hexane=1:1 to EtOAc/hexane=4:1) to afford ester 38 (285 mg, 57%). C₂₈H₄₆N₄O₁₂; colorless solid, mp 86-88° C.; TLC (EtOAc/hexane=4:1) R_(f)=0.48; [α]²¹ _(D) −23.1 (c=1.0, CHCl₃); IR ν_(max) (neat) 3275, 2931, 1727, 1646, 1611, 1558, 1150 cm⁻¹; ¹H NMR (400 MHz, CDCl₃) δ 11.32 (1H, s), 8.58 (1H, d, J=7.6 Hz), 7.96 (1H, d, J=0.8 Hz), 5.76 (1H, d, J=2.0 Hz), 5.26 (1H, d, J=4.4 Hz), 5.12-5.08 (1H, m), 4.34-4.28 (2H, m), 4.14-4.10 (1H, m), 4.07-3.91 (3H, m), 3.68 (2H, t, J=6.0 Hz), 3.47-3.44 (1H, m), 1.96 (3H, s), 1.90-1.87 (2H, m), 1.46 (9H, s), 1.44 (9H, s), 1.37 (3H, s), 1.31 (3H, s); ¹³C NMR (100 MHz, CDCl₃) δ 173.9, 162.2, 161.6, 157.5, 152.6, 146.7, 109.1, 107.0, 84.3, 80.0, 78.5, 74.0, 69.7, 67.4, 62.7, 59.1, 51.7, 48.3, 31.4, 28.1 (3×), 27.9 (3×), 26.9, 25.1, 22.9; ESI-HRMS calcd for C₂₈H₄₇N₄O₁₂: 631.3190, found: m/z 631.3187 [M+H]⁺.

Compound 39

To a solution of 38 (86 mg, 0.14 mmol) in CH₂Cl₂ (5 mL) was added the allyl ether of caffeic acid 18 (39 mg, 0.15 mmol), EDCI (29 mg, 0.15 mmol) and 4-dimethylaminopyridine (18 mg, 0.15 mmol). The mixture was stirred at room temperature for 1.5 h. The resulting solution was extracted with 1 M HCl, saturated NaHCO₃ and brine, dried over MgSO₄, concentrated under reduced pressure, and purified by flash silica gel column chromatography (EtOAc/hexane=1:4 to EtOAc/hexane=2:3) to afford compound 39 (101 mg, 85%). C₄₃H₆₀N₄O₁₅; light yellow solid, mp 70-72° C.; TLC (EtOAc/hexane=2:3) R_(f)=0.24; [α]²⁰ _(D)−18.7 (c=1.0, CHCl₃); IR ν_(max) (neat) 3311, 2979, 2930, 1724, 1646, 1607, 1253, 1153 cm⁻¹; ¹H NMR (400 MHz, CDCl₃) δ 11.34 (1H, s), 8.60 (1H, d, J=8.0 Hz), 7.96 (1H, d, J=5.2 Hz), 7.56 (1H, d, J=16.0 Hz), 7.03 (2H, s), 6.83 (1H, d, J=8.8 Hz), 6.22 (1H, d, J=16.0 Hz), 6.21-5.98 (2H, m), 5.77 (1H, d, J=2.0 Hz), 5.39 (2H, dd, J=17.2, 5.2 Hz), 5.28-5.44 (2H, m), 5.14-5.10 (1H, m), 4.61-4.55 (4H, m), 4.37-4.33 (1H, m), 4.30-4.24 (4H, m), 4.16-4.12 (2H, m), 4.06-3.93 (3H, m), 2.09-2.05 (2H, m), 1.98 (3H, s), 1.47 (9H, s), 1.45 (9H, s), 1.38 (3H, s), 1.32 (3H, s); ¹³C NMR (100 MHz, CDCl₃) δ 173.9, 166.9, 162.2, 161.3, 157.5, 152.6, 150.6, 148.5, 146.7, 144.8, 133.0, 132.8, 127.4, 122.7, 117.8, 117.7, 115.4, 113.3, 112.6, 109.0, 106.9, 84.2, 80.0, 78.5, 77.2, 73.9, 69.8, 69.7, 67.4, 62.1, 60.6, 51.8, 48.3, 29.6, 28.1 (3×), 27.9 (3×), 26.9, 25.0, 22.8; ESI-HRMS (negative mode) calcd for C₄₃H₅₉N₄O₁₅: 803.3351, found: m/z 803.3351 [M−H]⁻.

ZA-1-CA Conjugate 9

To a solution of compound 39 (60 mg, 0.07 mmol) in anhydrous THF (4 mL) was added Pd(PPh₃)₄ (12 mg, 0.01 mmol) and morpholine (0.1 mL, 1.4 mmol). The mixture was stirred at room temperature for 4 h. The resulting solution was extracted with 1 M HCl and brine, dried over MgSO₄, concentrated under reduced pressure, and purified by flash silica gel column chromatography (MeOH/CH₂Cl₂=1:20 to MeOH/CH₂Cl₂=1:9). The residue was dissolved in CH₂Cl₂ (2 mL) and TFA (2 mL). After stirring at room temperature for 3 h, the mixture was evaporated under reduced pressure. The residue was triturated with Et₂O, and centrifuged to give ZA-1-CA conjugate 9 (23 mg, 61%). C₂₄H₃₂N₄O₁₁; orange solid, mp 135-137° C.; [α]²⁰ _(D)+6.6 (c=1.0, H₂O); IR ν_(max) (neat) 3414, 1675, 1636, 1276, 1203, 1185, 1141 cm⁻¹; ¹H NMR (400 MHz, CD₃OD) δ 7.55 (1H, d, J=16.0 Hz), 7.04 (1H, d, J=2.0 Hz), 6.95 (1H, dd, J=8.0, 2.0 Hz), 6.79 (1H, d, J=8.0 Hz), 6.25 (1H, d, J=15.6 Hz), 5.90 (1H, d, J=2.4 Hz), 4.35-4.19 (6H, m), 3.88-3.81 (2H, m), 3.71-3.67 (3H, m), 2.11-2.07 (2H, m), 1.99 (3H, s); ¹³C NMR (100 MHz, CD₃OD) δ 174.6, 169.3, 163.4, 159.0, 149.8, 147.3, 147.0, 146.6, 127.8, 123.2, 116.7, 115.3, 115.0, 109.0, 78.1, 71.5, 70.3, 64.8, 63.5, 62.1, 51.5, 50.0, 29.2, 22.8; ESI-HRMS calcd for C₂₄H₃₃N₄O₁₁: 553.2146, found: m/z 553.2150 [M+H]⁺.

Example 23 Synthesis of ZA-1-MCA Conjugate 10 (FIG. 13) Compound 40

To a solution of alcohol 38 (40 mg, 0.06 mmol) in CH₂Cl₂ (3 mL) was added (3,4-methylenedioxy)cinnamic acid (MCA, 13 mg, 0.07 mmol), EDCI (13 mg, 0.07 mmol) and 4-dimethylaminopyridine (9 mg, 0.07 mmol). The mixture was stirred at room temperature for 1.5 h. The resulting solution was extracted with 1 M HCl, saturated NaHCO₃ and brine, dried over MgSO₄, concentrated under reduced pressure, and purified by flash silica gel column chromatography (EtOAc/hexane=1:4 to EtOAc/hexane=2:3) to afford compound 40 (43 mg, 84%). C₃₈H₅₂N₄O₁₅; pale yellow solid, mp 73-75° C.; TLC (EtOAc/hexane=1:1) R_(f)=0.20; [α]²⁰ _(D) −17.3 (c=1.0, CHCl₃); IR ν_(max) (neat) 2924, 1723, 1646, 1607, 1250, 1154, 1127 cm⁻¹; ¹H NMR (400 MHz, CDCl₃) δ 11.34 (1H, s), 8.59 (1H, d, J=7.9 Hz), 7.95 (1H, d, J=5.2 Hz), 7.55 (1H, d, J=16.0 Hz), 7.00 (1H, s), 6.98 (1H, d, J=8.0 Hz), 6.78 (1H, d, J=8.0 Hz), 6.22 (1H, d, J=15.6 Hz), 5.97 (2H, s), 5.77 (1H, d, J=1.6 Hz), 5.27 (1H, br), 5.13-5.09 (1H, m), 4.38-4.32 (1H, m), 4.29-4.25 (3H, m), 4.21-4.13 (2H, m), 4.06-3.90 (3H, m), 2.09-2.05 (2H, m), 1.98 (3H, s), 1.48 (9H, s), 1.46 (9H, s), 1.39 (3H, s), 1.33 (3H, s); ¹³C NMR (100 MHz, CDCl₃) δ 174.0, 166.9, 162.3, 161.4, 157.6, 152.6, 149.6, 148.3, 146.8, 144.7, 128.7, 124.4, 115.7, 109.1, 108.5, 106.9, 106.5, 101.5, 84.3, 80.1, 78.6, 74.0, 69.8, 67.5, 62.2, 60.8, 51.9, 48.4, 29.7, 28.2 (3×), 28.0 (3×), 27.0, 25.1, 22.9; ESI-HRMS (negative mode) calcd for C₃₈H₅₁N₄O₁₅: 803.3351, found: m/z 803.3351 [M−H]⁻.

ZA-1-MCA Conjugate 10

To a solution of compound 40 (48 mg, 0.06 mmol) in CH₂Cl₂ (2 mL) was added TFA (2 mL). After stirring at room temperature for 3H, the mixture was evaporated under reduced pressure. The residue was triturated with Et₂O, and centrifuged to give ZA-1-MCA conjugate 10 (21 mg, 62%). C₂₅H₃₂N₄O₁₁; white solid, mp 130-132° C.; [α]¹⁹ _(D)+17.2 (c=0.5, CH₃OH); IR ν_(max) (neat) 3395, 1682, 1634, 1253, 1203, 1040 cm⁻¹; ¹H NMR (400 MHz, CD₃OD) δ 7.61 (1H, d, J=16.0 Hz), 7.17 (1H, d, J=1.2 Hz), 7.09 (1H, dd, J=8.0, 2.0 Hz), 6.85 (1H, d, J=8.0 Hz), 6.37 (1H, d, J=16.0 Hz), 6.00 (2H, s), 5.89 (1H, d, J=2.8 Hz), 4.47-4.19 (7H, m), 3.90-3.81 (2H, m), 3.71-3.67 (2H, m), 2.11 (2H, t, J=6.4 Hz), 2.01 (3H, s); ¹³C NMR (100 MHz, CD₃OD) δ 173.6, 168.1, 162.5, 158.1, 150.6, 149.2, 145.7, 145.6, 131.5, 129.3, 125.1, 115.6, 108.7, 107.9, 106.7, 102.3, 77.2, 70.5, 69.5, 63.9, 62.5, 61.2, 50.6, 28.3, 21.8; ESI-HRMS calcd for C₂₅H₃₃N₄O₁₁: 565.2146, found: m/z 565.2144 [M+H]⁺.

Example 24 Synthesis of ZA-1-ME Conjugate 11 (FIG. 14) Compound 41

To a solution of 38 (100 mg, 0.16 mmol) in CH₂Cl₂ (5 mL) was added the allyl ether of mesalazine 22 (47 mg, 0.16 mmol), EDCI (31 mg, 0.16 mmol) and 4-dimethylaminopyridine (18 mg, 0.16 mmol). The mixture was stirred at room temperature for 1.5 h. The resulting solution was extracted with 1M HCl, saturated NaHCO₃ and brine, dried over MgSO₄, concentrated under reduced pressure, and purified by flash silica gel column chromatography (EtOAc/hexane=1:4 to EtOAc/hexane=2:3) to afford ester 41 (98 mg, 70%). C₄₃H₆₃N₅O₁₆; light yellow solid, mp 82-84° C.; TLC (EtOAc/hexane=1:1) R=0.18; [α]²⁰ _(D) −21.0 (c=1.0, CHCl₃); IR ν_(max) (neat) 3312, 2978, 2927, 1725, 1646, 1248, 1231, 1155 cm⁻¹; ¹H NMR (400 MHz, CDCl₃) δ 11.32 (1H, s), 8.55 (1H, d, J=8.0 Hz), 7.90 (1H, d, J=3.6 Hz), 7.61 (1H, s), 7.58 (1H, d, J=2.8 Hz), 6.87 (1H, d, J=9.2 Hz), 6.69 (1H, s), 6.07-5.95 (1H, m), 5.73 (1H, d, J=2.4 Hz), 5.41 (2H, dd, J=16.8, 1.0 Hz), 5.25-5.21 (2H, m), 5.10-5.01 (1H, m), 4.54 (2H, d, J=5.2 Hz), 4.38-4.29 (4H, m), 4.16-3.91 (2H, m), 3.48-3.44 (2H, m), 2.14-2.07 (2H, m), 1.98 (3H, s), 1.47 (9H, s), 1.46 (18H, s), 1.39 (3H, s), 1.32 (3H, s); ¹³C NMR (100 MHz, CDCl₃) 173.9, 165.7, 162.3, 161.3, 157.5, 154.1, 153.2, 152.6, 146.5, 132.9, 131.4, 131.0, 120.6, 117.7, 114.6, 114.0, 109.1, 107.1, 84.3, 80.1, 78.5, 77.2, 74.0, 71.2, 70.1, 69.8, 67.4, 62.4, 61.5, 51.5, 48.3, 36.6, 29.6, 28.3 (3×), 28.2 (3×), 28.0 (3×), 27.0, 25.1, 22.9; ESI-HRMS (negative mode) calcd for C₄₃H₆₂N₅O₁₆: 904.4192, found: m/z 904.4214 [M−H]⁻.

ZA-1-ME Conjugate 11

To a solution of compound 41 (34 mg, 0.04 mmol) in anhydrous THF (3 mL) was added Pd(PPh₃)₄ (12 mg, 0.01 mmol) and morpholine (0.1 mL, 1.4 mmol). The mixture was stirred at room temperature for 4 h. The resulting solution was extracted with 1M HCl and brine, dried over MgSO₄, concentrated under reduced pressure, and purified by flash silica gel column chromatography (MeOH/CH₂Cl₂=1:20 to MeOH/CH₂Cl₂=1:9). The residue was dissolved in CH₂Cl₂ (2 mL) and TFA (2 mL). After stirring at room temperature for 3H, the mixture was evaporated under reduced pressure. The residue was triturated with Et₂O, and centrifuged to give ZA-1-ME conjugate 11 (23 mg, 62%). C₂₂H₃₁N₅O₁₀; yellow solid, mp 126-128° C.; [α]¹⁸ _(D)+18.6 (c=0.25, CH₃OH); IR ν_(max) (neat) 3422, 1677, 1493, 1204, 1137 cm⁻¹; ¹H NMR (400 MHz, CD₃OD) δ 7.73 (1H, d, J=2.8 Hz), 7.38 (1H, dd, J=9.2, 2.8 Hz), 5.86 (1H, d, J=2.8 Hz), 4.53-4.38 (6H, m), 4.20-4.16 (1H, m), 3.89-3.80 (2H, m), 3.71-3.66 (3H, m), 2.21 (2H, t, J=6.4 Hz), 2.00 (3H, s); ¹³C NMR (100 MHz, CD₃OD) δ 173.7, 172.1, 169.5, 162.4, 160.0, 158.1, 145.7, 129.1, 122.2, 119.3, 113.4, 108.1, 77.3, 70.6, 69.4, 64.1, 63.9, 62.8, 62.5, 50.6, 28.1, 21.8; ESI-HRMS calcd for C₂₂H₃₂N₅O₁₀: 526.2149, found: m/z 526.2146 [M+H]⁺.

Example 25 Synthesis of ZA-1-HNAP Conjugate 12 (FIG. 15) Compound 42

To a solution of alcohol 38 (117 mg, 0.19 mmol) in CH₂Cl₂ (3 mL) was added the allyl ether of 1-hydroxy-2-naphthoic acid 25 (43 mg, 0.19 mmol), EDCI (36 mg, 0.19 mmol) and 4-dimethylaminopyridine (21 mg, 0.19 mmol). The mixture was stirred at room temperature for 1.5 h. The resulting solution was extracted with 1 M HCl, saturated NaHCO₃ and brine, dried over MgSO₄, concentrated under reduced pressure, and purified by flash silica gel column chromatography (EtOAc/hexane=1:4 to EtOAc/hexane=2:3) to afford compound 42 (105 mg, 68%). C₄₂H₅₆N₄O₁₄; light yellow foam; TLC (EtOAc/hexane=2:3) R_(f)=0.22; [α]¹⁹ _(D) −25.5 (c=1.0, CHCl₃); IR ν_(max) (neat) 2980, 2930, 1726, 1647, 1607, 1565, 1369, 1250, 1152, 1129 cm⁻¹; ¹H NMR (400 MHz, CDCl₃) δ 11.32 (1H, s), 8.57 (1H, d, J=7.6 Hz), 8.22 (1H, d, J=8.0 Hz), 7.94 (1H, d, J=4.8 Hz), 7.81 (1H, d, J=8.8 Hz), 7.58-7.47 (3H, m), 6.21-6.11 (1H, m), 5.75 (1H, d, J=2.0 Hz), 5.45 (1H, dd, J=17.2, 1.2 Hz), 5.27 (2H, d, J=11.6 Hz), 5.09-5.04 (1H, m), 4.61 (1H, d, J=5.6 Hz), 4.45-4.42 (2H, m), 4.38-4.33 (3H, m), 4.16-4.01 (3H, m), 3.97-3.91 (2H, m), 3.47-3.44 (1H, m), 2.18-2.14 (2H, m), 1.97 (3H, s), 1.46 (9H, s), 1.45 (9H, s), 1.37 (3H, s), 1.31 (3H, s); ¹³C NMR (100 MHz, CDCl₃) δ 173.9, 165.9, 162.2, 161.2, 157.5, 156.9, 152.6, 146.7, 136.6, 133.6, 128.7, 128.3, 127.8, 126.5, 126.4, 123.6, 119.5, 117.6, 109.0, 106.8, 84.2, 80.0, 78.5, 76.5, 73.9, 69.7, 67.4, 62.3, 61.5, 60.3, 51.9, 48.3, 28.1 (3×), 27.9 (3×), 26.9, 25.0, 22.9, 14.1; ESI-HRMS calcd for C₄₂H₅₇N₄O₁₄: 841.3871, found: m/z 841.3907 [M+H]⁺.

ZA-1-HNAP Conjugate 12

To a solution of compound 42 (65 mg, 0.08 mmol) in anhydrous THF (4 mL) was added Pd(PPh₃)₄ (12 mg, 0.01 mmol) and morpholine (0.1 mL, 1.4 mmol). The mixture was stirred at room temperature for 4 h. The resulting solution was extracted with 1 M HCl and brine, dried over MgSO₄, concentrated under reduced pressure, and purified by flash silica gel column chromatography (EtOAc/hexane=1:4 to EtOAc/hexane=1:1). The residue was dissolved in CH₂Cl₂ (2 mL) and TFA (2 mL). After stirring at room temperature for 3H, the mixture was evaporated under reduced pressure. The residue was triturated with Et₂O, and centrifuged to give ZA-1-HNAP conjugate 12 (33 mg, 75%). C₂₆H₃₂N₄O₁₀; pale yellow solid, mp 120-122° C.; [α]²¹ _(D)+19.3 (c=1.0, MeOH); UV-vis (MeOH) λ_(max) 316 nm (ε=5340), 260 nm (ε=28990); UV-vis (PBS) λ_(max) 316 nm (s=4180), 260 nm (ε=4420); IR ν_(max) (neat) 3422, 1719, 1663, 1638, 1253, 1203, 1139, 1090 cm⁻¹; ¹H NMR (400 MHz, CD₃OD) δ 8.35 (1H, d, J=8.4 Hz), 7.81 (1H, d, J=8.4 Hz), 7.77 (1H, d, J=8.8 Hz), 7.63 (1H, m), 7.53 (1H, m), 7.33 (1H, d, J=8.8 Hz), 5.89 (1H, d, J=2.8 Hz), 4.54-4.51 (2H, m), 4.46-4.41 (4H, m), 4.23-4.18 (1H, m), 3.89-3.81 (2H, m), 3.72-3.67 (2H, m), 2.26-2.20 (2H, m), 2.00 (3H, s); ¹³C NMR (100 MHz, CD₃OD) δ 173.5, 171.3, 162.4, 161.0, 158.0, 145.6, 137.8, 129.8, 127.8, 126.1, 125.0, 124.3, 123.6, 119.0, 108.0, 105.7, 77.1, 70.5, 69.3, 63.8, 62.5, 62.3, 50.4, 47.9, 28.1, 21.7; ESI-HRMS calcd for C₂₆H₃₃N₄O₁₀: 561.2197, found: m/z 561.2194 [M+H]⁺.

Example 26 Synthesis of ZA-7-Nap Conjugate 43 (FIG. 19) 5-Acetylamino-4-[2,3-bis(tert-butoxycarbonyl)guanidino]-6-[(2,2-dimethyl-[1,3]dioxolan-4-yl)-(4-nitrophenoxy)carbonyloxy)methyl]-5,6-dihydro-4H-pyran-2-carboxylic acid ethyl ester (15a)

Methyl ester 13a, a counterpart of the ethyl ester 13, was prepared according to the reported procedure (Chandler, M. et al. J. Chem. Soc., Perkin Trans. 11995, 1173). Saponification of 13a with NaOMe in methanol, followed by acid-catalyzed acetalization with 2,2-dimethoxypropane afforded compound 14a, which was treated with 4-nitrophenyl chloroformate and 4-dimethylaminopyridine in anhydrous pyridine to give carbonate 15a in 36% overall yield.

Carbamate Compound 53

To a solution of naproxen (100 mg, 0.43 mmol) in CH₂Cl₂ (2 mL) was added EDCI (92 mg, 0.48 mmol) and DMAP (63 mg, 0.52 mmol). After 5 min, 3-(tert-butoxycarbonyl)amino-1-propanol (91 mg, 0.52 mmol) was added. The mixture was stirred at room temperature for 1.5 h. The mixture was extracted with 1 M HCl, saturated NaHCO₃ and brine, dried over MgSO₄, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (EtOAc/hexane, 1:2) to afford an ester product (93 mg, 55%). C₂₂H₂₉NO₅; TLC (EtOAc/hexane, 1:2) R_(f)=0.38; ¹H NMR (400 MHz, CDCl₃) δ 7.68 (2H, d, J=8.1 Hz), 7.64 (1H, s), 7.38 (1H, dd, J=8.6, 1.6 Hz), 7.12 (1H, dd, J=8.5, 2.1 Hz), 7.09 (2H, d, J=2.1 Hz), 4.62 (1H, br, NH), 4.13-4.09 (2H, m), 3.89 (3H, s), 3.83 (1H, q, J=7.32 Hz), 3.04 (2H, m), 1.76-1.69 (2H, m), 1.56 (3H, d, J=7.1 Hz), 1.40 (9H, s); ¹³C NMR (100 MHz, CDCl₃) δ 174.7, 157.6, 155.8, 135.6, 133.7, 129.2, 128.9, 127.1, 126.1, 125.8, 119.0, 105.6, 79.1, 62.2, 55.2, 45.4, 37.2, 29.0, 28.3, 18.4; HRMS calcd for C₂₂H₃₀NO₅: 388.2124, found: m/z 388.2125 [M+H]⁺.

The above-prepared product (69 mg, 0.18 mmol) was treated with trifluoroacetic acid (1.5 mL) in CH₂Cl₂ (4 mL) for 15 min, and then concentrated under reduced pressure to give naproxen 3-aminopropyl ester. This compound was dissolved in CH₃CN (7 mL), and 15a (112 mg, 0.15 mmol) and Et₃N (0.080 mL, 0.59 mmol) were added. The mixture was stirred at room temperature for 12 h, and then concentrated under reduced pressure. The residue was purified by silica gel column chromatography (EtOAc/hexane, 1:2 to 2:1) to afford the carbamate product 53 (119 mg, 90%). C₄₄H₆₁N₅O₁₅; yellow oil; TLC (EtOAc:Hexane=2:1) R_(f)=0.38; ¹H NMR (400 MHz, CDCl₃) δ 11.36 (1H, s), 8.41 (1H, d, J=8.4 Hz), 7.68 (2H, dd, J=8.7, 3.4 Hz), 7.63 (1H, s), 7.36 (1H, d, J=8.7 Hz), 7.11-7.09 (2H, m), 6.11 (1H, d, J=9.4 Hz), 5.85 (1H, d, J=1.6 Hz), 5.21-5.14 (2H, m), 4.91 (1H, t, J=6.4 Hz), 4.34-4.30 (2H, m), 4.14-4.08 (3H, m), 4.06-4.02 (1H, m), 3.97-3.94 (1H, m), 3.88 (3H, s), 3.82 (1H, q, J=7.0 Hz), 3.75 (3H, s), 3.13-3.01 (2H, m), 1.84 (3H, s), 1.79-1.71 (2H, m), 1.53 (3H, d, J=7.1 Hz), 1.45 (18H, s), 1.33 (3H, s), 1.32 (3H, s); ¹³C NMR (100 MHz, CDCl₃) δ 174.7, 170.7, 162.9, 161.9, 157.6, 157.0, 155.5, 152.7, 145.3, 135.6, 133.6, 129.3, 128.9, 127.2, 126.1, 125.9, 118.9, 109.8, 108.9, 105.6, 83.7, 79.6, 77.5, 74.6, 69.7, 65.9, 62.2, 55.3, 52.3, 48.8, 48.3, 45.4, 37.9, 28.7, 28.2, 28.0, 26.5, 25.4, 23.0, 18.4; HRMS calcd for C₄₄H₆₂N₅O₁₅: 900.4242, found: m/z 900.4245 [M+H]⁺.

ZA-7-Nap 43

A solution of compound 53 (101 mg, 0.112 mmol) in THF/MeOH (1:1, 6 mL) and 1 M aqueous K₂CO₃ (6 mL) was stirred at room temperature for 7 h. The mixture was neutralized by Dowex 50W×8, filtered and concentrated under reduced pressure. The residue was dissolved in CH₂Cl₂ (2.5 mL), and TFA (0.5 mL) was added. After stirring at room temperature for 30 min, the mixture was concentrated under reduced pressure. The residue was purified by RP-18 reversed-phase column chromatography (MeOH/H₂O, 20:80 to 80:20) and subjected to lyophilization to afford ZA-7-Nap 43 (72 mg, 72%). The purity of product was 96% as shown by HPLC on an HC-C18 column (Agilent, 4.6×250 mm, 5 μm), t_(R)=18.5 min (gradients of MeOH/H₂O, 40% to 100% in 30 min, 1 mL/min). C₃₀H₃₉N₅O₁₁; White solid; ¹H NMR (400 MHz, CD₃OD) δ 7.74-7.71 (2H, m), 7.66 (1H, s), 7.39 (1H, dd, J=8.6, 1.5 Hz), 7.20 (1H, d, J=2.3 Hz), 7.11 (1H, dd, J=9.0, 2.5 Hz), 5.88 (1H, d, J=2.5 Hz), 4.97 (1H, dd, J=9.3, 2.5 Hz), 4.55 (1H, dd, J=9.9, 2.2 Hz), 4.39 (1H, dd, J=8.8, 2.2 Hz), 4.19-4.10 (3H, m), 4.02-3.97 (1H, m), 3.92-3.87 (4H, m), 3.62 (1H, dd, J=11.9, 2.8 Hz), 3.47 (1H, dd, J=11.9, 5.9 Hz), 3.12-3.00 (2H, m), 1.90 (3H, s), 1.79-1.72 (2H, m), 1.54 (3H, d, J=7.1 Hz); ¹³C NMR (100 MHz, D₂O) δ 176.7, 173.7, 166.0, 157.2, 156.6, 146.4, 135.8, 133.6, 129.4, 128.8, 127.4, 126.3, 125.9, 118.7, 108.1, 106.0, 75.8, 69.8, 68.9, 63.1, 62.6, 57.4, 55.1, 51.6, 47.3, 45.2, 37.5, 28.0, 22.0, 17.7; HRMS calcd for C₃₀H₄₀N₅O₁₁: 646.2724, found: m/z 646.2722 [M+H]⁺.

Example 27 Synthesis of ZA-7-Ibu Conjugate 44 (FIG. 20) Carbamate Compound 55

To a solution of (S)-(+)-ibuprofen (102 mg, 0.49 mmol) in CH₂Cl₂ (2.5 mL) was added EDCI (124 mg, 0.65 mmol) and DMAP (79 mg, 0.65 mmol). After 5 min, 3-(tert-butoxycarbonyl)amino-1-propanol (93 mg, 0.53 mmol) was added. The mixture was stirred at room temperature for 1.5 h. The mixture was extracted with 1 M HCl, saturated NaHCO₃ and brine, dried over MgSO₄, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (EtOAc/Hexane=3:1) to afford an ester product (127 mg, 72%). C₂₁H₃₃NO₄; TLC (EtOAc:Hexane=1:3) R_(f)=0.63; ¹H NMR (400 MHz, CDCl₃) δ 7.16 (2H, d, J=8.4 Hz), 7.05 (2H, d, J=8.4 Hz), 4.67 (1H, br), 4.08 (2H, t, J=6.6 Hz), 3.65 (1H, q, J=7.4 Hz), 3.01-3.00 (2H, m), 2.41 (2H, d, J=7.1 Hz), 1.84-1.77 (1H, m), 1.74-1.68 (2H, m), 1.45 (3H, d, J=7.1 Hz), 1.40 (9H, s), 0.86 (6H, d, J=7.1 Hz); ¹³C NMR (100 MHz, CDCl₃) 174.7, 155.8, 140.5, 137.6, 129.3, 127.0, 79.0, 62.1, 45.0, 44.9, 37.2, 30.1, 28.9, 28.3, 22.3, 18.2.

The above-prepared product (123.4 mg, 0.34 mmol) was treated with trifluoroacetic acid (1 mL) in CH₂Cl₂ (4 mL) for 20 min, and then concentrated under reduced pressure to give ibuprofen derivative 54.

ZA-7-Ibu Conjugate 44

Compound 54 was dissolved in CH₃CN (10 mL), and 15a (232 mg, 0.31 mmol) and Et₃N (0.180 mL, 1.34 mmol) were added. The mixture was stirred at room temperature for 12 h, and then concentrated under reduced pressure. The residue was purified by silica gel column chromatography (EtOAc/hexane, 2:3 to 1:1) to afford the carbamate product 55 (160 mg, 60%). C₄₃H₆₅N₅O₁₄; White foam; TLC (EtOAc:hexane, 1:1) R_(f)=0.37; ¹H NMR (400 MHz, CDCl₃) δ 11.34 (1H, s), 8.39 (1H, d, J=9.1 Hz), 7.14 (2H, d, J=7.8 Hz), 7.04 (2H, d, J=9.1 Hz), 6.14 (1H, d, J=9.1 Hz), 5.85 (1H, d, J=2.1 Hz), 5.19-5.13 (2H, m), 4.93 (1H, t, J=5.8 Hz), 4.33-4.28 (2H, m), 4.13-4.04 (4H, m), 3.98-3.94 (1H, m), 3.74 (3H, s), 3.64 (1H, q, J=7.4 Hz), 3.11-2.99 (2H, m), 2.39 (2H, d, J=7.9 Hz), 1.84 (3H, s), 1.82-1.69 (3H, m), 1.43-1.42 (21H, m), 1.32 (3H, s), 1.30 (3H, s), 0.84 (6H, d, J=6.6 Hz); ¹³C NMR (100 MHz, CDCl₃) δ 174.8, 170.6, 162.9, 161.9, 156.9, 155.4, 152.6, 145.2, 140.4, 137.6, 129.3, 127.0, 109.8, 108.8, 83.6, 79.5, 77.5, 74.6, 69.6, 65.9, 62.0, 52.3, 48.8, 48.2, 45.0, 44.9, 37.8, 30.1, 28.6, 28.2, 28.0, 26.5, 25.3, 23.0, 22.3, 18.3; HRMS calcd for C₄₃H₆₆N₅O₁₄: 876.4606, found: m/z 876.4605 [M+H]⁺.

Example 28 Synthesis of ZA-9-Nap Conjugate 45 (FIG. 21)

Compound 13 (2455 mg, 3.58 mmol) was treated with NaOEt (194.5 mg, 2.86 mmol) in anhydrous EtOH (15 mL) at room temperature for 1 h. The mixture was neutralized by Dowex 50W×8 (H⁺), filtered, and concentrated under reduced pressure to afford solids of deacetylation product 56.

Et₃N (17.3 mg, 0.17 mmol) and HBTU (81.2 mg, 0.21 mmol) were added to a solution of naproxen (32 mg, 0.13 mmol) in anhydrous CH₂Cl₂ (7 mL). After 5 min, a solution of the above-prepared compound 56 (96 mg, 0.17 mmol) and Et₃N (17.3 mg, 0.17 mmol) in anhydrous CH₂Cl₂ (3 mL) was added. The mixture was stirred at room temperature for 2H, and then concentrated under reduced pressure. The residue was partitioned between EtOAc and saturated NaHCO_(3(aq)). The organic layer was washed with saturated NaHCO_(3(aq)), dried over MgSO₄, filtered, and concentrated by rotary evaporation under reduced pressure. The crude material was purified by silica gel column chromatography (hexane/EtOAc=1:1) to yield compound 57. C₃₈H₅₂N₄O₁₃; white solid; ¹H NMR (400 MHz, CDCl₃) δ 11.29 (1H, s), 8.55 (1H, d, J=3.8 Hz), 7.98 (1H, d, J=2.8 Hz), 7.70-7.64 (3H, m), 7.39-7.36 (1H, m), 7.13-7.08 (2H, m), 5.72 (1H, d, J=1.2 Hz), 5.26 (1H, d, J=2.2 Hz), 5.08-5.03 (1H, m), 4.50 (1H, d, J=5.8 Hz), 4.27-4.04 (5H, m), 3.93-3.81 (6H, m), 3.47-3.44 (1H, m), 1.92 (3H, s), 1.62-1.57 (3H, m), 1.53-1.47 (18H, m), 1.27-1.20 (3H, m); ¹³C NMR (100 MHz, CDCl₃) δ 175.2, 173.9, 162.3, 161.4, 157.7, 157.5, 152.6, 146.7, 135.6, 133.7, 129.3, 128.9, 127.2, 127.2, 126.0, 125.9, 119.0, 106.7, 105.6, 84.3, 79.9, 78.1, 68.8, 68.6, 67.5, 61.6, 55.3, 51.8, 48.4, 45.4, 29.7, 28.2, 28.0, 22.8, 18.6, 18.3, 16.4, 14.0; ESI-HRMS calcd for C₃₈H₅₃N₄O₁₃: 773.3609, found: m/z 773.3594 [M+H]⁺.

By a procedure similar to that for preparation of ZA-7-Nap 43, a solution of compound 53 in THF was treated with 1 M aqueous K₂CO₃ at room temperature to give ZA-9-Nap 45.

Example 29 Synthesis of ZA-G-Nap Conjugate 46 (FIG. 22) Compound 60

To a solution of S-methylisothiourea hemisulfate salt (1085 mg, 7.80 mmol) and NaHCO₃ (655 mg, 7.80 mmol) in H₂O (15 mL) and THF (15 mL) was added (Boc)₂O (1283 mg, 5.88 mmol) dropwise. The mixture was stirred at room temperature under argon for 14 h, and then concentrated under reduced pressure. The residue was partitioned between EtOAc and H₂O. The organic phase was dried over MgSO₄, filtered, and purified by silica gel chromatography (EtOAc/hexane=1:4) to afford the product of tert-butyl[imino(methylthio)methyl]carbamate (1104 mg, 99%). C₇H₁₄N₂O₂S; white solid; ¹H NMR (CDCl₃, 400 MHz) δ 2.39 (3H, s), 1.45 (9H, s); ¹³C NMR (CDCl₃, 100 MHz) δ 172.3, 160.4, 79.1, 27.5, 12.7; HRMS (ESI) calcd for C₇H₁₅N₂O₂S: 191.0854, found: m/z 191.0848 [M+H]⁺.

To a solution of naproxen (115 mg, 0.50 mmol) in anhydrous DMF (5 mL) was added the above-prepared compound (114 mg, 0.6 mmol), HBTU (247 mg, 0.65 mmol) and diisopropylethylamine (130 μL, 0.75 mmol). The mixture was stirred at room temperature under argon for 14 h, and then concentrated under reduced pressure. The residue was diluted with EtOAc and washed with 1 M HCl_((aq)), saturated NaHCO_(3(aq)), and brine. The organic phase was dried over MgSO₄, filtered, and purified by silica gel chromatography (EtOAc/hexane=1:9) to afford the desired product 60 (169 mg, 84%). C₂₁H₂₆N₂O₄S; colorless oil; ¹H NMR (CDCl₃, 400 MHz) δ 12.31 (0.55 H, s), 12.19 (0.45 H, s), 7.69 (3H, m), 7.38 (1H, br), 7.15-7.07 (2H, m), 4.02-3.90 (1H, br), 3.87 (3H, s), 2.30 (3H, s), 1.62 (3H, d, J=6.8 Hz), 1.37 (9H, br); HRMS (ESI) calcd for C₂₁H₂₇N₂O₄S: 403.1692, found: m/z 403.1700 [M+H]⁺.

Compound 61

To a solution of azide 59 (192 mg, 0.42 mmol) in MeOH (10 mL) was added Lindlar catalyst (96 mg). The mixture was stirred at room temperature under an atmosphere of hydrogen for 14 h. The mixture was filtered through a pad of Celite, and the filtrate was concentrated under reduced pressure. The residue was dissolved in CH₂Cl₂ (5 mL) and compound 60 (169 mg, 0.42 mmol), HgCl₂ (125 mg, 0.46 mmol) and Et₃N (64 μL, 0.46 mmol) were added. The mixture was stirred at room temperature under argon for 6 h, filtered through a pad of Celite, and concentrated under reduced pressure. The residue was diluted with EtOAc and washed with 1 M HCl_((aq)), saturated NaHCO_(3(aq)), and brine. The organic phase was dried over MgSO₄, filtered, and purified by silica gel chromatography (EtOAc/hexane, 1:1 to 7:3) to afford the product 61 (293 mg, 88%). C₃₈H₄₈N₄O₁₄; colorless oil; ¹H NMR (CDCl₃, 400 MHz) δ 12.24 (0.6H, s), 11.97 (0.4H, s), 8.98 (0.6H, d, J=8.4 Hz), 8.39 (0.4H, d, J=7.2 Hz), 7.77-7.62 (3H, m), 7.46 (0.4H, d, J=8.4 Hz), 7.33 (0.6H, d, J=8.4 Hz), 7.18-7.07 (2H, m), 6.12 (0.6H, d, J=8.8 Hz), 5.87-5.78 (0.4H, m), 5.42-5.37 (0.6H, m), 5.30-5.23 (1H, m), 5.23-5.18 (0.4H, m), 5.09-4.97 (1H, m), 4.69-4.59 (1H, m), 4.29-4.05 (3H, m), 3.95-3.82 (3H, m), 3.82-3.66 (4H, m), 2.18-1.97 (9H, m), 1.89-1.78 (3H, m), 1.62-1.52 (3H, m), 1.43-1.28 (9H, m); HRMS (ESI) calcd for C₃₈H₄₉N₄O₁₄: 785.3245, found: m/z 782.3236 [M+H]⁺.

ZA-G-Nap 46

To a solution of compound 61 (33 mg, 0.042 mmol) in CH₂Cl₂ (1 mL) was added TFA (1 mL). The mixture was stirred at room temperature for 1 h, and then concentrated under reduced pressure. The residue was dissolved in THF (2 mL), and 1 M KOH_((aq)) (2 mL) was added. The mixture was stirred at room temperature for 30 min, acidified by addition of TFA, and concentrated under reduced pressure. The residue was purified by reversed-phase chromatography (RP-18; 0-100% MeOH in H₂O) to afford ZA-G-Nap 46 (21 mg, 93%). C₂₆H₃₂N₄O₉; white solid; ¹H NMR (D₂O, 400 MHz) δ 7.69 (3H, m), 7.36 (1H, br), 7.21-7.05 (2H, m), 5.52 (1H, m), 4.56-4.46 (1H, m), 4.40-4.31 (1H, m), 4.31-4.21 (1H, m), 4.02-3.76 (6H, m), 3.68-3.55 (2H, m), 1.87 (3H, s), 1.51 (3H, d, J=5.2 Hz); HRMS (ESI) calcd for C₂₆H₃₃N₄O₉: 545.2248, found: m/z 545.2249 [M+H]⁺.

Example 30 Synthesis of ZA-G-Ibu Conjugate 47 (FIG. 23) Compound 62

To a solution of (S)-ibuprofen (103 mg, 0.50 mmol) in anhydrous DMF (5 mL) was added HBTU (247 mg, 0.65 mmol), DIEA (130 μL, 0.75 mmol), and compound tert-butyl[imino(methylthio)methyl]carbamate (114 mg, 0.6 mmol). The mixture was stirred at room temperature under argon for 14 h and then concentrated under reduced pressure. The residue was diluted with EtOAc and washed with 1 M HCl_((aq)), saturated NaHCO_(3(aq)), and brine. The organic phase was dried over MgSO₄, filtered, and purified by silica gel chromatography (EtOAc/hexane=1:49) to afford the desired product 62 (153 mg, 80%). C₂₀H₃₀N₂O₃S; colorless oil; ¹H NMR (CDCl₃, 400 MHz) δ 12.20 (1H, s), 7.19 (2H, d, J=7.6 Hz), 7.08 (2H, d, J=6.0 Hz), 3.69 (1H, br), 4.41 (2H, d, J=7.2 Hz), 2.29 (3H, s), 1.81 (1H, sep, J=6.8 Hz), 1.50 (3H, d, J=7.2 Hz), 1.42 (9H, s), 0.86 (6H, d, J=6.4 Hz); HRMS (ESI) calcd for C₂₀H₃₁N₂O₃S: 379.2055, found: m/z 379.2060 [M+H]⁺.

Compound 63

To a solution of azide 59 (183 mg, 0.40 mmol) in MeOH (5 mL) was added Lindlar catalyst (91 mg). The mixture was stirred at room temperature under an atmosphere of hydrogen for 14 h. The resulting mixture was filtered through a pad of Celite, and the filtrate was concentrated under reduced pressure. The residue was dissolved in CH₂Cl₂ (5 mL), compound 62 (153 mg, 0.40 mmol), HgCl₂ (119 mg, 0.44 mmol), and Et₃N (61 μL, 0.44 mmol) were added. The mixture was stirred at room temperature under argon for 6H, filtered through a pad of Celite, and concentrated under reduced pressure. The residue was diluted with EtOAc, and washed with 1 M HCl_((aq)), saturated NaHCO_(3(aq)), and brine. The organic phase was dried over MgSO₄, filtered, and purified by silica gel chromatography (EtOAc/hexane, 1:1 to 7:3) to afford the product 63 (259 mg, 85%). C₃₇H₅₂N₄O₁₃; colorless oil; ¹H NMR (CDCl₃, 400 MHz) δ 12.12 (0.6H, s), δ 11.97 (0.4 H, s), 9.01 (0.6H, d, J=8.4 Hz), 8.40 (0.4 H, d, J=8.4 Hz), 7.22 (0.4H, d, J=8.4 Hz), 7.16 (0.6H, d, J=8.0 Hz), 7.09 (1H, d, J=8.0 Hz), 6.00 (0.6H, d, J=8.4 Hz), 5.88-5.82 (0.4H, m), 5.42-5.37 (0.6H, m), 5.32-5.22 (1.4H, m), 5.13-4.97 (1H, m), 4.67-4.58 (1H, m), 4.27-4.08 (3H, m), 3.80-3.71 (3H, m), 3.67-3.55 (1H, m), 2.50-2.35 (2H, m), 2.02-1.98 (9H, m), 1.88-1.75 (3H, m), 1.55-1.31 (13H, m), 0.92-0.78 (6H, m); HRMS (ESI) calcd for C₃₇H₅₃N₄O₁₃: 761.3609, found: m/z 761.3613 [M+H]⁺.

ZA-G-Ibu 47

To a solution of compound 63 (28 mg, 0.037 mmol) in CH₂Cl₂ (1 mL) was added TFA (1 mL). The mixture was stirred at room temperature for 1 h, and then concentrated under reduced pressure. The residue was dissolved in THF (2 mL), and 1 M KOH_((aq)) (2 mL) was added. The mixture was stirred at room temperature for 30 min, acidified by addition of TFA, and concentrated under reduced pressure. The residue was purified by reversed-phase chromatography (RP-18; 0-100% MeOH in H₂O) to afford ZA-G-Ibu 47 (18 mg, 95%). C₂₅H₃₆N₄O₈; colorless oil; ¹H NMR (CD₃OD, 400 MHz) δ 7.25 (2H, d, J=8.4 Hz), 7.12 (2H, d, J=7.6 Hz), 5.59 (1H, m), 4.62-4.52 (1H, m), 4.41-4.32 (1H, m), 4.32-4.20 (1H, m), 3.92-3.75 (3H, m), 3.71-3.58 (2H, m), 2.44 (2H, d, J=7.2 Hz), 1.93 (3H, s), 1.89-1.79 (1H, m), 1.46 (3H, d, J=6.8 Hz), 0.88 (6H, d, J=6.8 Hz); HRMS (ESI) calcd for C₂₅H₃₇N₄O₈: 521.2611, found: m/z 521.2612 [M+H]⁺.

Example 31 Synthesis of OS-1-CA Conjugate 48 (FIG. 24) Compound 46

To a solution of oseltamivir carboxylic acid N-Boc derivative (64, 60 mg, 0.16 mmol) in MeOH (0.5 mL) was added KOH (6.87 mg, 0.17 mmol). The mixture was stirred at room temperature for 30 min, and then concentrated under reduced pressure. The residue was dissolved in DMF (0.5 mL), and then added 3-iodo-1-propanol (17 mL, 0.19 mmol). After stirring at 50° C. for 4 h, the mixture was evaporated under reduced pressure. The residues was dissolved in CH₂Cl₂ (10 mL) and extracted with 1 M HCl and brine, dried over MgSO₄, concentrated under reduced pressure, and purified by flash silica gel column chromatography (EtOAc/hexane, 2:1) to afford compound 65 (50 mg, 72%). C₂₂H₃₈N₂O₇; white solid; mp 93-95° C.; TLC (EtOAc/n-hexane, 2:1) R_(f)=0.31; [α]_(D) ²²=−81.9 (c=1.0, CH₂Cl₂); IR (film) 3309, 2964, 2935, 2878, 1686, 1536, 1458, 1392, 1368, 1295, 1255, 1172, 1057 cm; ¹H NMR (400 MHz, CDCl₃) δ 6.77 (1H, s), 5.88 (1H, d, J=8.8 Hz), 5.11 (1H, d, J=9.6 Hz), 4.28-4.31 (2H, m), 4.01-4.08 (1H, m), 3.95 (1H, d, J=7.2 Hz), 3.73-3.82 (1H, m), 3.70 (2H, t, J=6.0 Hz), 3.30-3.34 (1H, m), 2.72 (1H, dd, J=17.6, 5.2 Hz), 2.26 (1H, dd, J=18.0, 10.0 Hz), 1.97 (3H, s), 1.87-1.93 (2H, m), 1.47-1.57 (4H, m), 1.41 (9H, s), 0.85-0.91 (6H, m); ¹³C NMR (100 MHz, CDCl₃) δ 170.9, 165.9, 156.2, 138.1, 128.8, 82.3, 79.5, 75.8, 61.8, 58.6, 54.6, 49.3, 31.5, 30.9, 28.4 (3×), 26.2, 25.7, 23.3, 9.7, 9.3; HRMS calcd for C₂₂H₃₉N₂O₇: 443.2757, found: m/z 443.2753 [M+H]⁺.

Compound 66

To a solution of the allyl ether of caffeic acid 18 (70 mg g, 0.27 mmol) in anhydrous CH₂Cl₂ (2.3 mL) were added NEt₃ (1.0 mL, 0.69 mmol), O-benzotriazole-N,N,N′,N′-tetramethyluronium hexafluorophosphate (HBTU, 103 mg, 0.27 mmol), and DMAP (catalytic amount). The mixture was stirred at room temperature for 30 min, and alcohol compound 46 (100 mg, 0.23 mmol) was added. The mixture was stirred for another 3 h at room temperature, and concentrated under reduced pressure. The residue was purified by flash chromatography on a silica gel column (EtOAc/hexane, 3:1) to afford the coupling product 47 (93 mg, 60%). C₃₇H₅₂N₂O₁₀; pale-yellow solid; TLC (EtOAc/hexane, 3:1) R_(f)=0.57; [α]_(D) ²⁵=−87.2 (c=1, CH₂Cl₂); IR (film) 3443, 2966, 1876, 1714, 1658, 1514, 1458, 1426, 1368, 1295, 1257, 1167, 1016 cm¹; ¹H NMR (400 MHz, CDCl₃) δ 7.58 (1H, d, J=16.0 Hz), 7.07 (2H, s), 6.86 (1H, d, J=8.8 Hz), 6.79 (2H, s), 6.24 (1H, d, J=16.0 Hz), 6.00-6.10 (2H, m), 5.39-5.44 (2H, m), 5.29 (2H, d, J=10.8 Hz), 5.04 (1H, d, J=8.8 Hz), 4.59-4.63 (4H, m), 4.27 (4H, t, J=6.4 Hz), 4.02-4.09 (1H, m), 3.83 (1H, br), 3.77-3.83 (1H, m), 3.31-3.34 (1H, m), 2.74 (1H, dd, J=18.4, 6.0 Hz), 2.25-2.32 (1H, m), 2.08 (2H, t, J=6.0 Hz), 1.97 (3H, s), 1.47-1.54 (4H, m), 1.41 (9H, s), 0.85-0.91 (6H, m); ¹³C NMR (100 MHz, CDCl₃) δ 170.5, 166.7, 165.5, 156.1, 150.4, 148.2, 144.7, 138.0, 132.8, 132.6, 128.8, 127.2, 122.5, 117.8, 117.7, 115.3, 113.2, 112.4, 82.1, 79.4, 75.7, 69.8, 69.6, 61.5, 60.8, 54.4, 49.1, 30.9, 28.3 (3×), 28.1, 26.1, 25.7, 23.3, 9.6, 9.3; HRMS calcd for C₃₇H₅₃N₂O₁₀: 685.3700, found: m/z 685.3725 [M+H]⁺.

OS-1-CA Conjugate 48

To a solution of compound 66 (50 mg, 0.07 mmol) in anhydrous THF (2 mL) were added Pd(PPh₃)₄ (23 mg, 0.02 mmol) and morpholine (2 mL). The mixture was stirred at room temperature for 4 h, and then extracted with 1 M HCl and brine. The organic phase was dried over MgSO₄, concentrated under reduced pressure, and purified by flash chromatography on a silica gel column (EtOAc/hexane, 2:1). The residue was dissolved in CH₂Cl₂ (2 mL) and TFA (2 mL). After stirred at room temperature for 3 h, the mixture was concentrated under reduced pressure. The residue was purified by reverse-phase silica gel chromatography (MeOH/H₂O, 0:10 to 10:0) to afford OS-1-CA conjugate 48 (25 mg, 70%). C₂₆H₃₆N₂O₈; white powder; TLC (2-propanol/H₂O/NH₄OH, 10:2:3) R_(f)=0.78; [α]_(D) ²⁴=−28.0 (c=1, MeOH); IR (film) 3263, 2966, 2936, 2878, 1679, 1600, 1523, 1443, 1374, 1272, 1249, 1201, 1182, 1135 cm¹; ¹H NMR (400 MHz, CD₃OD) δ 8.26 (1H, d, J=8.8 Hz), 7.53 (1H, d, J=15.6 Hz), 7.03 (1H, d, J=2.0 Hz), 6.94 (1H, dd, J=8.0, 2.0 Hz), 6.86 (1H, d, J=2.0 Hz), 6.78 (1H, d, J=8.0 Hz), 6.25 (1H, d, J=15.6 Hz), 4.24-4.35 (4H, m), 4.21 (1H, d, J=8.0 Hz), 3.94-3.99 (1H, m), 3.49-3.56 (1H, m), 3.39-3.44 (1H, m), 2.94 (1H, dd, J=17.6, 5.6 Hz), 2.47 (1H, tt, J=10.4, 2.8 Hz), 2.07-2.13 (2H, m), 2.04 (3H, s), 1.47-1.57 (4H, m), 0.92 (3H, t, J=6.4 Hz), 0.88 (3H, t, J=7.6 Hz); ¹³C NMR (100 MHz, CDCl₃) δ 175.0, 169.3, 166.9, 149.8, 147.3, 147.0, 139.5, 128.4, 127.8, 123.2, 116.7, 115.4, 115.0, 83.9, 75.8, 63.3, 62.3, 54.4, 50.8, 29.6, 29.3, 27.3, 26.8, 23.3, 10.0, 9.7; HRMS calcd for C₂₆H₃₆N₂O₈: 505.2550, found: m/z 505.2553 [M+H]⁺.

Example 32 Synthesis of TP-1-CA Conjugate 49 (FIG. 25) Compound 68

To a solution of phosphorus trichloride (1.57 g, 11.4 mmol) in CH₂Cl₂ (114 mL) was added 1,3-propandiol (0.9 g, 12 mmol) dropwise at 0° C. The mixture was stirred for 8 h, and then washed twice with brine. The organic layer was dried over MgSO₄, and concentrated under reduced pressure to give 2-oxo-1,3,2-dioxaphosphorinane (68, 0.93 g, 66%). C₃H₇O₃P; colorless oil; ¹H NMR (400 MHz, CDCl₃) δ 6.87 (1H, d, J=675.6 Hz), 4.20-4.49 (2H, m), 2.20-2.31 (1H, m), 1.75-1.82 (1H, m); ¹³C NMR (100 MHz, CDCl₃) δ 67.1 (2×, d, J_(C-P)=6.8 Hz), 25.5 (d, J_(C-P)=8.4 Hz); ³³P NMR (162 MHz, CDCl₃) δ 3.47.

Compound 69

A mixture of iodine compound 67 (50 mg, 0.11 mmol), phosphorinane 68 (20 mg, 0.22 mmol) and Et₃N (46 μL, 0.32 mmol) in anhydrous toluene (1.0 mL) was deoxygenated by bubbling with nitrogen for 10 min, and then added to tetrakis(triphenylphosphine)palladium(0) (10 mg, 8.6 mmol) that was placed in a round-bottomed flask under nitrogen atmosphere. The mixture was gradually heated to 90° C. and maintained at this temperature for 12 h. The mixture was filtered through a pad of Celite, and the filtrate was concentrated under reduced pressure to give yellow foam (98 mg), which was purified by flash column chromatography on a silica gel column (EtOAc/hexane, 1:1) to afford a crude product of phosphonate 69 (77 mg).

Compound 51

The crude product 69 was dissolved in ethanol (1.1 mL), and treated with KOH (1.1 mL of 1 M aqueous solution) at 25° C. for 0.5 h. The mixture was neutralized with Dowex 50W×8, filtered, and concentrated under reduced pressure. The residue was purified on a C-18 column. The product was dissolved in NH₄OH, stirred at room temperature for 0.5 h, and then lyophilized to afford compound 70 as the ammonium salt (43 mg, 83%). C₂₁H₃₉N₂O₈P; white solid; mp 174-178° C.; TLC (MeOH/CH₂Cl₂, 1:10) R_(f)=0.10; [α]_(D) ¹⁹=−46.9 (c=0.9, MeOH); IR (film) 3325, 2965, 2937, 1660, 1541, 1458, 1369, 1296, 1256, 1184 cm¹; ¹H NMR (400 MHz, CD₃OD) δ 6.52 (1H, J=21.2 Hz), 4.03-4.08 (3H, m), 3.86 (1H, t, J=11.2 Hz), 3.69-3.76 (1H, m), 3.67 (2H, t, J=6.8 Hz), 3.39-3.42 (1H, m), 2.54-2.58 (1H, s), 2.22-2.28 (1H, s), 1.96 (3H, s), 1.84-1.90 (2H, m), 1.48-1.56 (4H, m), 1.43 (9H, s), 0.86-0.94 (6H, s); ¹³C NMR (100 MHz, CD₃OD) δ 174.8, 173.8, 158.2, 137.8 (C-1, d, J_(C-P)=118 Hz), 83.6, 80.2, 76.5 (d, J_(C-P)=18.7 Hz), 63.4 (d, J_(C-P)=5.3 Hz), 54.7, 50.8 (d, J_(C-P)=12.0 Hz), 34.9, 34.86, 34.3 (d, J_(C-P)=6.7 Hz), 28.9 (3×), 26.8, 23.3, 10.02, 10.0; ³¹P NMR (162 MHz, CD₃OD) δ 15.6; HRMS calcd for C₂₁H₃₈N₂O₈P (M−H): 477.2366, found: m/z 477.2368.

Compound 71

To a solution of the allyl ether of caffeic acid 18 (160 mg, 0.61 mmol) in anhydrous CH₂Cl₂ were added N-hydroxysuccinimide (84 mg, 0.74 mmol), HBTU (280 mg, 0.74 mmol), DMAP (16 mg, 10% w/w) and diisopropylethylamine (0.4 mL, 1.8 mmol). The mixture was stirred at room temperature for 2H, concentrated, and washed with H₂O. The residue was purified by silica gel chromatography (EtOAc/hexane=1:3) to give compound 71 (164 mg, 75%). C₁₉H₁₉NO₆; TLC (EtOAc/hexane=1:2) R_(f)=0.26; ¹H NMR (400 MHz, CDCl₃) δ 7.80 (1H, d, J=16.0 Hz), 7.10 (2H, td, J=8.4, 2.0 Hz), 6.87 (1H, d, J=8.4 Hz), 6.38 (1H, d, J=16.0 Hz), 6.00-6.10 (2H, m), 5.41 (2H, dt, J=17.2, 1.2 Hz), 5.29 (2H, dd, J=10.8, 1.2 Hz), 4.61-4.64 (4H, m), 2.85 (4H, s); ¹³C NMR (100 MHz, CDCl₃) δ 168.7 (2×), 161.6, 151.1, 149.4, 148.0, 132.4, 132.1, 126.1, 123.5, 117.8, 117.7, 112.8, 112.4, 108.5, 69.9, 69.6, 26.0 (2×).

Compound 72

A solution of alcohol compound 70 (65 mg, 0.14 mmol) and caffeic acid activated ester 71 in anhydrous DMF (2.4 mL) was stirred at room temperature for 16 h. The mixture was concentrated, and the residue was purified on an RP-18 reverse-phase column (H₂O/MeOH=6:4 to 5:5) to give the coupling product 72 (66 mg, 67%). C₃₆H₅₃N₂O₁₁P; TLC (MeOH/CH₂Cl₂=1:10) R_(f)=0.17; ¹H NMR (400 MHz, CD₃OD) δ 7.43 (1H, d, J=16.0 Hz), 7.14 (1H, s), 7.09 (1H, d, J=8.4 Hz), 6.94 (1H, d, J=8.4 Hz), 6.50 (1H, d, J=19.2 Hz), 6.41 (1H, d, J=16.0 Hz), 6.02-6.11 (2H, m), 5.42 (2H, d, J=17.2 Hz), 5.25 (2H, d, J=10.8 Hz), 4.59 (4H, d, J=4.8 Hz), 4.18-4.23 (1H, m), 4.12-4.14 (1H, m), 3.96-4.01 (3H, m), 3.66-3.74 (2H, m), 3.41-3.44 (1H, m), 2.62-2.66 (1H, m), 2.35-2.37 (1H, m), 1.89 (3H, s), 1.81-1.87 (2H, m), 1.46-1.56 (4H, m), 1.30-1.37 (4H, m), 0.92 (3H, t, J=7.6 Hz), 0.87 (3H, t, J=7.6 Hz); ¹³C NMR (100 MHz, CD₃OD) δ 173.7, 168.6, 163.7, 150.7 (C-1, d, ¹J_(C-P)=172.5 Hz), 141.8, 134.8, 134.7, 134.5, 129.4, 125.0, 123.5, 119.7, 117.9, 117.3, 115.0, 114.0, 83.6, 77.8, 71.1 (d, ²J_(C-P)=6.8 Hz), 70.8, 62.8, 59.4, 56.5, 47.9 (3×), 34.9, 32.8, 29.0, 27.6, 27.0, 23.1, 10.2, 9.9, 9.4; ³¹P NMR (162 MHz, CD₃OD) δ 13.4.

TP-1-CA Conjugate 49

A solution of 72 (100 mg, 0.14 mmol) in anhydrous toluene (1.0 mL) was deoxygenated by bubbling with nitrogen for 10 min, and then added to tetrakis(triphenylphosphine)palladium(0) (10 mg, 8.6 μmol) that was placed in a round-bottomed flask under nitrogen atmosphere. The mixture was stirred at 25° C. for 8 h, and then filtered through Celite. The filtrate was concentrated under reduced pressure to give yellow foam, which was dissolved in CH₂Cl₂ (0.50 mL) and treated with TFA (0.10 mL, 1.4 mmol). After stirring for 3H, the mixture was concentrated under reduced pressure. The residue was dissolved in NH₄OH, stirred at room temperature for 0.5 h, and purified on a C-18 column, followed by lyophilization, to afford TP-1-CA conjugate 49 (48 mg, 64%). C₂₅H₃₇N₂O₉P; ¹H NMR (400 MHz, CD₃OD) δ 7.36 (1H, d, J=15.6 Hz), 7.00 (1H, d, J=2.0 Hz), 6.89 (1H, dd, J=8.0, 1.6 Hz), 6.76 (1H, d, J=8.4 Hz), 6.45 (1H, br), 6.31 (1H, d, J=15.6 Hz), 4.19-4.27 (1H, m), 4.10-4.12 (1H, m), 3.78-3.89 (2H, m), 3.42-3.45 (1H, m), 3.21 (2H, t, J=4.8 Hz), 2.63 (1H, br), 2.35 (1H, br), 1.90 (3H, s), 1.75 (2H, m, J=6.8 Hz), 1.43-1.58 (4H, m), 0.93 (3H, t, J=7.6 Hz), 0.88 (3H, t, J=7.6 Hz); ¹³C NMR (100 MHz, CD₃OD) δ 174.0, 168.9, 150.9 (C-1, d, J_(C-P)=172.8 Hz), 138.9, 134.8 (d, J_(C-P)=18.3 Hz), 129.6, 123.6, 119.9, 115.2, 114.2, 83.7 (d, J_(C-P)=7.8 Hz), 62.8, 59.4, 56.5, 34.8, 32.7, 28.9, 27.5, 26.9, 23.1, 10.1, 9.8, 9.3; ³¹P NMR (162 MHz, CD₃OD) δ 14.3; HRMS calcd for C₂₅H₃₆N₂O₉P (M−H): 539.2158, found: m/z 539.2147.

Example 33 Synthesis of PZA(1Et)-7-CA-Amide Conjugate 50 (FIG. 26) 3-Acetamido-4,6-diacetoxy-2-(1,2,3-triacetoxy)propyl-3,4,5,6-tetrahydro-2H-pyran (73)

Under an atmosphere of nitrogen, a suspension of N-acetylneuraminic acid (5 g, 16.2 mmol) in pyridine (75 mL) and acetic anhydride (75 mL) was stirred at room temperature for 12 h, and then heated at 100° C. for 5 h. The reaction mixture was cooled to room temperature, and concentrated under reduced pressure. The residual brownish glassy oil was dissolved in CH₂Cl₂ (150 mL), and washed successively with saturated aqueous NaHCO₃ (100 mL), aqueous 1 M HCl (100 mL) and brine (100 mL). The organic layer was dried over MgSO₄, filtered and concentrated. The brownish residue was purified by column chromatography on silica gel (EtOAc/hexane, 67:33 to 100:0) to afford 73 as a pale yellow foam (3.8 g, 50%), which contained inseparable mixture of anomers (α/β=1:5). The anomeric mixture of 73 was used in the next step without further separation. C₂₀H₂₉NO₁₂; TLC (EtOAc) R_(f)=0.35; ¹H NMR (600 MHz, CDCl₃) δ 6.26 (0.83 H, d, J=2.5 Hz, H-1β), 5.62 (0.17 H, dd, J=10.3, 2.1 Hz, H-1a), 5.43 (0.17 H, ddd, J=6.1, 4.4, 1.9 Hz), 5.29-5.27 (1.66 H, m), 5.22 (0.83 H, td, J=10.6, 4.9 Hz), 5.17 (0.83 H, td, J=6.5, 2.7 Hz), 5.11-5.07 (0.34 H, m), 5.03 (0.17 H, dd, J=6.5, 2.7 Hz), 4.36 (0.17 H, dd, J=12.5, 2.6 Hz), 4.31 (0.83 H, dd, J=12.5, 2.8 Hz), 4.08-3.98 (2.83 H, m), 3.74 (0.17 H, dd, J=10.5, 2.5 Hz), 2.17-2.15 (0.17 H, m), 2.15-2.13 (0.83 H, m), 2.11 (2.49 H, s), 2.10 (0.51H, s), 2.09 (0.51 H, s), 2.08 (2.49 H, s), 2.07 (0.51 H, s), 2.04 (2.49 H, s), 2.03 (0.51 H, s), 2.017 (2.49H, s), 2.013 (0.51 H, s), 2.00 (2.49 H, s), 2.00-1.98 (0.83 H, m), 1.98-1.96 (0.17 H, m), 1.88 (2.49 H, s), 1.87 (0.51 H, s); HRMS calcd for C₂₀H₂₉NO₁₂Na: 498.1587, found: m/z 498.1557 [M+Na]⁺.

Diethyl [5-acetamido-4-acetoxy-6-(1,2,3-triacetoxy)propyl-3,4,5,6-tetrahydro-2H-pyran-2-yl]phosphonate (74)

The anomeric mixture of 73 (2.15 g, 4.52 mmol) and diethyl trimethylsilyl phosphite (3.11 mL, 13.65 mmol) in anhydrous CH₂Cl₂ (30 mL) was treated with trimethylsilyl trifluoromethylsulfonate (TMSOTf, 1.23 mL, 6.78 mmol) at 0° C. After 30 min, the mixture was warmed to room temperature, and stirred for 24 h. The mixture was poured into ice water (20 mL), and the aqueous layer was extracted with CH₂Cl₂ (20 mL, 2×). The combined extracts were washed successively with saturated aqueous NaHCO₃ (50 mL) and brine (50 mL), dried over MgSO₄, filtered and concentrated. The residue was purified by column chromatography on silica gel (acetone/EtOAc, 1:9) to afford 74 as a colorless syrup (1.55 g, 62%), which contained a mixture of the α- and β-anomers (2:3). The anomeric mixture of phosphonate 74 was used in the next step without further separation. The analytical samples of pure α- and β-anomers (74α and 74β) were obtained by flash column chromatography on silica gel (EtOAc/acetone, 100:0 to 90:10). α-Anomer 74a: C₂₂H₃₆NO₁₃P; colorless foam; TLC (EtOAc/acetone, 9:1) R_(f)=0.25; [α]_(D) ²⁰=+39.4 (c=4.6, CH₂Cl₂); ¹H NMR (600 MHz, CDCl₃) δ 5.30 (1H, dd, J=5.7, 1.7 Hz), 5.24 (1H, d, J=9.9 Hz, NH), 5.18 (1H, td, J=6.6, 2.5 Hz), 4.98 (1H, td, J=10.6, 5.0 Hz), 4.40 (1H, dd, J=12.3, 5.0 Hz), 4.22-4.09 (5H, m), 3.97 (1H, q, J=10.1 Hz), 3.74 (1H, td, J=12.5, 2.4 Hz), 3.62 (1H, dd, J=10.3, 2.0 Hz), 2.27 (1H, dd, J=12.8, 4.9 Hz), 2.09 (3H, s), 2.05 (3H, s), 2.02 (3H, s), 2.01 (3H, s), 1.98-1.92 (1H, m), 1.87 (3H, s), 1.35-1.31 (6H, m); ¹³C NMR (150 MHz, CDCl₃) δ 170.9 (C), 170.5 (C), 170.3 (C), 170.2 (C), 170.1 (C), 79.0 (CH, d, ³J_(C-P)=17.3 Hz), 71.8 (CH, d, J_(C-P)=174.6 Hz, C-1), 71.6 (CH, d, ³J_(c-p)=20.9 Hz), 71.0 (CH), 67.9 (CH), 63.4 (CH₂, d, ²J_(C-P)=6.9 Hz, POCH₂), 62.8 (CH₂, d, ²J_(C-P)=6.2 Hz, POCH₂), 62.2 (CH₂, C-8), 49.6 (CH, C-4), 31.3 (CH₂, C-2), 23.1 (CH₃), 20.9 (CH₃), 20.8 (CH₃), 20.7 (CH₃, 2×), 16.5 (CH₃, d, ³J_(c-p)=5.4 Hz, POCH₂ CH₃), 16.3 (CH₃, d, ³J_(c-p)=5.4 Hz, POCH₂CH₃); ³¹P NMR (202 MHz, CDCl₃) δ 18.5; HRMS calcd for C₂₂H₃₅NO₁₃P: 552.1846, found: m/z 552.1921 [M−H]. 3-Anomer 743: C₂₂H₃₆NO₁₃P; colorless foam; TLC (EtOAc/acetone, 9:1) R_(f)=0.28; [α]_(D) ²⁰=−40.1 (c=3.0, CH₂Cl₂); ¹H NMR (600 MHz, CDCl₃) δ 5.45 (1H, d, J=10.1 Hz, NH), 5.35 (1H, dd, J=7.3, 2.3 Hz), 5.32 (1H, td, J=15.0, 4.8 Hz), 5.21-5.18 (1H, m), 4.45 (1H, d, J=10.0 Hz), 4.33 (1H, dd, J=12.4, 2.8 Hz), 4.30 (1H, dd, J=12.3, 7.1 Hz), 4.19-4.13 (2H, m), 4.12-4.04 (4H, m), 2.35-2.31 (1H, m), 2.11 (3H, s), 2.08 (3H, s), 2.017 (3H, s), 2.011 (3H, s), 2.09-2.03 (1H, m), 1.88 (3H, s), 1.34 (3H, t, J=7.0 Hz), 1.33 (3H, t, J=7.0 Hz); ¹³C NMR (150 MHz, CDCl₃) δ 170.8 (C), 170.6 (C), 170.2 (C), 170.1 (C), 169.8 (C), 74.0 (CH), 69.7 (CH), 69.5 (CH), 67.9 (CH, d, ¹J_(c-p)=157.2 Hz, C-1), 67.7 (CH), 63.0 (CH₂, d, ²J_(c-p)=7.2 Hz, POCH₂), 62.7 (CH₂, d, ²J_(c-p)=6.6 Hz, POCH₂), 62.0 (CH₂, C-8), 49.0 (CH, C-4), 29.5 (CH₂, d, ²J_(c-p)=3.2 Hz, C-2), 23.1 (CH₃), 21.0 (CH₃), 20.9 (CH₃), 20.7 (CH₃, 2×), 16.2 (CH₃, d, ³J_(c-p)=5.1 Hz, POCH₂ CH₃), 16.3 (CH₃, d, ³J_(c-p)=5.1 Hz, POCH₂CH₃); ³¹P NMR (202 MHz, CDCl₃) δ 21.4; HRMS calcd for C₂₂H₃₅NO₁₃P: 552.1846, found: m/z 552.1879 [M−H].

Diethyl [5-acetamido-4-acetoxy-6-(1,2,3-triacetoxy)propyl-4,5,6-trihydropyran-2-yl]phosphonate (75)

The anomeric mixture of phosphonate 74 (1.1 g, 2 mmol) and N-bromosuccinimide (885 mg, 5 mmol) in anhydrous CH₂Cl₂ (20 mL) was heated to reflux under irradiation from a 100 W tungsten lamp. The progress of reaction was monitored by TLC. On completion (˜6 h) the mixture was cooled to room temperature, and the precipitate succinimide was filtered off. The filtrate was evaporated under reduced pressure to give a crude 2-bromo derivative as yellow syrup, which was used in the next step without further purification.

A solution of the above-prepared bromine compound in anhydrous pyridine (10 mL) was stirred at 50° C. for 2 h. The solution was concentrated under reduced pressure, and the brown residue was purified by column chromatography on silica gel (EtOAc/acetone, 100:0 to 90:10) to afford conjugated phosphonate 75 as colorless foam (827 mg, 75% for two steps). C₂₂H₃₄NO₁₃P; TLC (EtOAc/acetone, 9:1) R_(f)=0.28; [α]_(D) ²⁰=+43.8 (c=0.59, CH₂Cl₂); ¹H NMR (600 MHz, CDCl₃) δ 5.74 (1H, dd, J=10.7, 2.2 Hz), 5.54 (1H, d, J=8.2 Hz, NH), 5.42-5.40 (2H, m), 5.26 (1H, td, J=6.4, 2.9 Hz), 4.39-4.34 (2H, m), 4.29 (1H, q, J=9.1 Hz), 4.17-4.09 (5H, m), 2.09 (3H, s), 2.05 (3H, s), 2.04 (3H, s), 2.02 (3H, s), 1.91 (3H, s), 1.35 (3H, t, J=7.0 Hz), 1.31 (3H, t, J=7.0 Hz); ¹³C NMR (150 MHz, CDCl₃) δ 170.8 (C), 170.4 (C), 170.3 (C), 169.8 (C), 169.7 (C), 147.8 (C, d, J_(c-p)=225 Hz, C-1), 113.0 (CH, d, ²J_(c-p)=22.8 Hz, C-2), 76.5 (CH, d, ³J_(c-p)=9.3 Hz), 69.9 (CH), 68.4 (CH, d, ³J_(c-p)=15.2 Hz), 67.2 (CH), 63.2 (CH₂, d, ²J_(c-p)=5.4 Hz, POCH₂), 63.0 (CH₂, d, ²J_(c-p)=5.7 Hz, POCH₂), 61.8 (CH₂, C-8), 46.4 (CH, C-4), 23.0 (CH₃), 20.78 (CH₃), 20.73 (CH₃), 20.63 (CH₃), 20.60 (CH₃), 16.16 (CH₃, d, ³J_(c-p)=4.8 Hz, POCH₂CH₃), 16.12 (CH₃, d, ³J_(c-p)=4.8 Hz, POCH₂CH₃); ³¹P NMR (202 MHz, CDCl₃) δ 6.4; HRMS calcd for C₂₂H₃₃NO₁₃P: 550.1690, found: m/z 550.1684 [M−H].

Diethyl [4-(1,2,3-triacetoxy)propyl-2-methyl-3a,7a-dihydro-4H-pyrano[3,4-d]oxazol-6-yl]phosphonate (76)

To a solution of phosphonate 75 (550 mg, 1 mmol) in a mixture of acetic acid (2 mL) and acetic anhydride (2 mL) was treated with conc. H₂SO₄ (0.2 mL). The mixture was stirred for 48 h at room temperature, poured into cold (0° C.) saturated aqueous NaHCO₃ (pH 9), and stirred for 30 min before extraction with EtOAc (30 mL, 5×). The combined extracts were dried over MgSO₄, filtered and concentrated under reduced pressure. The residual oil was purified by column chromatography on silica gel (acetone/EtOAc, 1:9) to afford 76 as pale yellow syrup (394 mg, 80% for two steps). C₂₀H₃₀NO₁₁P; TLC (EtOAc/acetone, 9:1) R_(f)=0.30; [α]_(D) ²⁰=−11.6 (c=0.50, CH₂Cl₂); ¹H NMR (600 MHz, CDCl₃) δ 6.20 (1H, dd, J=10.3, 4.0 Hz), 5.58 (1H, ddd, J=6.6, 2.9, 1.1 Hz), 5.38 (1H, td, J=7.7, 2.5 Hz), 4.71 (1H, ddd, J=8.6, 4.0, 2.0 Hz), 4.40 (1H, dd, J=12.4, 2.5 Hz), 4.19 (1H, dd, J=12.5, 5.9 Hz), 4.18-4.07 (4H, m), 3.93 (1H, td, J=9.2, 0.6 Hz), 3.34 (1H, dd, J=10.1, 2.7 Hz), 2.11 (3H, s), 2.04 (3H, s), 2.03 (3H, s), 1.98 (3H, s), 1.34 (3H, t, J=7.0 Hz), 1.32 (3H, t, J=7.0 Hz); ¹³C NMR (150 MHz, CDCl₃) δ 170.5 (C), 169.7 (C), 169.4 (C), 167.2 (C, N═CCH₃), 150.1 (C, d, J_(c-p)=225 Hz, C-1), 111.9 (CH, d, ²J_(c-p)=23.4 Hz, C-2), 76.1 (CH, d, ³J_(c-p)=6.3 Hz), 71.2 (CH, d, ³J_(c-p)=15.3 Hz), 69.6 (CH), 68.8 (CH), 63.1 (CH₂, d, ²J_(c-p)=5.9 Hz, POCH₂), 62.9 (CH₂, d, ²J_(c-p)=5.7 Hz, POCH₂), 61.8 (CH, C-4), 61.6 (CH₂, C-8), 20.7 (CH₃), 20.6 (CH₃), 20.5 (CH₃), 16.2 (CH₃, d, ³J_(c-p)=5.1 Hz, POCH₂CH₃), 16.1 (CH₃, d, ³J_(c-p)=5.1 Hz, POCH₂CH₃), 14.0 (CH₃, N═CCH₃); ³¹P NMR (202 MHz, CDCl₃) δ 6.4; HRMS calcd for C₂₀H₂₉NO₁₁: 490.1478, found: m/z 490.1374 [M−H]⁻.

Diethyl [5-acetamido-4-azido-6-(1,2,3-triacetoxy)propyl-4,5,6-trihydropyran-2-yl]phosphonate (77)

To a solution of oxazoline 76 (393 mg, 0.8 mmol) in t-BuOH (10 mL) was treated with azidotrimethylsilane (0.53 mL, 4 mmol) at 80° C. for 24 h. The solution was poured into saturated aqueous NaHCO₃, and extracted with EtOAc (30 mL, 3×). The combined extracts were dried over MgSO₄, filtered and concentrated to afford the azido compound 77 as a colorless syrup (371 mg, 87%), which was practically pure to be used in the next step. An analytical sample was obtained by flash column chromatography on silica gel (10% acetone in EtOAc). C₂₀H₃₁N₄O₁₁P; TLC (EtOAc/acetone, 9:1) R_(f)=0.30; [α]_(D) ²⁰=+82.7 (c=0.58, CH₂Cl₂); ¹H NMR (600 MHz, CDCl₃) δ 5.75 (1H, dd, J=10.3, 2.4 Hz), 5.73 (1H, d, J=8.6 Hz), 5.38 (1H, dt, J=7.1, 1.5 Hz), 5.26 (1H, ddd, J=8.5, 5.8, 2.6 Hz), 4.53-4.50 (2H, m), 4.36 (1H, dd, J=12.5, 2.6 Hz), 4.17-4.08 (5H, m), 3.67 (1H, q, J=9.2 Hz), 2.10 (3H, s), 2.05 (3H, s), 2.02 (3H, s), 1.99 (3H, s), 1.34 (3H, t, J=7.1 Hz), 1.32 (3H, t, J=7.1 Hz); ¹³C NMR (150 MHz, CDCl₃) δ 170.8 (C), 170.5 (C), 170.1 (C), 169.7 (C), 147.7 (C, d, ¹J_(c-p)=224 Hz, C-1), 112.4 (CH, d, ²J_(c-p)=22.9 Hz, C-2), 75.9 (CH, d, ³J_(c-p)=9.2 Hz), 69.7 (CH), 67.3 (CH), 63.5 (CH₂, d, ²J_(c-p)=5.7 Hz, POCH₂), 63.3 (CH₂, d, ²J_(c-p)=5.9 Hz, POCH₂), 61.9 (CH₂, C-8), 57.8 (CH, d, ³J_(c-p)=14.7 Hz), 48.5 (CH, C-4), 23.2 (CH₃), 20.8 (CH₃), 20.77 (CH₃), 20.71 (CH₃), 16.27 (CH₃, d, ³J_(c-p)=5.7 Hz, POCH₂CH₃), 16.23 (CH₃, d, ³J_(c-p)=5.7 Hz, POCH₂CH₃); ³¹P NMR (202 MHz, CDCl₃) δ 6.2; HRMS calcd for C₂₀H₃₀N₄O₁₁P: 533.1649, found: m/z 533.1540 [M−H].

[5-acetamido-4-tert-butoxycarbonylamino-6-(1,2,3-triacetoxy)propyl-4,5,6-trihydropyran-2-yl]phosphonate (78)

To a solution of azide 77 (249 mg, 0.47 mmol) in ethanol (21 mL) was hydrogenated with Lindlar catalyst (110 mg) under an atmosphere of hydrogen. The mixture was stirred for 12 h, filtered through a pad of Celite, and washed with methanol. The filtrate was concentrated under reduced pressure to give a colorless foam (240 mg). The crude amine product was dissolved in CH₂Cl₂ (20 mL) and treated with Boc₂O (171 mg, 0.78 mmol) and Et₃N (191 μL, 1.42 mmol). The reaction was stirred at room temperature for 12 h, and then washed with 1 M HCl (aq) and brine, dried over MgSO₄, filtered and concentrated. The residue was purified by flash column chromatography (EtOAc) to afford 78 as a white foam (204 mg, 72%). C₂₅H₄₁N₂O₁₃P; TLC (EtOAc) R_(f)=0.25; ¹H NMR (400 MHz, CDCl₃) δ 5.75 (1H, d, J=9.3 Hz), 5.69 (1H, dd, J=10.5, 1.7 Hz), 5.40 (1H, d, J=6.4 Hz), 5.22 (1H, td, J=6.4, 2.6 Hz), 4.71 (1H, d, J=9.5 Hz), 4.42-4.36 (2H, m), 4.21-4.07 (7H, m), 2.07 (3H, s), 2.06 (3H, s), 2.02 (3H, s), 1.90 (3H, s), 1.38 (9H, s), 1.35-1.32 (6H, m); ¹³C NMR (100 MHz, CDCl₃) δ 171.0, 170.5, 169.9, 169.8, 156.2, 147.1 (d, ¹J_(c-p)=225 Hz), 115.2 (d, ²J_(c-p)=22.8 Hz), 80.3, 77.6 (d, ³J_(c-p)=9.9 Hz), 70.2, 67.5, 63.2 (d, ²J_(c-p)=5.4 Hz), 63.0 (d, ²J_(c-p)=6.1 Hz), 62.0, 49.9 (d, ³J_(c-p)=14.4 Hz), 48.0, 28.2, 23.2, 20.9, 20.7, 20.6, 16.3, 16.2; 3P NMR (162 MHz, CDCl₃) δ 6.1; HRMS calcd for C₂₅H₄₀N₂O₁₃P: 607.2268, found: m/z 607.2269 [M−H]⁻.

[5-acetamido-4-tert-butoxycarbonylamino-6-[(2,2-dimethyl-[1,3]dioxolan-4-yl)-hydroxy-methyl]-5,6-dihydropyran-2-yl]phosphonate (79)

To a solution of 78 (181 mg, 0.30 mmol) in ethanol (9 mL) was added NaOEt (20 mg, 0.29 mmol). The mixture was stirred at room temperature for 0.5 h, neutralizes by Dowex 50W×8 (H⁺), filtered, and concentrated under reduced pressure to afford white solids. The residue was dissolved in acetone (4 mL), and p-toluenesulfonic acid and 2,2-dimethoxypropane were added. The mixture was stirred at room temperature for 16 h and then quenched the reaction with Et₃N. After concentration, the residue was purified by silica gel column chromatography (EtOAc) to afford 79 (129 mg, 83%). C₂₂H₃₉N₂O₁₀P; Colorless foam; TLC (EtOAc) R_(f)=0.33; ¹H NMR (400 MHz, CDCl₃) δ 6.65 (1H, d, J=6.5 Hz), 5.62 (1H, d, J=10.0 Hz), 5.01 (1H, br), 4.82 (1H, d, J=8.6 Hz), 4.54 (1H, br), 4.24-4.19 (1H, m), 4.17-4.11 (5H, m), 4.01-3.95 (2H, m), 3.87 (1H, m), 3.45 (1H, d, J=7.6 Hz), 2.02 (3H, s), 1.42 (9H, s), 1.37-1.30 (12H, m); ¹³C NMR (100 MHz, CDCl₃) δ 173.8, 157.3, 147.9 (d, ¹J_(c-p)=220 Hz), 113.7 (d, ²J_(c-p)=24.0 Hz), 109.0, 80.5, 78.8 (d, ³J_(c-p)=9.0 Hz), 73.9, 70.0, 67.7, 63.3 (d, ²J_(c-p)=6.0 Hz), 63.1 (d, ²J_(c-p)=6.0 Hz), 51.8, 48.7 (d, ³J_(c-p)=15.0 Hz), 28.2, 26.9, 25.2, 22.9, 16.2-16.1 (2×); ³¹P NMR (162 MHz, CDCl₃) δ 5.1; HRMS calcd for C₂₂H₃₈N₂O₁₀P: 521.2264, found: m/z 521.2267 [M−H]⁻.

Diethyl [5-acetamido-4-tert-butoxycarbonylamino-6-[(2,2-dimethyl-[1,3]dioxolan-4-yl)-(4-nitro-phenoxycarbonyloxy)-methyl]-5,6-dihydropyran-2-yl]phosphonate (80)

A solution of 79 (129 mg, 0.25 mmol), 4-nitrophenyl chloroformate (127 mg, 0.63 mmol) in pyridine (4 mL) was stirred at room temperature for 18 h. After concentration, the residue was purified by silica gel column chromatography (EtOAc/hexane=1:3 to EtOAc) to afford 80 (130 mg, 77%). C₂₉H₄₂N₃O₁₄P; TLC (EtOAc:acetone=9:1) R_(f)=0.48; Colorless solid; ¹H NMR (400 MHz, CDCl₃) δ 8.25 (2H, d, J=9.2 Hz), 7.49 (2H, d, J=9.0 Hz), 5.96 (1H, d, J=9.5 Hz), 5.71 (1H, dd, J=10.4, 1.9 Hz), 5.23 (1H, d, J=5.2 Hz), 4.76 (1H, m), 4.51-4.46 (1H, m), 4.37-4.29 (2H, m), 4.21 (1H, d, J=10.1 Hz), 4.18-4.07 (5H, m), 4.02 (1H, dd, J=9.0, 6.8 Hz), 1.94 (3H, s), 1.39 (9H, s), 1.39-1.32 (12H, m); ¹³C NMR (100 MHz, CDCl₃) δ 171.1, 156.3, 155.7, 152.4, 146.6 (d, J_(c-p)=220 Hz), 145.5, 125.2, 122.3, 115.7 (d, ²J_(c-p)=23.0 Hz), 108.8, 80.3, 77.7 (d, ³J_(c-p)=9.7 Hz), 74.9, 74.3, 65.5, 63.1 (d, ²J_(c-p)=5.0 Hz), 62.9 (d, ²J_(c-p)=6.0 Hz), 60.3, 49.7 (d, ³J_(c-p)=15.0 Hz), 28.1, 26.2, 25.3, 23.1, 16.2 (d, ³J_(c-p)=6.7 Hz), 16.2 (d, ³J_(c-p)=6.2 Hz); ³¹P NMR (162 MHz, CDCl₃) δ 6.3; HRMS calcd for C₂₉H₄₃N₃O₁₄P: 688.2483, found: m/z 688.2482 [M+H]⁺.

Compound 82

To a solution of compound 81 (47 mg, 0.20 mmol) in CH₃CN (5 mL) were added compound 80 (120 mg, 0.17 mmol) and Et₃N (0.094 mL, 0.70 mmol). The mixture was stirred at room temperature for 12 h, and then concentrated under reduced pressure. The residue was purified by silica gel column chromatography (MeOH/CH₂Cl₂=1:9) to afford product 82 (98 mg, 72%). C₃₅H₅₃N₄O₁₄P; White solid; TLC (MeOH/CH₂Cl₂=1:9) R_(f)=0.25; ¹H NMR (400 MHz, CD₃OD) δ 7.39 (1H, d, J=16.2 Hz), 7.00 (1H, d, J=1.7 Hz), 6.90 (1H, dd, J=8.5, 2.1 Hz), 6.77 (1H, d, J=8.5 Hz), 6.37 (1H, d, J=15.8 Hz), 5.66 (1H, d, J=9.4 Hz), 5.20 (1H, d, J=6.4 Hz), 4.39-4.36 (2H, m), 4.30 (1H, q, J=6.1 Hz), 4.20-4.10 (4H, m), 4.08-4.04 (2H, m), 3.96 (1H, dd, J=8.6, 6.4 Hz), 3.39-3.33 (3H, m), 3.20-3.06 (2H, m), 1.94 (3H, s), 1.80-1.67 (2H, m), 1.41 (9H, s), 1.38-1.29 (12H, m); ¹³C NMR (100 MHz, CD₃OD) δ 173.3, 169.3, 158.1, 157.7, 147.7 (d, ¹J_(c-p)=201 Hz), 142.1, 128.3, 122.1, 118.5, 116.5, 115.0, 110.2, 80.5, 79.3, 76.0, 71.1, 67.1, 64.7-64.6 (2×), 51.5, 39.3, 37.8, 30.2, 28.7, 26.9, 25.7, 23.1, 16.7-16.6 (2×); ³¹P NMR (162 MHz, CD₃OD) δ 7.7; HRMS calcd for C₃₅H₅₄N₄O₁₄P: 785.3374, found: m/z 785.3364 [M+H]⁺.

PZA(1Et)-7-CA-Amide 50

To a solution of 82 (52 mg, 0.066 mmol) in CH₃CN (15 mL) was added LiBr (23 mg, 0.26 mmol). After stirring under reflux for 12 h, the mixture was concentrated under reduced pressure. The residue was dissolved in CH₂Cl₂ (15 mL), and TFA (2 mL) was added. After stirring at room temperature for 3H, the mixture was concentrated under reduced pressure. The residue was purified by RP-18 reversed-phase column chromatography (MeOH/H₂O=5:95 to 40:60) and subjected to lyophilization to afford PZA(1Et)-7-CA-amide conjugate 50 (20 mg, 50%). C₂₅H₃₇N₄O₁₂P; Yellow solid; TLC (n-PrOH/H₂O=7:3) R_(f)=0.77; ¹H NMR (400 MHz, CD₃OD) δ 7.38 (1H, d, J=12.0 Hz), 7.01 (1H, s), 6.90 (1H, d, J=8.6 Hz), 6.76 (1H, d, J=8.1 Hz), 6.36 (1H, d, J=15.5 Hz), 5.44 (1H, d, J=8.6 Hz), 4.98 (1H, d, J=9.8 Hz), 4.41 (1H, d, J=10.4 Hz), 4.24 (1H, t, J=10.4 Hz), 4.08 (1H, q, J=7.1 Hz), 4.03-3.96 (2H, m), 3.92-3.87 (1H, m), 3.61-3.57 (1H, m), 3.49-3.44 (1H, m), 3.35 (2H, m), 3.18-3.08 (2H, m), 2.00 (3H, s), 1.79-1.68 (2H, m), 1.29 (3H, t, J=6.8 Hz); ¹³C NMR (100 MHz, CD₃OD) δ 174.3, 169.5, 158.1, 147.9 (d, ¹J_(c-p)=202 Hz), 142.3, 128.5, 122.2, 118.6, 116.6, 115.2, 105.3, 76.8, 71.1, 70.1, 64.7, 62.8, 53.2, 47.4, 39.4, 37.9, 30.5, 23.3, 17.4 (d, ³J_(c-p)=6.3 Hz); ³¹P NMR (162 MHz, CD₃OD) δ 2.3; HRMS calcd for C₂₅H₃₆N₄O₁₂P: 615.2067, found: m/z 615.2066 [M−H].

Example 34 Synthesis of PE-1-CA Conjugate 51 (FIG. 27) (−)-Methyl (1S,4R)-4-[(tert-butoxycarbonyl)amino]cyclopent-2-ene-1-carboxylate (85)

A mixture of (−)-(1R,4S)-2-azabicyclo[2.2.1]hept-5-en-3-one 83 (3.0 g, 27.5 mmol) and 1.25 M HCl in MeOH (59.0 mL, 73.8 mmol) was heated at reflux for 12 h. The mixture was concentrated under reduced pressure, and the residue was stirred with Et₂O (42 mL). The solids obtained were collected, washed with Et₂O, and dried under in vacuo to give 4.2 g (85%) of (1S,4R)-(−)-methyl-4-aminocyclopent-2-en-1-carboxylate hydrochloride (84). To a mixture of compound 84 (4.2 g, 23.6 mmol) and di-tert-butyl dicarbonate (10.86 mL, 47.3 mmol) in CH₂Cl₂ (120 mL) was added triethylamine (9.9 mL, 70.9 mmol) at 0° C. over a period of 2.5 h. The mixture was stirred for 1 h, and purified by flash column chromatography on silica gel (EtOAc/hexane, 1:6) to afford compound 85 (4.9 g, 86%). C₁₂H₁₉NO₄; white solid; ¹H NMR (400 MHz, CDCl₃) δ 5.87-5.82 (2H, m), 4.88 (1H, br s), 4.76 (1H, br s), 3.70 (3H, s), 3.48-3.45 (1H, m), 2.50 (1H, ddd, J=13.6, 8.4, 8.4 Hz), 1.86 (1H, ddd, J=13.6, 4.0, 4.0 Hz), 1.43 (9H, s); ESI-HRMS calcd. for C₁₂H₂₀NO₄: 242.1392, found: m/z 242.1395 [M+H]⁺.

(+)-Methyl (3aR,4R,6S,6aS)-4-[(tert-butoxycarbonyl)-amino]-3-(1-ethylpropyl)-4,5,6,6a-tetrahydro-3aH-cyclopenta[d]isoxazole-6-carboxylate (86)

To a mixture of olefin 85 (1.3 g, 5.4 mmol), 2-ethylbutanal oxime (4.2 g, 36.5 mmol), and Et₃N (0.34 mL, 2.4 mmol) in CH₂Cl₂ (12 mL) was added bleach (34.7 mL of 13% NaOCl aqueous solution, 72.7 mmol). The mixture was heated at reflux for 44 h, and monitored for completion by TLC. The mixture was extracted with CH₂Cl₂ (3×45 mL). The combined organic layers were washed with water and brine, dried over anhydrous Mg₂SO₄, filtered, and concentrated by rotary evaporation. The residue was purified by silica gel flash column chromatography (EtOAc/hexane gradients, 1:12 to 1:9 to 1:6) to give compound 86 (1.1 g, 57%). C₁₈H₃₀N₂O₅; pale yellow oil; ¹H NMR (400 MHz, CDCl₃) δ 5.57 (1H, d, J=7.2 Hz), 5.17 (1H, d, J=9.2 Hz), 4.19 (1H, br s), 3.73 (3H, s), 3.56 (1H, d, J=9.6 Hz), 3.16 (1H, d, J=7.6 Hz), 2.50-2.46 (1H, m), 2.12-2.05 (1H, m), 2.02-1.97 (1H, m), 1.74-1.55 (4H, m), 1.41 (9H, s), 0.92-0.84 (6H, m); ¹³C NMR (100 MHz, CDCl₃) δ 174.4, 160.5, 154.3, 87.0, 79.5, 63.6, 55.7, 52.7, 52.3, 40.7, 33.6, 28.7, 26.0, 24.3, 12.5, 11.2; ESI-HRMS calcd. for C₁₈H₃₁N₂O₅: 355.2233, found: m/z 355.2233 [M+H]⁺.

(−)-Methyl(1S,2S,3R,4R)-3-[(1S)-1-(Acetylamino)-2-ethylbutyl]-4-(tert-butoxycarbonyl)amino]-2-hydroxycyclopentanecarboxylate (88)

To a solution of 86 (951.1 mg, 2.7 mmol) in MeOH (20 mL) were added concentrated HCl (0.2 mL, 2.7 mmol) and PtO₂.H₂O (92.1 mg, 0.4 mmol). The mixture was stirred very vigorously at 100 psi hydrogen pressure for 8 h. The catalyst was removed by filtration, and the filtrate was concentrated to give (−)-methyl (1S,2S,3R,4R)-3-[(1S)-1-(amino)-2-ethylbutyl]-4-[(tert-butoxy-carbonyl)amino]-2-hydroxycyclopentanecarboxylate hydrochloride (87), which was used for acetylation. A solution of 87 (1.1 g, 2.7 mmol) in CH₂Cl₂ (10 mL) were added Et₃N (0.37 mL, 2.7 mmol) and Ac₂O (0.28 mL, 3.0 mmol) at room temperature. The mixture was stirred for 12 h, and then washed with water (10 mL). The water layer was back-extracted with CH₂Cl₂ (4 mL). The combined organic layers were washed with water and brine, dried over anhydrous Mg₂SO₄, and filtered. The filtrate was concentrated, and purified by silica gel flash column chromatography (EtOAc/hexane gradient, 1:3 to 1:1) to give compound 88 (730.8 mg, 68% from 86). C₂₀H₃₆N₂O₆; white solid; ¹H NMR (400 MHz, CDCl₃) δ 7.52 (1H, d, J=10 Hz), 4.76 (1H, d, J=9.2 Hz), 4.21 (1H, d, J=4.8 Hz), 4.13-4.09 (1H, m), 4.02-3.97 (1H, m), 3.68 (3H, s), 2.82-2.78 (1H, m), 2.50-2.42 (1H, m), 2.04 (3H, s), 1.98-1.95 (1H, m), 1.67 (1H, ddd, J=14.0, 8.4, 5.6 Hz), 1.42-1.19 (15H, m), 0.89-0.75 (6H, m); ¹³C NMR (100 MHz, CDCl₃) 174.7, 170.8, 155.4, 79.9, 77.6, 52.2, 52.0, 50.5, 49.1, 48.2, 43.6, 33.5, 28.7, 23.6, 22.2, 21.6, 11.0, 10.5; ESI-HRMS calcd. for C₂₀H₃₇N₂O₆: 401.2652, found: m/z 401.2648 [M+H]⁺.

(−)-Methyl(1S,2S,3R,4R)-3-[(1S)-1-(Acetylamino)-2-ethylbutyl]-4-({[(tert-butoxycarbonyl)amino][(tertbutoxycarbonyl)imino]methyl}amino)-2-hydroxycyclopentanecarboxylate (90)

A solution of 88 (818.9 mg, 2.04 mmol) in Et₂O (8.0 mL) was treated with 2 M HCl in Et₂O (3.3 mL, 6.54 mmol). The mixture was stirred at room temperature for 18 h. The solid obtained was collected, washed with Et₂O, and dried in vacuo to give 726.5 mg of 89, which used in the next step without further purification. A solution of 89 (726.5 mg, 2.16 mmol) in DMF (7.7 mL) was treated successively with Et₃N (1.1 mL, 7.55 mmol), 1,3-bis(tert-butoxycarbony)-2-methyl-2-thiopseudourea (626.3 mg, 2.16 mmol), and HgCl₂ (585.6 mg, 2.16 mmol). The mixture was stirred at room temperature for 19 h, diluted with EtOAc (200 mL), and filtered through a pad of Celite. The filtrate was washed with water and brine, dried over anhydrous Mg₂SO₄, and filtered. The filtrate was concentrated, and purified by silica gel flash column chromatography (EtOAc/hexane gradient, 1:2 to 2:3) to give compound 90 (730.8 mg, 83% from 88). C₂₆H₄₆N₄O₈; white foam; ¹H NMR (400 MHz, CDCl₃) δ 11.36 (1H, s), 8.69 (1H, d, J=10 Hz), 8.57 (1H, d, J=8.4 Hz), 4.48-4.39 (1H, m), 4.28-4.26 (1H, m), 4.22 (1H, s), 3.95 (1H, t, J=10 Hz), 3.70 (3H, s), 2.83-2.79 (1H, m), 2.52 (1H, ddd, J=13.6, 8.8, 8.8 Hz), 2.09 (3H, s), 2.08-2.07 (1H, m), 1.83 (1H, ddd, J=13.6, 8.4, 6.0 Hz), 1.48 (9H, s), 1.46 (9H, s), 1.42-1.21 (5H, m), 0.79 (3H, t, J=6.8 Hz), 0.74 (3H, t, J=7.2 Hz); ¹³C NMR (100 MHz, CDCl3) 174.8, 171.4, 162.4, 155.5, 152.8, 83.7, 79.8, 78.0, 52.2, 51.9, 50.8, 49.2, 48.5, 43.3, 33.8, 28.4, 28.1, 23.4, 22.5, 21.6, 10.7, 10.1; ESI-HRMS calcd. for C₂₆H₄₇N₄O₈: 543.3394, found: m/z 543.3399 [M+H]⁺.

(−)-(1S,2S,3R,4R)-3-[(1S)-1-(Acetylamino)-2-ethylbutyl]-4-({[(tert-butoxycarbonyl)amino][(tert-butoxycarbonyl)-imino]methyl}amino)-2-hydroxycyclopentanecarboxylic Acid (91)

A solution of 90 (921.7 mg, 1.70 mmol) in EtOH/THF (1:1, 6.2 mL) was treated with 1 M sodium hydroxide (6.1 mL, 6.11 mmol) at room temperature for 3 h. The mixture was concentrated. The residue was dissolved in water (12.3 mL), and acidified with acetic acid. The precipitate thus obtained was collected by filtration to give compound 91 (448.9 mg, 50%). C₂₅H₄₄N₄O₈; white solid; ¹H NMR (400 MHz, CDCl₃) δ 11.37 (1H, s), 8.80 (1H, d, J=10.4 Hz), 8.58 (1H, d, J=8.4 Hz), 4.47-4.36 (2H, m), 4.00 (1H, t, J=9.6 Hz), 2.82 (1H, t, J=6.8 Hz), 2.51 (1H, ddd, J=13.6, 8.8, 8.8 Hz), 2.12 (3H, s), 2.09-2.06 (1H, m), 1.94-1.85 (1H, m), 1.49 (9H, s), 1.47 (9H, s), 1.42-1.21 (6H, m), 0.80 (3H, t, J=6.8 Hz), 0.76 (3H, t, J=7.2 Hz); ¹³C NMR (100 MHz, CDCl₃) δ 177.5, 171.7, 162.4, 155.5, 152.8, 83.8, 79.8, 77.8, 51.6, 50.9, 49.7, 48.7, 43.4, 33.7, 28.4, 28.1, 23.3, 22.5, 21.6, 10.8, 10.3; ESI-HRMS calcd. for C₂₅H₄₅N₄O₈: 529.3237, found: m/z 529.3243 [M+H]⁺.

4-((1E)-3-(3-(((1S,2S,3R,4R)-3-((S)-1-acetamido-2-ethylbutyl)-4-(2,3-bis(tert-butoxycarbonyl)guanidino)-2-hydroxycyclopentanecarbonyl)oxy)propoxy)-3-oxoprop-1-en-1-yl)-1,2-phenylene diacetate (93)

A solution of 91 (88.9 mg, 0.17 mmol), 92 (59.6 mg, 0.18 mmol), EDCI (35.5 mg, 0.18 mmol) and 4-dimethylaminopyridine (22.6 mg, 0.18 mmol) in CH₂Cl₂ (5.0 mL) was stirred at room temperature for 2 h. The mixture was extracted with 1 M HCl, saturated NaHCO₃ and brine, dried over MgSO₄, concentrated under reduced pressure, and purified by silica gel flash column chromatography (EtOAc/hexane gradient, 1:2 to 2:1) to give compound 93 (45.2 mg, 32%). C₄₁H₆₀N₄O₁₄; transparent oil; ¹H NMR (400 MHz, CDCl₃) δ 11.37 (1H, s), 8.71 (1H, d, J=10.0 Hz), 8.58 (1H, d, J=8.4 Hz), 7.59 (1H, d, J=16.0 Hz), 7.38 (1H, dd, J=8.4, 2.4 Hz), 7.33 (1H, d, J=2.4 Hz), 7.20 (1H, d, J=8.4 Hz), 6.35 (1H, d, J=16.0 Hz), 4.48-4.43 (1H, m), 4.29-4.21 (5H, m), 3.97 (1H, t, J=9.6 Hz), 2.83 (1H, t, J=6.8 Hz), 2.55 (1H, ddd, J=13.6, 8.8, 8.8 Hz), 2.29 (6H, s), 2.13-2.08 (4H, m), 2.07-2.03 (2H, m), 1.89-1.82 (2H, m), 1.49 (9H, s), 1.47 (9H, s), 1.33-1.20 (4H, m), 0.80 (3H, t, J=7.2 Hz), 0.75 (3H, t, J=7.2 Hz); ¹³C NMR (100 MHz, CDCl₃) δ 174.3, 171.5, 167.8, 167.7, 166.1, 162.4, 155.5, 152.8, 143.3, 142.9, 142.2, 133.0, 126.3, 123.8, 122.6, 118.8, 83.8, 79.8, 78.0, 61.7, 61.1, 51.9, 50.8, 49.4, 48.6, 43.2, 33.8, 29.8, 28.4, 28.1, 23.4, 22.5, 21.6, 20.8, 10.7, 10.1; ESI-HRMS calcd. for C₄₁H₆₁N₄O₁₄: 833.4184, found: m/z 833.4185 [M+H]⁺.

PE-1-CA (51)

By a procedure similar to that for 43, saponification of 93 in aqueous K₂CO₃, followed by removal of t-Boc group with TFA, gave PE-1-CA (51).

Example 35 Synthesis of PE-2-CA Conjugate 52 (FIG. 28) (1S,2S,3R,4R)-Methyl-3-((S)-1-acetamido-2-ethylbutyl)-4-(2,3-bis(tert-butoxycarbony)guanidino)-2-(((4-nitrophenoxy)carbonyl)oxy)cyclopentanecarboxylate (94)

A solution of 90 (114.4 mg, 0.21 mmol), 4-nitrophenyl chloroformate (297.4 mg, 1.48 mmol) and 4-dimethylaminopyridine (297.4 mg, 1.48 mmol) in anhydrous pyridine (180.3 mg, 1.48 mmol) was stirred at room temperature for 19 h. After concentration, the mixture was added EtOAc, and then filtered. The filtrate was concentrated under reduced pressure, and purified by silica gel flash column chromatography (EtOAc/hexane gradient, 1:5 to 1:4) to give compound 94 (61.0 mg, 41%). C₃₃H₄₉N₅O₁₂; transparent oil; ¹H NMR (400 MHz, CDCl₃) δ 11.46 (1H, s), 8.56 (1H, d, J=8.4 Hz), 8.22 (2H, d, J=9.2 Hz), 8.13 (1H, d, J=10.4 Hz), 7.51 (2H, d, J=8.8 Hz), 5.49 (1H, d, J=5.6 Hz), 4.66 (1H, qui, J=8.8 Hz), 4.31-4.27 (1H, m), 3.72 (3H, s), 2.99-2.96 (1H, m), 2.59 (1H, ddd, J=13.6, 8.8, 8.8 Hz), 2.00 (3H, s), 1.93-1.80 (2H, m), 1.48 (9H, s), 1.45 (9H, s), 1.41-1.25 (5H, m), 0.83 (3H, t, J=7.2 Hz), 0.78 (3H, t, J=7.2 Hz); ESI-HRMS calcd. for C₃₃H₅₀N₅O₁₂: 708.3451, found: m/z 708.3450 [M+H]⁺.

(1S,2S,3R,4R)-Methyl-3-((S)-1-acetamido-2-ethylbutyl)-4-(2,3-bis(tert-butoxycarbonyl)guanidino)-2-(((3-(((E)-3-(3,4-bis(allyloxy)phenyl)acryloyl)oxy)propyl)carbamoyl)oxy)cyclopentanecarboxylate (96)

To a solution of caffeic acid derivative 95 (47.4 mg, 0.11 mmol) in CH₂Cl₂ (0.63 mL) was added TFA (0.63 mL, 8.22 mmol). After stirring at room temperature for 1 h, the mixture was concentrated under reduced pressure. The residue was dissolved in CH₃CN (0.91 mL), and then 1,3-bis(tert-butoxycarbony)-2-methyl-2-thiopseudourea (31.3 mg, 0.04 mmol) and Et₃N (0.03 mL, 0.22 mmol) were added. The mixture was stirred at room temperature for 2H, and then concentrated under reduced pressure. The residue was partitioned between CH₂Cl₂ and saturated NaHCO₃. The organic layer was dried over MgSO₄, filtered, and concentrated by rotary evaporation under reduced pressure. The crude material was purified by silica gel flash column chromatography (EtOAc/hexane gradient, 1:1 to 2:1) to give carbamate 96 (25.8 mg, 66%). C₄₅H₆₇N₅O₁₃; transparent oil; ¹H NMR (400 MHz, CDCl₃) δ 11.44 (1H, s), 8.57 (1H, d, J=8.0 Hz), 8.27 (1H, d, J=10.0 Hz), 7.56 (1H, d, J=15.6 Hz), 7.06-7.04 (2H, m), 6.84 (1H, d, J=8.8 Hz), 6.25 (1H, d, J=16.0 Hz), 6.11-6.00 (2H, m), 5.82 (1H, t, J=5.6 Hz), 5.43 (1H, dd, J=6.4, 1.6 Hz), 5.39 (1H, dd, J=6.4, 1.6 Hz), 5.27 (2H, dd, J=10.4, 1.6 Hz), 5.05-5.03 (1H, m), 4.62-4.61 (4H, m), 4.49 (1H, qui, J=8.4 Hz), 4.25-4.18 (2H, m), 4.17-4.09 (1H, m), 3.71 (3H, s), 3.26-3.23 (2H, m), 2.97-2.92 (1H, m), 2.44 (1H, ddd, J=12.8, 8.0, 8.0 Hz), 2.30-2.26 (1H, m), 2.07 (3H, s), 1.90-1.87 (2H, m), 1.82-1.75 (1H, m), 1.48 (9H, s), 1.45 (9H, s), 1.39-1.12 (5H, m), 0.84-0.77 (6H, m); ESI-HRMS calcd. for C₄₅H₆₈N₅O₁₃: 886.4814, found: m/z 886.4813 [M+H]⁺.

PE-2-CA (52)

By a procedure similar to that for 7, compound 96 was subject to saponification in aqueous KOH, followed by removal of the allyl and t-Boc groups respectively with Pd(PPh₃)₄ and TFA, to give PE-2-CA (52).

All publications and patent applications cited in this specification are herein incorporated by reference as if each individual publication or patent application were specifically and individually indicated to be incorporated by reference.

Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity of understanding, it will be readily apparent to those of ordinary skill in the art in light of the teachings of this invention that certain changes and modifications may be made thereto without departing from the spirit or scope of the appended claim.

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Other Embodiments

All of the features disclosed in this specification may be combined in any combination. Each feature disclosed in this specification may be replaced by an alternative feature serving the same, equivalent, or similar purpose. Thus, unless expressly stated otherwise, each feature disclosed is only an example of a generic series of equivalent or similar features.

From the above description, one skilled in the art can easily ascertain the essential characteristics of the present invention, and without departing from the spirit and scope thereof, can make various changes and modifications of the invention to adapt it to various usages and conditions. Thus, other embodiments are also within the claims. 

1. A chimeric drug compound comprising: a first moiety and a second moiety, wherein the first moiety is an anti-influenza agent and the second moiety is an anti-inflammatory agent, wherein the first and second moieties are conjugated via a linker as in Formula (A).


2. The compound of claim 1, wherein the anti-inflammatory agent is a non-steroidal anti-inflammatory agent.
 3. The compound of claim 2, wherein the non-steroidal anti-inflammatory agent is an acid anti-inflammatory agent.
 4. The compound of claim 1, wherein the anti-influenza agent is a neuraminidase inhibitor.
 5. The compound of claim 4, wherein the anti-influenza agent is zanamivir (ZA), phosphono-zanamivir (PZA), oseltamivir (OS), tamiphosphor (TP), or peramivir (PE), or a derivative thereof.
 6. The compound of claim 1, wherein one or more of the anti-influenza agents are conjugated with one or more of the anti-inflammatory agents via the linker.
 7. The compound of claim 6, wherein the linker comprises an optionally substituted C₁₋₂₀ hydrocarbon chain or optionally substituted cycloalkyl, wherein one or more carbon units are replaced with NR^(N), O or S, wherein R^(N) is hydrogen, optionally substituted alkyl, or a nitrogen protecting group.
 8. The compound of claim 6, wherein the linker is cleavable by an esterase.
 9. The compound of claim 6, wherein the linker is not cleavable by an esterase.
 10. The compound of claim 6, wherein the linker is cleavable in lysosome.
 11. The compound of claim 6, wherein the linker is not cleavable in lysosome.
 12. The compound of claim 5, wherein the compound is of formula (I) or (II):

or a pharmaceutically acceptable salt thereof; wherein: X₁ is —O—, —O(C═O)—, —NH—, —NHCO—, —O(C═O)NH—, —O(C═S)NH—, —NH(C═O)NH—, Or —NH(C═S)NH—; L is a linker selected from the group consisting of a bond, —(CH₂)_(n)—, —(CH₂)_(n)O—, —O(CH₂)_(n)—, —(CH₂)_(n)—C(O)—, —(CH₂)_(n)—NH—, —NH—(CH₂)_(n)—, —C(O)(CH₂)_(n)—, —(CH₂)_(n)—NHC(O)—, —C(O)(CH₂)_(n)—NHC(O)—, (CH₂)_(n)SCH₂C(O)—, and —(CH₂CH₂O)_(m)—; n is an integer from 1 to 8, inclusive; m is an integer from 1 to 4, inclusive; X₁ is covalently linked to L; Y is a non-steroidal anti-inflammatory agent selected from the group consisting of aceclofenac, acemetacin, acetyl-sailcylic acid, 5-aminoacetylsalicylic acid, aldlofenac, amfenac, bendazac, benoxaprofen, bermoprofen, bromfenac, 5-bromosalicylic acid acetate, bucloxic acid, butibufen, caffeic acid, carprofen, chromoglycate, cinmetacin, clindanac, clopirac, sodium diclofenac, diflunisal, 3,4-dihydroxybenzoic acid, enfenamic acid, etodolac, felbinac, fenbufen, fenclozic acid, fendosal, fenoprofen, fentiazac, flufenac, flufenamic acid, flunixin, flunoxaprofen, flurbiprofen, 1-hydroxynaphthoic acid, ibufenac, ibuprofen, indomethacin, indoprofen, isofezolac, isoxepac, ketoprofen, ketorolac, loxoprofen, meclofenamic acid, mefenamic acid, mesalazine, 3,4-methylenedioxycinnamic acid, metiazinic acid, mofezolac, montelukast, mycophenolic acid, naproxen, niflumic acid, olsalazine, oxaceprol, oxaprozin, pyrazolac, pirprofen, pranoprofen, protizinic acid, sulindac, suprofen, suxibutazone, tiaprofenic acid, tinoridine acid, tolfenamic acid, tolmetin, tropesin, xenbucin, ximoprofen, zaltoprofen, and zomepirac; Y is covalently linked to L via an acid group in the non-steroidal anti-inflammatory agent; B is an azido, a hydroxyl, an unsubstituted or substituted amino, or an unsubstituted or substituted guanidino, or the corresponding salts with an anionic counterion selected from the group consisting of chloride, bromide, iodide, acetate, trifluoroacetate, phosphate, diphosphate, nitrate, sulfate, benzenesulfonate, benzoate, salicylate, hydroxynaphthoate, fumarate, maleate, lactate, malate, succinate, tartrate, citrate, glutamate, and gluconate; each instance of R¹ and R⁵ is, independently, hydrogen or optionally substituted C₁₋₆ alkyl; each instance of R² and R³ is, independently, hydrogen, optionally substituted alkyl, or Optionally substituted acyl group; and R⁴ is —COCH₃, —COCF₃, —SO₂CH₃ or —SO₂CF₃.
 13. The compound of claim 5, wherein the compound is of formula (III) or (IV):

or a pharmaceutically acceptable salt thereof; wherein: X₂ is —O— or —NH—; X₂ is covalently linked to L; L is a linker selected from the group consisting of a bond, —(CH₂)_(n)—, —(CH₂)_(n)O—, —O(CH₂)_(n)—, —(CH₂)_(n)—C(O)—, —(CH₂)_(n)—NH—, —NH—(CH₂)_(n)—, —C(O)(CH₂)_(n)—, —(CH₂)_(n)—NHC(O)—, —C(O)(CH₂)_(n)—NHC(O)—, (CH₂)_(n)SCH₂C(O)—, and —(CH₂CH₂O)_(m)—; n is an integer from 1 to 8; m is an integer from 1 to 4; Y is a non-steroidal anti-inflammatory agent selected from the group consisting of aceclofenac, acemetacin, acetyl-sailcylic acid, 5-aminoacetylsalicylic acid, aldlofenac, amfenac, bendazac, benoxaprofen, bermoprofen, bromfenac, 5-bromosalicylic acid acetate, bucloxic acid, butibufen, caffeic acid, carprofen, chromoglycate, cinmetacin, clindanac, clopirac, sodium diclofenac, diflunisal, 3,4-dihydroxybenzoic acid, enfenamic acid, etodolac, felbinac, fenbufen, fenclozic acid, fendosal, fenoprofen, fentiazac, flufenac, flufenamic acid, flunixin, flunoxaprofen, flurbiprofen, 1-hydroxynaphthoic acid, ibufenac, ibuprofen, indomethacin, indoprofen, isofezolac, isoxepac, ketoprofen, ketorolac, loxoprofen, meclofenamic acid, mefenamic acid, mesalazine, 3,4-methylenedioxycinnamic acid, metiazinic acid, mofezolac, montelukast, mycophenolic acid, naproxen, niflumic acid, olsalazine, oxaceprol, oxaprozin, pyrazolac, pirprofen, pranoprofen, protizinic acid, sulindac, suprofen, suxibutazone, tiaprofenic acid, tinoridine acid, tolfenamic acid, tolmetin, tropesin, xenbucin, ximoprofen, zaltoprofen, and zomepirac; Y is covalently linked to L via an acid group in the non-steroidal anti-inflammatory agent; B is an azido, a hydroxyl, an unsubstituted or substituted amino, or an unsubstituted or substituted guanidino, or the corresponding salts with an anionic counterion selected from the group consisting of chloride, bromide, iodide, acetate, trifluoroacetate, phosphate, diphosphate, nitrate, sulfate, benzenesulfonate, benzoate, salicylate, hydroxynaphthoate, fumarate, maleate, lactate, malate, succinate, tartrate, citrate, glutamate, and gluconate; each instance of R² and R³ is, independently, hydrogen, optionally substituted alkyl, or Optionally substituted acyl group; R⁴ is —COCH₃, —COCF₃, —SO₂CH₃ or —SO₂CF₃; and R⁶ is hydrogen or optionally substituted C₁₋₆ alkyl.
 14. The compound of claim 5, wherein the compound is of formula (V) or (VI):

or a pharmaceutically acceptable salt thereof; wherein: X₁ is —O—, —O(C═O)—, —NH—, —NHCO—, —O(C═O)NH—, —O(C═S)NH—, —NH(C═O)NH—, Or —NH(C═S)NH—; L is a linker selected from the group consisting of a bond, —(CH₂)_(n)—, —(CH₂)_(n)O—, —O(CH₂)_(n)—, —(CH₂)_(n)—C(O)—, —(CH₂)_(n)—NH—, —NH—(CH₂)_(n)—, —C(O)(CH₂)_(n)—, —(CH₂)_(n)—NHC(O)—, —C(O)(CH₂)_(n)—NHC(O)—, (CH₂)_(n)SCH₂C(O)—, and —(CH₂CH₂O)_(m)—; n is an integer from 1 to 8; m is an integer from 1 to 4; X₁ is covalently linked to L; Y is a non-steroidal anti-inflammatory agent selected from the group consisting of aceclofenac, acemetacin, acetyl-sailcylic acid, 5-aminoacetylsalicylic acid, aldlofenac, amfenac, bendazac, benoxaprofen, bermoprofen, bromfenac, 5-bromosalicylic acid acetate, bucloxic acid, butibufen, caffeic acid, carprofen, chromoglycate, cinmetacin, clindanac, clopirac, sodium diclofenac, diflunisal, 3,4-dihydroxybenzoic acid, enfenamic acid, etodolac, felbinac, fenbufen, fenclozic acid, fendosal, fenoprofen, fentiazac, flufenac, flufenamic acid, flunixin, flunoxaprofen, flurbiprofen, 1-hydroxynaphthoic acid, ibufenac, ibuprofen, indomethacin, indoprofen, isofezolac, isoxepac, ketoprofen, ketorolac, loxoprofen, meclofenamic acid, mefenamic acid, mesalazine, 3,4-methylenedioxycinnamic acid, metiazinic acid, mofezolac, montelukast, mycophenolic acid, naproxen, niflumic acid, olsalazine, oxaceprol, oxaprozin, pyrazolac, pirprofen, pranoprofen, protizinic acid, sulindac, suprofen, suxibutazone, tiaprofenic acid, tinoridine acid, tolfenamic acid, tolmetin, tropesin, xenbucin, ximoprofen, zaltoprofen, and zomepirac; Y is covalently linked to L via an acid group in the non-steroidal anti-inflammatory agent; B is an azido, a hydroxyl, an unsubstituted or substituted amino, or an unsubstituted or substituted guanidino, or the corresponding salts with an anionic counterion selected from the group consisting of chloride, bromide, iodide, acetate, trifluoroacetate, phosphate, diphosphate, nitrate, sulfate, benzenesulfonate, benzoate, salicylate, hydroxynaphthoate, fumarate, maleate, lactate, malate, succinate, tartrate, citrate, glutamate, and gluconate; each instance of R¹, R⁵ and R⁶ is, independently, hydrogen or optionally substituted C₁₆ alkyl; each instance of R² and R³ is, independently, hydrogen, optionally substituted alkyl, or Optionally substituted acyl group; and R⁴ is —COCH₃, —COCF₃, —SO₂CH₃ or —SO₂CF₃.
 15. The compound of claim 5, wherein the compound is of formula (VII) or (VIII):

or a pharmaceutically acceptable salt thereof; wherein: Y is a non-steroidal anti-inflammatory agent selected from the group consisting of aceclofenac, acemetacin, acetyl-sailcylic acid, 5-aminoacetylsalicylic acid, aldlofenac, amfenac, bendazac, benoxaprofen, bermoprofen, bromfenac, 5-bromosalicylic acid acetate, bucloxic acid, butibufen, caffeic acid, carprofen, chromoglycate, cinmetacin, clindanac, clopirac, sodium diclofenac, diflunisal, 3,4-dihydroxybenzoic acid, enfenamic acid, etodolac, felbinac, fenbufen, fenclozic acid, fendosal, fenoprofen, fentiazac, flufenac, flufenamic acid, flunixin, flunoxaprofen, flurbiprofen, 1-hydroxynaphthoic acid, ibufenac, ibuprofen, indomethacin, indoprofen, isofezolac, isoxepac, ketoprofen, ketorolac, loxoprofen, meclofenamic acid, mefenamic acid, mesalazine, 3,4-methylenedioxycinnamic acid, metiazinic acid, mofezolac, montelukast, mycophenolic acid, naproxen, niflumic acid, olsalazine, oxaceprol, oxaprozin, pyrazolac, pirprofen, pranoprofen, protizinic acid, sulindac, suprofen, suxibutazone, tiaprofenic acid, tinoridine acid, tolfenamic acid, tolmetin, tropesin, xenbucin, ximoprofen, zaltoprofen, and zomepirac; Y is covalently linked to L via an acid group in the non-steroidal anti-inflammatory agent; each instance of R¹, R⁵ and R⁶ is, independently, hydrogen or optionally substituted C₁₆ alkyl; each instance of R² and R³ is, independently, hydrogen, optionally substituted alkyl, or Optionally substituted acyl group; and R⁴ is —COCH₃, —COCF₃, —SO₂CH₃ or —SO₂CF₃.
 16. The compound of claim 5, wherein the compound is of formula (IX) or (X):

or a pharmaceutically acceptable salt thereof; wherein: X₂ is —O— or —NH—; X₂ is covalently linked to L; L is a linker selected from the group consisting of a bond, —(CH₂)_(n)—, —(CH₂)_(n)O—, —O(CH₂)_(n)—, —(CH₂)_(n)—C(O)—, —(CH₂)_(n)—NH—, —NH—(CH₂)_(n)—, —C(O)(CH₂)_(n)—, —(CH₂)_(n)—NHC(O)—, —C(O)(CH₂)_(n)—NHC(O)—, (CH₂)_(n)SCH₂C(O)—, and —(CH₂CH₂O)_(m)—; n is an integer from 1 to 8; m is an integer from 1 to 4; Y is a non-steroidal anti-inflammatory agent selected from the group consisting of aceclofenac, acemetacin, acetyl-sailcylic acid, 5-aminoacetylsalicylic acid, aldlofenac, amfenac, bendazac, benoxaprofen, bermoprofen, bromfenac, 5-bromosalicylic acid acetate, bucloxic acid, butibufen, caffeic acid, carprofen, chromoglycate, cinmetacin, clindanac, clopirac, sodium diclofenac, diflunisal, 3,4-dihydroxybenzoic acid, enfenamic acid, etodolac, felbinac, fenbufen, fenclozic acid, fendosal, fenoprofen, fentiazac, flufenac, flufenamic acid, flunixin, flunoxaprofen, flurbiprofen, 1-hydroxynaphthoic acid, ibufenac, ibuprofen, indomethacin, indoprofen, isofezolac, isoxepac, ketoprofen, ketorolac, loxoprofen, meclofenamic acid, mefenamic acid, mesalazine, 3,4-methylenedioxycinnamic acid, metiazinic acid, mofezolac, montelukast, mycophenolic acid, naproxen, niflumic acid, olsalazine, oxaceprol, oxaprozin, pyrazolac, pirprofen, pranoprofen, protizinic acid, sulindac, suprofen, suxibutazone, tiaprofenic acid, tinoridine acid, tolfenamic acid, tolmetin, tropesin, xenbucin, ximoprofen, zaltoprofen, and zomepirac; Y is covalently linked to L via an acid group in the non-steroidal anti-inflammatory agent; B is an azido, a hydroxyl, an unsubstituted or substituted amino, or an unsubstituted or substituted guanidino, or the corresponding salts with an anionic counterion selected from the group consisting of chloride, bromide, iodide, acetate, trifluoroacetate, phosphate, diphosphate, nitrate, sulfate, benzenesulfonate, benzoate, salicylate, hydroxynaphthoate, fumarate, maleate, lactate, malate, succinate, tartrate, citrate, glutamate, and gluconate; R⁴ is —COCH₃, —COCF₃, —SO₂CH₃ or —SO₂CF₃.
 17. The compound of claim 5, wherein the compound is of formula (XI) or (XII):

or a pharmaceutically acceptable salt thereof; wherein: X₁ is —O—, —O(C═O)—, —NH—, —NHCO—, —O(C═O)NH—, —O(C═S)NH—, —NH(C═O)NH—, Or —NH(C═S)NH—; X₂ is —O— or —NH—; X₁ or X₂ is covalently linked to L; L is a linker selected from the group consisting of a bond, —(CH₂)_(n)—, —(CH₂)_(n)O—, —O(CH₂)_(n)—, —(CH₂)_(n)—C(O)—, —(CH₂)_(n)—NH—, —NH—(CH₂)_(n)—, —C(O)(CH₂)_(n)—, —(CH₂)_(n)—NHC(O)—, —C(O)(CH₂)_(n)—NHC(O)—, (CH₂)_(n)SCH₂C(O)—, and —(CH₂CH₂O)_(m)—; n is an integer from 1 to 8; m is an integer from 1 to 4; Y is a non-steroidal anti-inflammatory agent selected from the group consisting of aceclofenac, acemetacin, acetyl-sailcylic acid, 5-aminoacetylsalicylic acid, aldlofenac, amfenac, bendazac, benoxaprofen, bermoprofen, bromfenac, 5-bromosalicylic acid acetate, bucloxic acid, butibufen, caffeic acid, carprofen, chromoglycate, cinmetacin, clindanac, clopirac, sodium diclofenac, diflunisal, 3,4-dihydroxybenzoic acid, enfenamic acid, etodolac, felbinac, fenbufen, fenclozic acid, fendosal, fenoprofen, fentiazac, flufenac, flufenamic acid, flunixin, flunoxaprofen, flurbiprofen, 1-hydroxynaphthoic acid, ibufenac, ibuprofen, indomethacin, indoprofen, isofezolac, isoxepac, ketoprofen, ketorolac, loxoprofen, meclofenamic acid, mefenamic acid, mesalazine, 3,4-methylenedioxycinnamic acid, metiazinic acid, mofezolac, montelukast, mycophenolic acid, naproxen, niflumic acid, olsalazine, oxaceprol, oxaprozin, pyrazolac, pirprofen, pranoprofen, protizinic acid, sulindac, suprofen, suxibutazone, tiaprofenic acid, tinoridine acid, tolfenamic acid, tolmetin, tropesin, xenbucin, ximoprofen, zaltoprofen, and zomepirac; Y is covalently linked to L via an acid group in the non-steroidal anti-inflammatory agent; B is an azido, a hydroxyl, an unsubstituted or substituted amino, or an unsubstituted or substituted guanidino, or the corresponding salts with an anionic counterion selected from the group consisting of chloride, bromide, iodide, acetate, trifluoroacetate, phosphate, diphosphate, nitrate, sulfate, benzenesulfonate, benzoate, salicylate, hydroxynaphthoate, fumarate, maleate, lactate, malate, succinate, tartrate, citrate, glutamate, and gluconate; each instance of R¹ and R⁷ is, independently, hydrogen or optionally substituted C₁₋₆ alkyl; and R⁴ is —COCH₃, —COCF₃, —SO₂CH₃ or —SO₂CF₃.
 18. The compound of claim 12, wherein the compound is selected from the group consisting of:


19. The compound of claim 13, wherein the compound is selected from the group consisting of:


20. The compound of claim 14, wherein the compound is selected from the group consisting of:


21. The compound of claim 14, wherein the compound is selected from the group consisting of:


22. The compound of claim 14, wherein the compound is selected from the group consisting of:


23. The compound of claim 15, wherein the compound is selected from the group consisting of:


24. A dual-active pharmaceutical composition for simultaneously inhibiting influenza virus and inflammation, the composition comprising: a compound comprising: a first moiety and a second moiety, wherein the first moiety is an anti-influenza agent and the second moiety is an anti-inflammatory agent, and wherein the first and second moieties are conjugated via a linker as in Formula (A).

or a pharmaceutically acceptable salt thereof; and a pharmaceutically acceptable excipient.
 25. The pharmaceutical composition of claim 24, wherein the anti-influenza agent is an influenza virus neuraminidase inhibitor.
 26. The composition of claim 24, wherein the anti-inflammatory agent is a pro-inflammatory cytokine inhibitor.
 27. The composition of claim 24, wherein the compound inhibits both human and avian influenza virus.
 28. The composition of claim 24, wherein the compound inhibits at least one of H1N1 and H5N1 strains of influenza virus.
 29. The composition of claim 25, wherein the neuraminidase is a wild-type neuraminidase.
 30. The composition of claim 25, wherein the neuraminidase is oseltamivir-resistant.
 31. A method for treating influenza infection in a subject, the method comprising: administering to a subject in need thereof a therapeutically effective amount of the pharmaceutical composition of claim
 24. 32. The method of claim 31, wherein the subject has, is suspected of having, or is at risk for influenza virus infection. 